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Protein

DnaJ homolog subfamily B member 6

Gene

DNAJB6

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins (By similarity). Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity (By similarity).By similarity1 Publication

Keywords - Molecular functioni

Chaperone

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily B member 6
Alternative name(s):
MRJ
Gene namesi
Name:DNAJB6
Synonyms:MRJ
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 4

Subcellular locationi

  • Cytoplasmperinuclear region By similarity
  • Nucleus By similarity
  • CytoplasmmyofibrilsarcomereZ line By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 242241DnaJ homolog subfamily B member 6PRO_0000290022Add
BLAST

Proteomic databases

PRIDEiQ0III6.

Expressioni

Tissue specificityi

Expressed in all tissues examined with highest expression in brain and retina and lower levels observed in testis, spleen, heart, liver and kidney.1 Publication

Gene expression databases

BgeeiENSBTAG00000000771.
ExpressionAtlasiQ0III6. baseline.

Interactioni

Subunit structurei

Homooligomer. Interacts with BAG3, HSPB8 and STUB1. Interacts with HSP70, KRT18 and PTTG. Interacts with ALKBH1. Interacts with histone deacetylases HDAC4, HDAC6, and SIRT2, HDAC activity is required for antiaggregation.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ0III6.
SMRiQ0III6. Positions 1-69.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 6968JPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 146145Interaction with HSP70By similarityAdd
BLAST
Regioni119 – 242124Interaction with KRT18By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi75 – 806Poly-Gly
Compositional biasi83 – 17290Gly/Phe-richAdd
BLAST
Compositional biasi83 – 16482Phe-richAdd
BLAST
Compositional biasi155 – 19440Ser-richAdd
BLAST
Compositional biasi176 – 1794Poly-Ser

Domaini

The antiaggregation activity of resides in the serine-rich region and the C-terminus.By similarity

Sequence similaritiesi

Contains 1 J domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00760000118947.
HOGENOMiHOG000111538.
HOVERGENiHBG066998.
InParanoidiQ0III6.
KOiK09512.
OMAiFFMPTSG.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0III6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDYYEVLGV QRHASAEDIK KAYRKLALKW HPDKNPENKE EAERKFKQVA
60 70 80 90 100
EAYEVLSDAK KRDIYDRYGK EGLNGGGGGG SHFDSPFEFG FTFRNPEDVF
110 120 130 140 150
REFFGGRDPF SFDFFEDPFE DFFGHRRGPR GSRSRGTGSF FSTFSGFPSF
160 170 180 190 200
GGAFPSFDAG FSSFGSLGHG GLTAFSSSSA FGGSGMGNYK SISTSTKVVN
210 220 230 240
GRKITTKRIV ENGQERVEVE EDGQLKSLTI NGKEQLLRLD NK
Length:242
Mass (Da):26,944
Last modified:October 3, 2006 - v1
Checksum:i97A0BF6B48B4A704
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti125 – 1251H → N in AAL73393 (PubMed:11896048).Curated
Sequence conflicti152 – 1532GA → SL in AAL73393 (PubMed:11896048).Curated
Sequence conflicti201 – 2011G → S in AAL73393 (PubMed:11896048).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF426743 mRNA. Translation: AAL73393.1.
BC122622 mRNA. Translation: AAI22623.1.
RefSeqiNP_776957.2. NM_174532.3.
UniGeneiBt.1731.

Genome annotation databases

EnsembliENSBTAT00000001018; ENSBTAP00000001018; ENSBTAG00000000771.
GeneIDi282215.
KEGGibta:282215.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF426743 mRNA. Translation: AAL73393.1.
BC122622 mRNA. Translation: AAI22623.1.
RefSeqiNP_776957.2. NM_174532.3.
UniGeneiBt.1731.

3D structure databases

ProteinModelPortaliQ0III6.
SMRiQ0III6. Positions 1-69.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ0III6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000001018; ENSBTAP00000001018; ENSBTAG00000000771.
GeneIDi282215.
KEGGibta:282215.

Organism-specific databases

CTDi10049.

Phylogenomic databases

GeneTreeiENSGT00760000118947.
HOGENOMiHOG000111538.
HOVERGENiHBG066998.
InParanoidiQ0III6.
KOiK09512.
OMAiFFMPTSG.

Gene expression databases

BgeeiENSBTAG00000000771.
ExpressionAtlasiQ0III6. baseline.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNJB6_BOVIN
AccessioniPrimary (citable) accession number: Q0III6
Secondary accession number(s): Q8WN90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 12, 2007
Last sequence update: October 3, 2006
Last modified: September 7, 2016
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.