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Protein

ATP-dependent RNA helicase DDX1

Gene

DDX1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB: together with archease (ZBTB8OS), acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi46 – 53ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Exonuclease, Helicase, Hydrolase, Nuclease, RNA-binding
Biological processmRNA processing, Transcription, Transcription regulation, tRNA processing
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DDX1 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 1
Gene namesi
Name:DDX1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 11

Organism-specific databases

VGNCiVGNC:27955 DDX1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002840751 – 740ATP-dependent RNA helicase DDX1Add BLAST740

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei239N6-acetyllysineBy similarity1
Modified residuei268N6-acetyllysineBy similarity1
Modified residuei281N6-acetyllysine; alternateBy similarity1
Cross-linki281Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei481PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ0IIK5
PeptideAtlasiQ0IIK5
PRIDEiQ0IIK5

Expressioni

Gene expression databases

BgeeiENSBTAG00000009906

Interactioni

Subunit structurei

Interacts with FAM98A (via N- and C-terminus). Interacts with PHF5A (via C-terminus). Interacts with MBNL1. Interacts with CSTF2. Interacts with HNRNPK. Interacts with ATM. Interacts with RELA (via C-terminus). Component of the tRNA-splicing ligase complex. Interacts with PQBP1.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013075

Structurei

3D structure databases

ProteinModelPortaliQ0IIK5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 428Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST427
Domaini70 – 247B30.2/SPRYPROSITE-ProRule annotationAdd BLAST178
Domaini493 – 681Helicase C-terminalPROSITE-ProRule annotationAdd BLAST189

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 525Necessary for interaction with RELABy similarityAdd BLAST525
Regioni1 – 295Necessary for interaction with HNRNPKBy similarityAdd BLAST295
Regioni525 – 740Necessary for interaction with HNRNPKBy similarityAdd BLAST216

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi370 – 373DEAD box4

Domaini

The helicase domain is involved in the stimulation of RELA transcriptional activity.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0349 Eukaryota
COG0513 LUCA
GeneTreeiENSGT00910000144265
HOVERGENiHBG005462
InParanoidiQ0IIK5
KOiK13177
OMAiTLNNVKQ
OrthoDBiEOG091G03WJ
TreeFamiTF106114

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR013320 ConA-like_dom_sf
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR014014 RNA_helicase_DEAD_Q_motif
IPR003877 SPRY_dom
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
PF00622 SPRY, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
SSF52540 SSF52540, 3 hits
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS51192 HELICASE_ATP_BIND_1, 2 hits
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequencei

Sequence statusi: Complete.

Q0IIK5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAFSEMGVM PEIAQAVEEM DWLLPTDIQA ESIPLILGGG DVLMAAETGS
60 70 80 90 100
GKTGAFSIPV IQIVYETLKD QQEGKKGKAT IKTGASVLNK WQMNPYDRGS
110 120 130 140 150
AFAIGSDGLC CQSREVKEWH GCRATKGLTK GKHYYEVSCH DQGLCRVGWS
160 170 180 190 200
SMQASLDLGT DKFGFGFGGT GKKSHNKQFD NYGEEFTMHD TIGCYLDIDK
210 220 230 240 250
GHVKFSKNGK DLGLAFEIPP HMKNQALFPA CVLKNAELKF NFGEEEFKFP
260 270 280 290 300
PKDGFVALSK APESFVVKSQ HTGSAQVAQT KFLPNAPKAL IVEPSRELAE
310 320 330 340 350
QTLNNVKQFK KYIDNPKLRE LLIIGGVAAR DQLSVLDNGV DIVVGTPGRL
360 370 380 390 400
DDLVSTGKLN LSQVRFLVLD EADGLLSQGY SDFINRIHNQ IPQITSDGKR
410 420 430 440 450
LQVIVCSATL HSFDVKKLSE KIMHFPTWVD LKGEDSVPDT VHHVVVPVNP
460 470 480 490 500
KTDRLWERLG KSHIRTDEVH AKDNTRPGAN SPEMWSEAIK ILKGEYAVRA
510 520 530 540 550
IKEHKMDQAI IFCRTKIDCD NLEQYFMQQG GGPDKKGHQF SCVCLHGDRK
560 570 580 590 600
PHERKQNLER FKKGDVRFLI CTDVAARGID IHGVPYVINV TLPDEKQNYV
610 620 630 640 650
HRIGRVGRAE RMGLAISLVA TEKEKVWYHV CSSRGKGCYN TRLKEDGGCT
660 670 680 690 700
IWYNEMQLLS EIEEHLNCTI SQVEPDIKVP VDEFDGKVTY GQKRTLGGGN
710 720 730 740
YKGHVDVLAP TVQELAALEK EAQTSFLHLG YLPNQLFRTF
Length:740
Mass (Da):82,414
Last modified:October 3, 2006 - v1
Checksum:i48D7C8E4801479A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC122599 mRNA Translation: AAI22600.1
RefSeqiNP_001068936.1, NM_001075468.1
UniGeneiBt.1784

Genome annotation databases

EnsembliENSBTAT00000013075; ENSBTAP00000013075; ENSBTAG00000009906
GeneIDi510816
KEGGibta:510816

Similar proteinsi

Entry informationi

Entry nameiDDX1_BOVIN
AccessioniPrimary (citable) accession number: Q0IIK5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: October 3, 2006
Last modified: March 28, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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