Q0IQK9 (NLTP1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-specific lipid-transfer protein 1
Short name=LTP 1
Short name=PAPI

Gene names

Name:	LTP
Ordered Locus Names:	Os12g0115100, LOC_Os12g02320
ORF Names:	OsJ_033644

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	116 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues.
Tissue specificity	Aleurone (external part) of the seeds.
Sequence similarities	Belongs to the plant LTP family.
Caution	Was originally thought to be an inhibitor of alpha-amylase or of a protease and was known as PAPI: probable alpha-amylase/protease inhibitor.

Ontologies

Keywords
Biological process	Transport
Domain	Signal
Ligand	Lipid-binding
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	lipid transport Inferred from electronic annotation. Source: InterPro
Molecular function	lipid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 25

25

Ref.5

Chain

26 – 116

91

Non-specific lipid-transfer protein 1

PRO_0000018391

Amino acid modifications

Disulfide bond

28 ↔ 75

Disulfide bond

38 ↔ 52

Disulfide bond

53 ↔ 98

Disulfide bond

73 ↔ 112

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

116

Helix Strand Turn

Details...

Helix

28 – 35

8

Helix

36 – 38

3

Helix

39 – 42

4

Helix

50 – 62

13

Helix

66 – 81

16

Helix

88 – 92

5

Helix

94 – 98

5

Helix

112 – 114

3

Sequences

	Sequence		Length	Mass (Da)
	Q0IQK9 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 54612FE0D79F9D5D	FASTA	116	11,345
	10 20 30 40 50 60 MARAQLVLVA LVAALLLAAP HAAVAITCGQ VNSAVGPCLT YARGGAGPSA ACCSGVRSLK 70 80 90 100 110 AAASTTADRR TACNCLKNAA RGIKGLNAGN AASIPSKCGV SVPYTISASI DCSRVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications." The rice chromosomes 11 and 12 sequencing consortia BMC Biol. 3:20-20(2005) [PubMed: 16188032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"Amino acid sequence of a probable amylase/protease inhibitor from rice seeds." Yu Y.G., Chung C.H., Fowler A., Suh S.W. Arch. Biochem. Biophys. 265:466-475(1988) [PubMed: 2458699] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-116. Tissue: Seed.
[6]	"Rice non-specific lipid transfer protein: the 1.6-A crystal structure in the unliganded state reveals a small hydrophobic cavity." Lee J.Y., Min K., Cha H., Shin D.H., Hwang K.Y., Suh S.W. J. Mol. Biol. 276:437-448(1998) [PubMed: 9512714] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS). Tissue: Seed.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

DP000011 Genomic DNA. Translation: ABA96284.1.
AP008218 Genomic DNA. Translation: BAF29006.1.
CM000149 Genomic DNA. Translation: EAZ19435.1.

RefSeq

NP_001065987.1. NM_001072519.1.

UniGene

Os.8629.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BV2	NMR	-	A	26-116	[»]
1RZL	X-ray	1.60	A	26-116	[»]
1UVA	X-ray	2.50	A	26-116	[»]
1UVB	X-ray	2.10	A	26-116	[»]
1UVC	X-ray	2.00	A/B	26-116	[»]

ProteinModelPortal

Q0IQK9.

SMR

Q0IQK9. Positions 26-116.

ModBase

Search...

Protein-protein interaction databases

STRING

Q0IQK9.

Protein family/group databases

Allergome

2788. Ory s 14.

Proteomic databases

PRIDE

Q0IQK9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

LOC_Os12g02320.1; LOC_Os12g02320.1; LOC_Os12g02320.

GeneID

4351318.

KEGG

osa:4351318.

Organism-specific databases

Gramene

P23096.

Phylogenomic databases

GeneTree

EPGT00050000004582.

HOGENOM

HBG744832.

OMA

TESTIQC.

PhylomeDB

Q0IQK9.

ProtClustDB

CLSN2698338.

Family and domain databases

InterPro

IPR016140. Bifunc_inhib/LTP/seed_store.
IPR013770. Lipid_transfer_prot_hlx-dom.
IPR003612. LTP/seed_store/tryp_amyl_inhib.
IPR000528. Plant_LTP.
[Graphical view]

Gene3D

G3DSA:1.10.110.10. LPT_helical. 1 hit.

Pfam

PF00234. Tryp_alpha_amyl. 1 hit.
[Graphical view]

PRINTS

PR00382. LIPIDTRNSFER.

SMART

SM00499. AAI. 1 hit.
[Graphical view]

SUPFAM

SSF47699. Bifunc_inhib/LTP/seed_store. 1 hit.

PROSITE

PS00597. PLANT_LTP. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

NLTP1_ORYSJ

Accession

Primary (citable) accession number: Q0IQK9
Secondary accession number(s): O22484 , P23096, P93434, Q2QYL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	October 3, 2006
Last modified:	December 14, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program