Reviewed, UniProtKB/Swiss-Prot Q0IQJ7 (MAN8_ORYSJ)

Names and origin

Protein names

Recommended name:
    Mannan endo-1,4-beta-mannosidase 8
    EC=3.2.1.78
Alternative name(s):
    Beta-mannanase 8
    Endo-beta-1,4-mannanase 8
    OsMAN8

Gene names

Name:	MAN8
Ordered Locus Names:	Os12g0117300, LOC_Os12g02520

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Tissue specificity	Expressed in stems and leaves and seeds. Ref.4
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.
Sequence caution	The sequence BAF29018.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Mannan endo-1,4-beta-mannosidase 8		PRO_0000277489
Sites
	Active site	173	1	Proton donor By similarity
	Active site	293	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0IQJ7-1 [UniParc]. Last modified February 6, 2007. Version 2. Checksum: 4FB08926901C47EE	FASTA	372	41,839
	10 20 30 40 50 60 MVECSGTQLW ASGRPFIIHG FNTYWLMSFA ADQATRPRVT AAIAEAAEAG LNVCRTWAFS 70 80 90 100 110 120 DGGYRALQTV PFHYDEDVFQ ALDFVVSEAK RHNMRLILSL CNNWEDYGGK AQYVRWGKEA 130 140 150 160 170 180 GLDLTSEDDF FSDPTIKSYY KAFVEAVVTR INTVTNETYK DDPTILAWEL INEPRCPSDP 190 200 210 220 230 240 SGDTLQAWIE EMASYVKSID PVHLLEIGIE GFYGLSTPEL LPVNPDEYSG HAGTDFIRNH 250 260 270 280 290 300 QAPGIDLASI HVYSDTWLPH SIKENHLQFV DKWMQQHIHD AANLLGMPIV VGEFGVSVKD 310 320 330 340 350 360 GKFGNEFRED FMKTVYRIFL SSWKEGVIGG GCLLWQLFPE GAEHMDDGYA VIFAKSPSTL 370 SLLANHLRCL EC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications." The rice chromosomes 11 and 12 sequencing consortia BMC Biol. 3:20-20(2005) [PubMed: 16188032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar." Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F. Funct. Integr. Genomics 7:1-16(2007) [PubMed: 16897088] [Abstract] Cited for: GENE FAMILY, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DP000011 Genomic DNA. Translation: ABA95648.1. AP008218 Genomic DNA. Translation: BAF29018.1. Sequence problems.
RefSeq	NP_001065999.1.
UniGene	Os.89197
3D structure databases
SMR	Q0IQJ7. Positions 2-370.
ModBase	Search...
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Genome annotation databases
GeneID	4351332.
KEGG	osa:4351332.
Organism-specific databases
Gramene	Q2QYI9.
Family and domain databases
InterPro	IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAN8_ORYSJ

Accession

Primary (citable) accession number: Q0IQJ7
Secondary accession number(s): Q2QYI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	February 6, 2007
Last modified:	January 19, 2010
This is version 27 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)