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Q0IIG5

- PFKAM_BOVIN

UniProt

Q0IIG5 - PFKAM_BOVIN

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Protein
ATP-dependent 6-phosphofructokinase, muscle type
Gene
PFKM
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei24 – 241ATP; via amide nitrogen By similarity
Metal bindingi118 – 1181Magnesium; catalytic By similarity
Active sitei165 – 1651Proton acceptor By similarity
Binding sitei200 – 2001Substrate; shared with dimeric partner By similarity
Binding sitei263 – 2631Substrate By similarity
Binding sitei291 – 2911Substrate; shared with dimeric partner By similarity
Binding sitei470 – 4701Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei565 – 5651Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei628 – 6281Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei654 – 6541Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei734 – 7341Allosteric activator fructose 2,6-bisphosphate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi87 – 882ATP By similarity
Nucleotide bindingi117 – 1204ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB
  2. ATP binding Source: UniProtKB-KW
  3. fructose binding Source: Ensembl
  4. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. carbohydrate phosphorylation Source: GOC
  2. fructose 6-phosphate metabolic process Source: InterPro
  3. glucose homeostasis Source: Ensembl
  4. glycolytic process Source: UniProtKB-UniPathway
  5. muscle cell cellular homeostasis Source: Ensembl
  6. positive regulation of insulin secretion Source: Ensembl
  7. protein oligomerization Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_224187. Glycolysis.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase, muscle type (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
PFK-M
Alternative name(s):
6-phosphofructokinase type A
Phosphofructo-1-kinase isozyme A
Short name:
PFK-A
Phosphohexokinase
Gene namesi
Name:PFKM
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 5

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
  2. apical plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 779778ATP-dependent 6-phosphofructokinase, muscle typeUniRule annotation
PRO_0000284438Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonine By similarity
Glycosylationi529 – 5291O-linked (GlcNAc) By similarity
Modified residuei666 – 6661Phosphoserine By similarity
Modified residuei774 – 7741Phosphoserine By similarity

Post-translational modificationi

GlcNAcylation decreases enzyme activity By similarity.UniRule annotation

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ0IIG5.
PRIDEiQ0IIG5.

Interactioni

Subunit structurei

Homo- and heterotetramers By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000000358.

Structurei

3D structure databases

ProteinModelPortaliQ0IIG5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 389388N-terminal catalytic PFK domain 1UniRule annotation
Add
BLAST
Regioni163 – 1653Substrate binding By similarity
Regioni207 – 2093Substrate binding By similarity
Regioni297 – 3004Substrate binding By similarity
Regioni390 – 40011Interdomain linkerUniRule annotation
Add
BLAST
Regioni401 – 779379C-terminal regulatory PFK domain 2UniRule annotation
Add
BLAST
Regioni527 – 5315Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni572 – 5743Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni660 – 6634Allosteric activator fructose 2,6-bisphosphate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
GeneTreeiENSGT00390000013209.
HOGENOMiHOG000200154.
HOVERGENiHBG000976.
InParanoidiQ0IIG5.
KOiK00850.
OMAiVYHMASK.
OrthoDBiEOG7ZSHV5.
TreeFamiTF300411.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0IIG5-1 [UniParc]FASTAAdd to Basket

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MTHEHHAAKT LGIGKAIAVL TSGGDAQGMN AAVRAVVRVG IYTGARVFFV    50
HEGYQGLVDG GDNIREATWE SVSMMLQLGG TVIGSARCKD FREREGRLRA 100
AHNLVKRGIT NLCVIGGDGS LTGADTFRSE WSDLLSDLQK SGKITAEEAT 150
KSSYLNIVGL VGSIDNDFCG TDMTIGTDSA LHRIIEIVDA ITTTAQSHQR 200
TFVLEVMGRH CGYLALVTSL SCGADWVFIP ECPPDDDWEE HLCRRLSETR 250
NRGSRLNIII VAEGAIDKNG KPITSEDIKN LVVKRLGYDT RVTVLGHVQR 300
GGTPSAFDRI LGSRMGVEAV MALLEGTPDT PACVVSLSGN QAVRLPLMEC 350
VQVTKDVTRA MDERRFDEAL KLRGRSFMNN WEVYKLLAHV RPPKSKSGSH 400
TVAVMNVGAP AAGMNAAVRS TVRIGLIQGN RMLVVHDGFE GLAKGQIEEA 450
GWSYVGGWTG QGGSKLGTKR TLPKKSFEQI SANITKFNIQ GLVIIGGFEA 500
YTGGLELMEG RKQYDELCIP FVVIPATVSN NVPGSDFSVG ADTALNTICM 550
TCDRIKQSAA GTKRRVFIIE TMGGYCGYLA TMAGLAAGAD AAYIFEDPFT 600
IRDLQVNVEH LVQKMKTTVK RGLVLRNEKC NENYTTDFIF NLYSEEGKGI 650
FDSRKNVLGH MQQGGSPTPF DRNFATKMGA KAMNWMSGKI KESYRNGRIF 700
ANSPDSGCVL GMRKRALVFQ PVTELKEQTD FEHRIPKEQW WLKLRPILKI 750
LAKYEIDLDT SEHAHLEHIS RKRSGEANV 779
Length:779
Mass (Da):85,294
Last modified:October 3, 2006 - v1
Checksum:iBBA2D207ADD079EE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC122655 mRNA. Translation: AAI22656.1.
RefSeqiNP_001068736.1. NM_001075268.1.
UniGeneiBt.61411.

Genome annotation databases

EnsembliENSBTAT00000000359; ENSBTAP00000000359; ENSBTAG00000000286.
GeneIDi506544.
KEGGibta:506544.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC122655 mRNA. Translation: AAI22656.1 .
RefSeqi NP_001068736.1. NM_001075268.1.
UniGenei Bt.61411.

3D structure databases

ProteinModelPortali Q0IIG5.
ModBasei Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000000358.

Proteomic databases

PaxDbi Q0IIG5.
PRIDEi Q0IIG5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000000359 ; ENSBTAP00000000359 ; ENSBTAG00000000286 .
GeneIDi 506544.
KEGGi bta:506544.

Organism-specific databases

CTDi 5213.

Phylogenomic databases

eggNOGi COG0205.
GeneTreei ENSGT00390000013209.
HOGENOMi HOG000200154.
HOVERGENi HBG000976.
InParanoidi Q0IIG5.
KOi K00850.
OMAi VYHMASK.
OrthoDBi EOG7ZSHV5.
TreeFami TF300411.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
Reactomei REACT_224187. Glycolysis.

Miscellaneous databases

NextBioi 20867650.

Family and domain databases

HAMAPi MF_03184. Phosphofructokinase_I_E.
InterProi IPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 2 hits.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Basal ganglia.

Entry informationi

Entry nameiPFKAM_BOVIN
AccessioniPrimary (citable) accession number: Q0IIG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: October 3, 2006
Last modified: September 3, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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