Q0INY7 (RBS1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribulose bisphosphate carboxylase small chain, chloroplastic
Short name=Ribulose bisphosphate carboxylase small chain C
Short name=RuBisCO small subunit
Short name=RuBisCO small subunit C
EC=4.1.1.39

Gene names

Name:	RBCS
Synonyms:	RBCS-C
Ordered Locus Names:	Os12g0274700, LOC_Os12g17600
ORF Names:	OsJ_016909

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	175 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site By similarity.
Catalytic activity	2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. 3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.
Subunit structure	8 large chains + 8 small chains.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the RuBisCO small chain family.
Sequence caution	The sequence CAA30393.1 differs from that shown. Reason: Frameshift at several positions. The sequence CAA30393.1 differs from that shown. Reason: Sequencing errors.

Ontologies

Keywords
Biological process	Calvin cycle Carbon dioxide fixation Photorespiration Photosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Lyase Monooxygenase Oxidoreductase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	photorespiration Inferred from electronic annotation. Source: UniProtKB-KW reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW response to blue light Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to cold Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to far red light Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to red light Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	apoplast Inferred from electronic annotation. Source: EnsemblPlants/Gramene cell wall Inferred from electronic annotation. Source: EnsemblPlants/Gramene chloroplast envelope Inferred from electronic annotation. Source: EnsemblPlants/Gramene chloroplast stroma Inferred from electronic annotation. Source: EnsemblPlants/Gramene chloroplast thylakoid membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene cytosolic ribosome Inferred from electronic annotation. Source: EnsemblPlants/Gramene thylakoid lumen Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	copper ion binding Inferred from electronic annotation. Source: EnsemblPlants/Gramene monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW ribulose-bisphosphate carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 47

47

Chloroplast Ref.8

Chain

48 – 175

128

Ribulose bisphosphate carboxylase small chain, chloroplastic

PRO_0000031537

Regions

Region

60 – 64

5

Interaction with large subunit

Secondary structure

1

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175

Helix Strand Turn

Details...

Beta strand

53 – 55

3

Turn

61 – 64

4

Helix

70 – 82

13

Beta strand

86 – 93

8

Beta strand

109 – 111

3

Beta strand

114 – 117

4

Helix

126 – 139

14

Beta strand

143 – 151

9

Turn

152 – 155

4

Beta strand

156 – 164

9

Sequences

	Sequence		Length	Mass (Da)
	Q0INY7 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: E95B68AC3178A7D7	FASTA	175	19,647
	10 20 30 40 50 60 MAPSVMASSA TTVAPFQGLK STAGMPVARR SGNSSFGNVS NGGRIRCMQV WPIEGIKKFE 70 80 90 100 110 120 TLSYLPPLTV EDLLKQIEYL LRSKWVPCLE FSKVGFVYRE NHRSPGYYDG RYWTMWKLPM 130 140 150 160 170 FGCTDATQVL KELEEAKKAY PDAFVRIIGF DNVRQVQLIS FIAYKPPGCE ESGGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit gene (rbcS) in rice." Xie Y., Wu R. Nucleic Acids Res. 16:7749-7749(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Classification and nucleotide sequence of cDNA encoding the small subunit of ribulose-1,5-bisphosphate carboxylase from rice." Matsuoka M., Kano-Murakami Y., Tanaka Y., Ozeki Y., Yamamoto N. Plant Cell Physiol. 29:1015-1022(1988) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare.
[3]	"Molecular cloning and characterization of cDNA encoding the small subunit of ribulose 1,5-bisphosphate carboxylase in rice." Yoon U.H., Hahn J.H., Yun C.-H., Eun M.Y. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Ilpoombyeo.
[4]	"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications." The rice chromosomes 11 and 12 sequencing consortia BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[6]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[7]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[8]	"Proteomic analysis of rice leaf, stem and root tissues during growth course." Nozu Y., Tsugita A., Kamijo K. Proteomics 6:3665-3670(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 48-54, MASS SPECTROMETRY. Strain: cv. Nipponbare.
[9]	"Crystal structure of activated rice RuBisCO complexed with 2-carboxyarabinitol-1,5-bisphosphate." Mizohata E., Matsumura H., Ueno T., Ishida H., Inoue T., Mae T., Kai Y. Submitted (NOV-2004) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS). Strain: cv. Notohikari. Tissue: Leaf.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X07515 Genomic DNA. Translation: CAA30393.1. Sequence problems.
D00643 mRNA. Translation: BAA00538.1.
AF052305 mRNA. Translation: AAC14064.1.
DP000011 Genomic DNA. Translation: ABA97056.1.
AP008218 Genomic DNA. Translation: BAF29578.1.
CM000142 Genomic DNA. Translation: EAZ33426.1.

PIR

RKRZS9. JA0070.
RKRZS. S01235.

RefSeq

NP_001066559.1. NM_001073091.1.

UniGene

Os.22440.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WDD	X-ray	1.35	S/W	48-175	[»]
3AXK	X-ray	1.90	S/T	48-175	[»]
3AXM	X-ray	1.65	S/T/U/V/W/X/Y/Z	48-175	[»]

ProteinModelPortal

Q0INY7.

SMR

Q0INY7. Positions 49-169.

ModBase

Search...

Proteomic databases

PaxDb

Q0INY7.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

LOC_Os12g17600.1; LOC_Os12g17600.1; LOC_Os12g17600.

GeneID

4351966.

KEGG

dosa:Os12t0274700-01.
osa:4351966.

Organism-specific databases

Gramene

Q0INY7.

Phylogenomic databases

eggNOG

COG4451.

KO

K01602.

OMA

AFPATRK.

ProtClustDB

PLN02289.

Gene expression databases

ArrayExpress

Q0INY7.

Family and domain databases

Gene3D

3.30.190.10. 1 hit.

InterPro

IPR024681. RuBisCO_sc.
IPR000894. RuBisCO_sc_dom.
IPR024680. RuBisCO_ssu_N.
[Graphical view]

Pfam

PF12338. RbcS. 1 hit.
PF00101. RuBisCO_small. 1 hit.
[Graphical view]

PRINTS

PR00152. RUBISCOSMALL.

SUPFAM

SSF55239. RuBisCO_small. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q0INY7.

Entry information

Entry name

RBS1_ORYSJ

Accession

Primary (citable) accession number: Q0INY7
Secondary accession number(s): O65105 , P05347, P18567, Q2QU37

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 3, 2006
Last modified:	April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program