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Q0IQJ7 (MAN8_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mannan endo-1,4-beta-mannosidase 8

EC=3.2.1.78
Alternative name(s):
Beta-mannanase 8
Endo-beta-1,4-mannanase 8
OsMAN8
Gene names
Name:MAN8
Ordered Locus Names:Os12g0117300, LOC_Os12g02520
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Tissue specificity

Expressed in stems and leaves and seeds. Ref.4

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Sequence caution

The sequence BAH95489.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionmannan endo-1,4-beta-mannosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 372372Mannan endo-1,4-beta-mannosidase 8
PRO_0000277489

Sites

Active site1731Proton donor By similarity
Active site2931Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0IQJ7 [UniParc].

Last modified February 6, 2007. Version 2.
Checksum: 4FB08926901C47EE

FASTA37241,839
        10         20         30         40         50         60 
MVECSGTQLW ASGRPFIIHG FNTYWLMSFA ADQATRPRVT AAIAEAAEAG LNVCRTWAFS 

        70         80         90        100        110        120 
DGGYRALQTV PFHYDEDVFQ ALDFVVSEAK RHNMRLILSL CNNWEDYGGK AQYVRWGKEA 

       130        140        150        160        170        180 
GLDLTSEDDF FSDPTIKSYY KAFVEAVVTR INTVTNETYK DDPTILAWEL INEPRCPSDP 

       190        200        210        220        230        240 
SGDTLQAWIE EMASYVKSID PVHLLEIGIE GFYGLSTPEL LPVNPDEYSG HAGTDFIRNH 

       250        260        270        280        290        300 
QAPGIDLASI HVYSDTWLPH SIKENHLQFV DKWMQQHIHD AANLLGMPIV VGEFGVSVKD 

       310        320        330        340        350        360 
GKFGNEFRED FMKTVYRIFL SSWKEGVIGG GCLLWQLFPE GAEHMDDGYA VIFAKSPSTL 

       370 
SLLANHLRCL EC 

« Hide

References

« Hide 'large scale' references
[1]"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications."
The rice chromosomes 11 and 12 sequencing consortia
BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[4]"The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar."
Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F.
Funct. Integr. Genomics 7:1-16(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DP000011 Genomic DNA. Translation: ABA95648.1.
AP008218 Genomic DNA. Translation: BAH95489.1. Sequence problems.
RefSeqNP_001176761.1. NM_001189832.1.
UniGeneOs.100825.

3D structure databases

ProteinModelPortalQ0IQJ7.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID9271116.
KEGGosa:9271116.

Organism-specific databases

GrameneQ0IQJ7.

Phylogenomic databases

eggNOGCOG3934.
OMAEHMDDGY.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMAN8_ORYSJ
AccessionPrimary (citable) accession number: Q0IQJ7
Secondary accession number(s): C7J9Y7, Q2QYI9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: February 6, 2007
Last modified: May 14, 2014
This is version 56 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries