Reviewed, UniProtKB/Swiss-Prot Q0IQU1 (LAC22_ORYSJ)

Names and origin

Protein names

Recommended name:
    Laccase-22
    EC=1.10.3.2
Alternative name(s):
    Benzenediol:oxygen oxidoreductase 22
    Urishiol oxidase 22
    Diphenol oxidase 22

Gene names

Name:	LAC22
Ordered Locus Names:	Os11g0708100, LOC_Os11g48060
ORF Names:	OsJ_033548

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Lignin degradation and detoxification of lignin-derived products By similarity.
Catalytic activity	4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O.
Cofactor	Binds 4 copper ions per monomer By similarity.
Subcellular location	Secreted › extracellular space › apoplastPotential.
Sequence similarities	Belongs to the multicopper oxidase family. Contains 3 plastocyanin-like domains.
Sequence caution	The sequence BAF28924.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Lignin degradation
Cellular component	Apoplast Secreted
Domain	Repeat Signal
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	copper ion binding Inferred from electronic annotation. Source: InterPro laccase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 564	539	Laccase-22		PRO_0000291909
Regions
	Domain	36 – 152	117	Plastocyanin-like 1
	Domain	162 – 314	153	Plastocyanin-like 2
	Domain	414 – 548	135	Plastocyanin-like 3
Sites
	Metal binding	86	1	Copper 1 By similarity
	Metal binding	88	1	Copper 2 By similarity
	Metal binding	131	1	Copper 2 By similarity
	Metal binding	133	1	Copper 3 By similarity
	Metal binding	465	1	Copper 4 By similarity
	Metal binding	468	1	Copper 1 By similarity
	Metal binding	470	1	Copper 3 By similarity
	Metal binding	527	1	Copper 3 By similarity
	Metal binding	528	1	Copper 4 By similarity
	Metal binding	529	1	Copper 2 By similarity
	Metal binding	533	1	Copper 4 By similarity
Amino acid modifications
	Glycosylation	41	1	N-linked (GlcNAc...) Potential
	Glycosylation	82	1	N-linked (GlcNAc...) Potential
	Glycosylation	118	1	N-linked (GlcNAc...) Potential
	Glycosylation	191	1	N-linked (GlcNAc...) Potential
	Glycosylation	302	1	N-linked (GlcNAc...) Potential
	Glycosylation	331	1	N-linked (GlcNAc...) Potential
	Glycosylation	379	1	N-linked (GlcNAc...) Potential
	Glycosylation	389	1	N-linked (GlcNAc...) Potential
	Glycosylation	424	1	N-linked (GlcNAc...) Potential
	Glycosylation	437	1	N-linked (GlcNAc...) Potential
	Glycosylation	447	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0IQU1-1 [UniParc]. Last modified June 26, 2007. Version 2. Checksum: C88700E8EDA0C0D2	FASTA	564	62,042
	10 20 30 40 50 60 MAVLPESRRL SLLLMAACFL LQALSAHAIT RHYKFNVVMR NMTRLCSTKP ILTVNGKFPG 70 80 90 100 110 120 PTLYAREGDN VLVKVVNHVA HNVTIHWHGV RQIRTGWYDG PAYITQCPIQ PGSSFLYNFT 130 140 150 160 170 180 ITGQRGTLLW HAHINWLRAT VHGAIVILPK LGVPYPFPAP HKEAVIVLGE WWKEDTETVI 190 200 210 220 230 240 NQAMQLGVGP NISDSHTING HPGPLSECAS SQDGFKLSVE NGKTYMLRII NAALNDDLFF 250 260 270 280 290 300 KVAGHELTVV EVDAVYTKPF KTDTLLITPG QTTNVLVRAN QGAGRYLLSV SPFMDAPVQV 310 320 330 340 350 360 DNKTGTATLH YANTVSSSMA SLTLVKPPPQ NATHIVSKFT DSLHSLNSKE YPANVPQTVD 370 380 390 400 410 420 HSLLLTVGVG VNPCPSCING TRVVGTINNV TFIMPSTPIL QAHYYNIPGV FTEDFPATPL 430 440 450 460 470 480 HKFNYTGSGP KNLQTMNGTR VYRLPYNASV QVVLQDTGII SPESHPIHLH GFNFFVVGKG 490 500 510 520 530 540 VGNYNPRTSP STFNLIDPIE RNTIGVPTGG WTAIRFRSDN PGVWFMHCHF EVHTSWGLKM 550 560 AFVVDNGKRP SETLIPPPKD LPQC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications." The rice chromosomes 11 and 12 sequencing consortia BMC Biol. 3:20-20(2005) [PubMed: 16188032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC133008 Genomic DNA. Translation: AAX95423.1. DP000010 Genomic DNA. Translation: ABA95532.1. AP008217 Genomic DNA. Translation: BAF28924.1. Sequence problems. CM000148 Genomic DNA. Translation: EAZ19339.1.
RefSeq	NP_001068561.1.
UniGene	Os.87771
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4351231.
KEGG	osa:4351231.
Organism-specific databases
Gramene	Q53NW2.
Family and domain databases
InterPro	IPR001117. Cu-oxidase. IPR011706. Cu-oxidase_2. IPR011707. Cu-oxidase_3. IPR002355. Cu_oxidase_Cu_BS. IPR008972. Cupredoxin. IPR017761. Laccase. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 1 hit.
Pfam	PF00394. Cu-oxidase. 1 hit. PF07731. Cu-oxidase_2. 1 hit. PF07732. Cu-oxidase_3. 1 hit. [Graphical view]
PROSITE	PS00079. MULTICOPPER_OXIDASE1. 1 hit. PS00080. MULTICOPPER_OXIDASE2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC22_ORYSJ

Accession

Primary (citable) accession number: Q0IQU1
Secondary accession number(s): Q53NW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	June 26, 2007
Last modified:	November 25, 2008
This is version 20 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)