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Protein

DnaJ homolog subfamily B member 6

Gene

DNAJB6

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins (By similarity). Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity (By similarity).By similarity1 Publication

Keywordsi

Molecular functionChaperone

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily B member 6
Alternative name(s):
MRJ
Gene namesi
Name:DNAJB6
Synonyms:MRJ
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002900221 – 242DnaJ homolog subfamily B member 6Add BLAST242

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei135Omega-N-methylarginineBy similarity1

Keywords - PTMi

Methylation

Expressioni

Tissue specificityi

Expressed in all tissues examined with highest expression in brain and retina and lower levels observed in testis, spleen, heart, liver and kidney.1 Publication

Gene expression databases

BgeeiENSBTAG00000000771.
ExpressionAtlasiQ0III6. baseline.

Interactioni

Subunit structurei

Homooligomer. Interacts with BAG3, HSPB8 and STUB1. Interacts with HSP70, KRT18 and PTTG. Interacts with ALKBH1. Interacts with histone deacetylases HDAC4, HDAC6, and SIRT2, HDAC activity is required for antiaggregation.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000033245.

Structurei

3D structure databases

ProteinModelPortaliQ0III6.
SMRiQ0III6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 69JPROSITE-ProRule annotationAdd BLAST68

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 146Interaction with HSP70By similarityAdd BLAST146
Regioni119 – 242Interaction with KRT18By similarityAdd BLAST124

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi75 – 80Poly-Gly6
Compositional biasi83 – 172Gly/Phe-richAdd BLAST90
Compositional biasi83 – 164Phe-richAdd BLAST82
Compositional biasi155 – 194Ser-richAdd BLAST40
Compositional biasi176 – 179Poly-Ser4

Domaini

The antiaggregation activity of resides in the serine-rich region and the C-terminus.By similarity

Phylogenomic databases

GeneTreeiENSGT00760000118947.
HOGENOMiHOG000111538.
HOVERGENiHBG066998.
InParanoidiQ0III6.
KOiK09512.
OMAiFGAPMLN.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
InterProiView protein in InterPro
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
PfamiView protein in Pfam
PF00226. DnaJ. 1 hit.
PRINTSiPR00625. JDOMAIN.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiView protein in PROSITE
PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0III6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDYYEVLGV QRHASAEDIK KAYRKLALKW HPDKNPENKE EAERKFKQVA
60 70 80 90 100
EAYEVLSDAK KRDIYDRYGK EGLNGGGGGG SHFDSPFEFG FTFRNPEDVF
110 120 130 140 150
REFFGGRDPF SFDFFEDPFE DFFGHRRGPR GSRSRGTGSF FSTFSGFPSF
160 170 180 190 200
GGAFPSFDAG FSSFGSLGHG GLTAFSSSSA FGGSGMGNYK SISTSTKVVN
210 220 230 240
GRKITTKRIV ENGQERVEVE EDGQLKSLTI NGKEQLLRLD NK
Length:242
Mass (Da):26,944
Last modified:October 3, 2006 - v1
Checksum:i97A0BF6B48B4A704
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti125H → N in AAL73393 (PubMed:11896048).Curated1
Sequence conflicti152 – 153GA → SL in AAL73393 (PubMed:11896048).Curated2
Sequence conflicti201G → S in AAL73393 (PubMed:11896048).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF426743 mRNA. Translation: AAL73393.1.
BC122622 mRNA. Translation: AAI22623.1.
RefSeqiNP_776957.2. NM_174532.3.
UniGeneiBt.1731.

Genome annotation databases

EnsembliENSBTAT00000001018; ENSBTAP00000001018; ENSBTAG00000000771.
GeneIDi282215.
KEGGibta:282215.

Similar proteinsi

Entry informationi

Entry nameiDNJB6_BOVIN
AccessioniPrimary (citable) accession number: Q0III6
Secondary accession number(s): Q8WN90
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 12, 2007
Last sequence update: October 3, 2006
Last modified: August 30, 2017
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome