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Protein

E3 ubiquitin-protein ligase SPL11

Gene

SPL11

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that negatively regulates programmed cell death and disease resistance. Participates in flowering time control by mediating ubiquitination and subsequent proteasomal degradation of SPIN1.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  • defense response Source: UniProtKB-KW
  • flower development Source: UniProtKB-KW
  • negative regulation of defense response Source: UniProtKB
  • negative regulation of programmed cell death Source: UniProtKB
  • protein ubiquitination Source: UniProtKB
  • regulation of long-day photoperiodism, flowering Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Flowering, Plant defense, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase SPL11 (EC:6.3.2.-)
Alternative name(s):
Cell death-related protein SPL11
Protein spotted leaf 11
Gene namesi
Name:SPL11
Ordered Locus Names:Os12g0570000, LOC_Os12g38210
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 12

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • cytosol Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Lesion mimic (spontaneous cell death) phenotype. Expression of defense-related genes and enhanced non-race-specific resistance to rice blast fungus (M.oryzea) and to bacterial blight (X.oryzae pv oryzae). Delay in flowering time under long day (LD) conditions.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi290 – 2901V → R: Loss of E3 ubiquitin ligase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 694694E3 ubiquitin-protein ligase SPL11PRO_0000072127Add
BLAST

Proteomic databases

PaxDbiQ0IMG9.

Expressioni

Tissue specificityi

Highly expressed in leaf, at intermediate levels in shoot and weakly in root.1 Publication

Inductioni

In both incompatible and compatible interactions with rice blast fungus (M.grisea).

Gene expression databases

GenevisibleiQ0IMG9. OS.

Interactioni

Subunit structurei

Interacts with SPIN1 (via N-terminus).1 Publication

Protein-protein interaction databases

STRINGi39947.LOC_Os12g38210.1.

Structurei

3D structure databases

ProteinModelPortaliQ0IMG9.
SMRiQ0IMG9. Positions 274-346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini272 – 34675U-boxAdd
BLAST
Repeati398 – 43841ARM 1Add
BLAST
Repeati439 – 47941ARM 2Add
BLAST
Repeati480 – 52041ARM 3Add
BLAST
Repeati521 – 56141ARM 4Add
BLAST
Repeati562 – 60241ARM 5Add
BLAST
Repeati603 – 65048ARM 6Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi7 – 126Poly-Glu
Compositional biasi18 – 4225Ala-richAdd
BLAST
Compositional biasi77 – 848Poly-Glu

Sequence similaritiesi

Contains 6 ARM repeats.PROSITE-ProRule annotation
Contains 1 U-box domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410ITM3. Eukaryota.
ENOG410Y3P9. LUCA.
InParanoidiQ0IMG9.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR003613. Ubox_domain.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00514. Arm. 3 hits.
PF04564. U-box. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 5 hits.
SM00504. Ubox. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 1 hit.
PS51698. U_BOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0IMG9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGDRAEEEE GEAPPPEARA AAAVERVAAA VEAVAAGAGA GAGEYRNAYR
60 70 80 90 100
RQLLALSRRI RLLGPFVEEL RERRRGEGEG EEEERALAPL ADALEAALAL
110 120 130 140 150
LRLGREGSRI SLVLERDSVM KKFQGVILQL EQALCDIPYN ELDISDEVRE
160 170 180 190 200
QVELVHAQLK RAKERIDMPD DEFYNDLLSV YDKNYDPSAE LAILGRLSEK
210 220 230 240 250
LHLMTITDLT QESLALHEMV ASGGGQDPGE HIERMSMLLK KIKDFVQTQN
260 270 280 290 300
PDMGPPMASR VLDSNGDSRP ITIPDEFRCP ISLELMKDPV IVSTGQTYER
310 320 330 340 350
ACIEKWIASG HHTCPTTQQK MSTSALTPNY VLRSLISQWC ETNGMEPPKR
360 370 380 390 400
STQPNKPTPA CSSSERANID ALLSKLCSPD TEEQRSAAAE LRLLAKRNAN
410 420 430 440 450
NRICIAEAGA IPLLLSLLSS SDLRTQEHAV TALLNLSIHE DNKASIISSG
460 470 480 490 500
AVPSIVHVLK NGSMEARENA AATLFSLSVI DEYKVTIGGM GAIPALVVLL
510 520 530 540 550
GEGSQRGKKD AAAALFNLCI YQGNKGRAIR AGLVPLIMGL VTNPTGALMD
560 570 580 590 600
EAMAILSILS SHPEGKAAIG AAEPVPVLVE MIGSGTPRNR ENAAAVMLHL
610 620 630 640 650
CSGEHHLVHL ARAQECGIMV PLRELALNGT DRGKRKAVQL LERMSRFLVQ
660 670 680 690
QQEEQESQSQ ASAQVPPQAT PEQVPENDIP EQLDSPASQY PMVV
Length:694
Mass (Da):75,300
Last modified:September 11, 2007 - v2
Checksum:i3FEDB892C7289E05
GO

Sequence cautioni

The sequence ABA99652.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence BAF30096.2 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY652589 mRNA. Translation: AAT94160.1.
AY652590 Genomic DNA. Translation: AAT94161.1.
DP000011 Genomic DNA. Translation: ABA99652.1. Sequence problems.
AP008218 Genomic DNA. Translation: BAF30096.2. Sequence problems.
AP014968 Genomic DNA. No translation available.
AK105835 mRNA. No translation available.
UniGeneiOs.21174.

Genome annotation databases

KEGGiosa:4352575.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY652589 mRNA. Translation: AAT94160.1.
AY652590 Genomic DNA. Translation: AAT94161.1.
DP000011 Genomic DNA. Translation: ABA99652.1. Sequence problems.
AP008218 Genomic DNA. Translation: BAF30096.2. Sequence problems.
AP014968 Genomic DNA. No translation available.
AK105835 mRNA. No translation available.
UniGeneiOs.21174.

3D structure databases

ProteinModelPortaliQ0IMG9.
SMRiQ0IMG9. Positions 274-346.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os12g38210.1.

Proteomic databases

PaxDbiQ0IMG9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiosa:4352575.

Phylogenomic databases

eggNOGiENOG410ITM3. Eukaryota.
ENOG410Y3P9. LUCA.
InParanoidiQ0IMG9.

Enzyme and pathway databases

UniPathwayiUPA00143.

Gene expression databases

GenevisibleiQ0IMG9. OS.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR003613. Ubox_domain.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00514. Arm. 3 hits.
PF04564. U-box. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 5 hits.
SM00504. Ubox. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 1 hit.
PS51698. U_BOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Spotted leaf11, a negative regulator of plant cell death and defense, encodes a U-Box/Armadillo repeat protein endowed with E3 Ubiquitin ligase activity."
    Zeng L.-R., Qu S., Bordeos A., Yang C., Baraoidan M., Yan H., Xie Q., Nahm B.H., Leung H., Wang G.-L.
    Plant Cell 16:2795-2808(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, MUTAGENESIS OF VAL-290.
    Strain: cv. Nipponbare.
  2. "The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications."
    The rice chromosomes 11 and 12 sequencing consortia
    BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  5. Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  6. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 178-694.
    Strain: cv. Nipponbare.
  7. "SPIN1, a K homology domain protein negatively regulated and ubiquitinated by the E3 ubiquitin ligase SPL11, is involved in flowering time control in rice."
    Vega-Sanchez M.E., Zeng L., Chen S., Leung H., Wang G.L.
    Plant Cell 20:1456-1469(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SPIN1, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSL11_ORYSJ
AccessioniPrimary (citable) accession number: Q0IMG9
Secondary accession number(s): Q2QND2, Q64HA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: June 8, 2016
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.