Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Membrane-bound transcription factor site-2 protease

Gene

MBTPS2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Intramembrane proteolysis of sterol-regulatory element-binding proteins (SREBPs) within the first transmembrane segment thereby releasing the N-terminal segment with a portion of the transmembrane segment attached. Site-2 cleavage comes after site-1 cleavage which takes place in the lumenal loop (By similarity).By similarity

Catalytic activityi

Cleaves several transcription factors that are type-2 transmembrane proteins within membrane-spanning domains. Known substrates include sterol regulatory element-binding protein (SREBP) -1, SREBP-2 and forms of the transcriptional activator ATF6. SREBP-2 is cleaved at the site 477-DRSRILL-|-CVLTFLCLSFNPLTSLLQWGGA-505. The residues Asn-Pro, 11 residues distal to the site of cleavage in the membrane-spanning domain, are important for cleavage by S2P endopeptidase. Replacement of either of these residues does not prevent cleavage, but there is no cleavage if both of these residues are replaced.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi168Zinc; catalyticPROSITE-ProRule annotation1
Active sitei169PROSITE-ProRule annotation1
Metal bindingi172Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-BTA-1655829. Regulation of cholesterol biosynthesis by SREBP (SREBF).
R-BTA-174800. Chylomicron-mediated lipid transport.
R-BTA-381033. ATF6 (ATF6-alpha) activates chaperones.

Protein family/group databases

MEROPSiM50.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-bound transcription factor site-2 protease (EC:3.4.24.85)
Alternative name(s):
Endopeptidase S2P
Gene namesi
Name:MBTPS2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome X

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3CytoplasmicBy similarity3
Transmembranei4 – 24HelicalSequence analysisAdd BLAST21
Topological domaini25 – 74LumenalBy similarityAdd BLAST50
Transmembranei75 – 95HelicalSequence analysisAdd BLAST21
Transmembranei96 – 107HelicalSequence analysisAdd BLAST12
Topological domaini108 – 141LumenalBy similarityAdd BLAST34
Transmembranei142 – 166HelicalSequence analysisAdd BLAST25
Transmembranei171 – 183HelicalSequence analysisAdd BLAST13
Transmembranei184 – 206HelicalSequence analysisAdd BLAST23
Transmembranei226 – 248HelicalSequence analysisAdd BLAST23
Topological domaini249 – 443LumenalBy similarityAdd BLAST195
Transmembranei444 – 461HelicalSequence analysisAdd BLAST18
Transmembranei462 – 473HelicalSequence analysisAdd BLAST12
Topological domaini474 – 489LumenalSequence analysisAdd BLAST16
Transmembranei490 – 510HelicalSequence analysisAdd BLAST21
Topological domaini511 – 516CytoplasmicSequence analysis6

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002615911 – 516Membrane-bound transcription factor site-2 proteaseAdd BLAST516

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi334N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ0III2.
PRIDEiQ0III2.

Expressioni

Gene expression databases

BgeeiENSBTAG00000008183.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000010764.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi109 – 133Poly-SerAdd BLAST25
Compositional biasi282 – 383Cys-richAdd BLAST102
Compositional biasi377 – 381Poly-Ser5

Sequence similaritiesi

Belongs to the peptidase M50A family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2921. Eukaryota.
COG0750. LUCA.
GeneTreeiENSGT00510000048066.
HOGENOMiHOG000231255.
HOVERGENiHBG006397.
InParanoidiQ0III2.
KOiK07765.
OMAiTELSHDH.
OrthoDBiEOG091G05YC.
TreeFamiTF314478.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001193. MBTPS2.
IPR001478. PDZ.
IPR008915. Peptidase_M50.
[Graphical view]
PANTHERiPTHR13325. PTHR13325. 1 hit.
PfamiPF02163. Peptidase_M50. 1 hit.
[Graphical view]
PRINTSiPR01000. SREBPS2PTASE.
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0III2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPVSLVVVV VGGWTAVYLT DLVLKSSVYF KHSYEDWLEN NGLSISPFHI
60 70 80 90 100
RWQTAVFNRA FYSWGRRKAR MLYQWFNFGM VFGVIAMFSS FFLLGKTLIQ
110 120 130 140 150
TLGQMMADSS YSSSSSSSSH SSSSSSSSSS SSSLYNEQVL QVVVPGINLP
160 170 180 190 200
VNQLTYFFAA VLISGVVHEI GHGIAAIREQ VRFNGFGIFL FIIYPGAFVD
210 220 230 240 250
LFTTHLQLIS PVQQLRIFCA GIWHNFILAL LGILALILLP VILLPFYYTG
260 270 280 290 300
VGVLITEVAE DSPAIGPRGL FVGDLVTHLQ DCPVTNVQDW NECLDTITYE
310 320 330 340 350
PQIGYCISAS TLQQLSFPVR AYKRLDGSTE CCNNHSLTDV CFSYRNNFNK
360 370 380 390 400
RLHTCLPARK AVEATQVCRT NKDCKKSSSS SFCIIPSLET HTRLIKVKHP
410 420 430 440 450
PQIDMLYVGH PLHLHYTVSI TSFIPRFKFL SIDLPVVVET FVKYLISLSG
460 470 480 490 500
ALAIVNAVPC FALDGQWILN SFLDATLTSV IGDNDVKDLI GFFILLGGSI
510
LLAANVALGL WMVTAR
Length:516
Mass (Da):57,214
Last modified:October 3, 2006 - v1
Checksum:iF2E18EC52C644074
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC122628 mRNA. Translation: AAI22629.1.
RefSeqiNP_001069449.1. NM_001075981.1.
UniGeneiBt.38187.

Genome annotation databases

EnsembliENSBTAT00000010764; ENSBTAP00000010764; ENSBTAG00000008183.
GeneIDi533134.
KEGGibta:533134.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC122628 mRNA. Translation: AAI22629.1.
RefSeqiNP_001069449.1. NM_001075981.1.
UniGeneiBt.38187.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000010764.

Protein family/group databases

MEROPSiM50.001.

Proteomic databases

PaxDbiQ0III2.
PRIDEiQ0III2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000010764; ENSBTAP00000010764; ENSBTAG00000008183.
GeneIDi533134.
KEGGibta:533134.

Organism-specific databases

CTDi51360.

Phylogenomic databases

eggNOGiKOG2921. Eukaryota.
COG0750. LUCA.
GeneTreeiENSGT00510000048066.
HOGENOMiHOG000231255.
HOVERGENiHBG006397.
InParanoidiQ0III2.
KOiK07765.
OMAiTELSHDH.
OrthoDBiEOG091G05YC.
TreeFamiTF314478.

Enzyme and pathway databases

ReactomeiR-BTA-1655829. Regulation of cholesterol biosynthesis by SREBP (SREBF).
R-BTA-174800. Chylomicron-mediated lipid transport.
R-BTA-381033. ATF6 (ATF6-alpha) activates chaperones.

Gene expression databases

BgeeiENSBTAG00000008183.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001193. MBTPS2.
IPR001478. PDZ.
IPR008915. Peptidase_M50.
[Graphical view]
PANTHERiPTHR13325. PTHR13325. 1 hit.
PfamiPF02163. Peptidase_M50. 1 hit.
[Graphical view]
PRINTSiPR01000. SREBPS2PTASE.
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMBTP2_BOVIN
AccessioniPrimary (citable) accession number: Q0III2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: October 3, 2006
Last modified: November 30, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.