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Protein

E3 ubiquitin-protein ligase SPL11

Gene

SPL11

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that negatively regulates programmed cell death and disease resistance. Participates in flowering time control by mediating ubiquitination and subsequent proteasomal degradation of SPIN1.2 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  • defense response Source: UniProtKB-KW
  • flower development Source: UniProtKB-KW
  • negative regulation of defense response Source: UniProtKB
  • negative regulation of programmed cell death Source: UniProtKB
  • protein ubiquitination Source: UniProtKB
  • regulation of long-day photoperiodism, flowering Source: UniProtKB

Keywordsi

Molecular functionTransferase
Biological processFlowering, Plant defense, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase SPL11 (EC:2.3.2.27)
Alternative name(s):
Cell death-related protein SPL11
Protein spotted leaf 11
RING-type E3 ubiquitin transferase SPL11Curated
Gene namesi
Name:SPL11
Ordered Locus Names:Os12g0570000, LOC_Os12g38210
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 12

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • cytosol Source: UniProtKB
  • nucleus Source: UniProtKB

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Lesion mimic (spontaneous cell death) phenotype. Expression of defense-related genes and enhanced non-race-specific resistance to rice blast fungus (M.oryzea) and to bacterial blight (X.oryzae pv oryzae). Delay in flowering time under long day (LD) conditions.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi290V → R: Loss of E3 ubiquitin ligase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000721271 – 694E3 ubiquitin-protein ligase SPL11Add BLAST694

Proteomic databases

PaxDbiQ0IMG9.
PRIDEiQ0IMG9.

Expressioni

Tissue specificityi

Highly expressed in leaf, at intermediate levels in shoot and weakly in root.1 Publication

Inductioni

In both incompatible and compatible interactions with rice blast fungus (M.grisea).

Gene expression databases

GenevisibleiQ0IMG9. OS.

Interactioni

Subunit structurei

Interacts with SPIN1 (via N-terminus).1 Publication

Protein-protein interaction databases

STRINGi39947.LOC_Os12g38210.1.

Structurei

3D structure databases

ProteinModelPortaliQ0IMG9.
SMRiQ0IMG9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini272 – 346U-boxAdd BLAST75
Repeati398 – 438ARM 1Add BLAST41
Repeati439 – 479ARM 2Add BLAST41
Repeati480 – 520ARM 3Add BLAST41
Repeati521 – 561ARM 4Add BLAST41
Repeati562 – 602ARM 5Add BLAST41
Repeati603 – 650ARM 6Add BLAST48

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi7 – 12Poly-Glu6
Compositional biasi18 – 42Ala-richAdd BLAST25
Compositional biasi77 – 84Poly-Glu8

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410ITM3. Eukaryota.
ENOG410Y3P9. LUCA.
InParanoidiQ0IMG9.
OrthoDBiEOG093607HO.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR003613. Ubox_domain.
IPR013083. Znf_RING/FYVE/PHD.
PfamiView protein in Pfam
PF00514. Arm. 3 hits.
PF04564. U-box. 1 hit.
SMARTiView protein in SMART
SM00185. ARM. 5 hits.
SM00504. Ubox. 1 hit.
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiView protein in PROSITE
PS50176. ARM_REPEAT. 1 hit.
PS51698. U_BOX. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0IMG9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGDRAEEEE GEAPPPEARA AAAVERVAAA VEAVAAGAGA GAGEYRNAYR
60 70 80 90 100
RQLLALSRRI RLLGPFVEEL RERRRGEGEG EEEERALAPL ADALEAALAL
110 120 130 140 150
LRLGREGSRI SLVLERDSVM KKFQGVILQL EQALCDIPYN ELDISDEVRE
160 170 180 190 200
QVELVHAQLK RAKERIDMPD DEFYNDLLSV YDKNYDPSAE LAILGRLSEK
210 220 230 240 250
LHLMTITDLT QESLALHEMV ASGGGQDPGE HIERMSMLLK KIKDFVQTQN
260 270 280 290 300
PDMGPPMASR VLDSNGDSRP ITIPDEFRCP ISLELMKDPV IVSTGQTYER
310 320 330 340 350
ACIEKWIASG HHTCPTTQQK MSTSALTPNY VLRSLISQWC ETNGMEPPKR
360 370 380 390 400
STQPNKPTPA CSSSERANID ALLSKLCSPD TEEQRSAAAE LRLLAKRNAN
410 420 430 440 450
NRICIAEAGA IPLLLSLLSS SDLRTQEHAV TALLNLSIHE DNKASIISSG
460 470 480 490 500
AVPSIVHVLK NGSMEARENA AATLFSLSVI DEYKVTIGGM GAIPALVVLL
510 520 530 540 550
GEGSQRGKKD AAAALFNLCI YQGNKGRAIR AGLVPLIMGL VTNPTGALMD
560 570 580 590 600
EAMAILSILS SHPEGKAAIG AAEPVPVLVE MIGSGTPRNR ENAAAVMLHL
610 620 630 640 650
CSGEHHLVHL ARAQECGIMV PLRELALNGT DRGKRKAVQL LERMSRFLVQ
660 670 680 690
QQEEQESQSQ ASAQVPPQAT PEQVPENDIP EQLDSPASQY PMVV
Length:694
Mass (Da):75,300
Last modified:September 11, 2007 - v2
Checksum:i3FEDB892C7289E05
GO

Sequence cautioni

The sequence ABA99652 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAF30096 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY652589 mRNA. Translation: AAT94160.1.
AY652590 Genomic DNA. Translation: AAT94161.1.
DP000011 Genomic DNA. Translation: ABA99652.1. Sequence problems.
AP008218 Genomic DNA. Translation: BAF30096.2. Sequence problems.
AP014968 Genomic DNA. No translation available.
AK105835 mRNA. No translation available.
UniGeneiOs.21174.

Similar proteinsi

Entry informationi

Entry nameiSL11_ORYSJ
AccessioniPrimary (citable) accession number: Q0IMG9
Secondary accession number(s): Q2QND2, Q64HA9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: August 30, 2017
This is version 77 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations