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Protein

ATP-dependent 6-phosphofructokinase, muscle type

Gene

PFKM

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei24 – 241ATP; via amide nitrogenUniRule annotation
Metal bindingi118 – 1181Magnesium; catalyticUniRule annotation
Active sitei165 – 1651Proton acceptorUniRule annotation
Binding sitei200 – 2001Substrate; shared with dimeric partnerUniRule annotation
Binding sitei263 – 2631SubstrateUniRule annotation
Binding sitei291 – 2911Substrate; shared with dimeric partnerUniRule annotation
Binding sitei470 – 4701Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei565 – 5651Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
Binding sitei628 – 6281Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei654 – 6541Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
Binding sitei734 – 7341Allosteric activator fructose 2,6-bisphosphateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi87 – 882ATPUniRule annotation
Nucleotide bindingi117 – 1204ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB
  2. ATP binding Source: UniProtKB-KW
  3. fructose binding Source: Ensembl
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate phosphorylation Source: GOC
  2. fructose 6-phosphate metabolic process Source: InterPro
  3. glucose homeostasis Source: Ensembl
  4. glycolytic process Source: UniProtKB-UniPathway
  5. muscle cell cellular homeostasis Source: Ensembl
  6. positive regulation of insulin secretion Source: Ensembl
  7. protein oligomerization Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_333435. Glycolysis.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase, muscle typeUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PFK-M
Alternative name(s):
6-phosphofructokinase type A
Phosphofructo-1-kinase isozyme A
Short name:
PFK-A
PhosphohexokinaseUniRule annotation
Gene namesi
Name:PFKM
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 5

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: Ensembl
  2. apical plasma membrane Source: Ensembl
  3. extracellular vesicular exosome Source: Ensembl
  4. sperm principal piece Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 779778ATP-dependent 6-phosphofructokinase, muscle typePRO_0000284438Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonineBy similarity
Glycosylationi529 – 5291O-linked (GlcNAc)By similarity
Modified residuei666 – 6661PhosphoserineBy similarity
Modified residuei774 – 7741PhosphoserineBy similarity

Post-translational modificationi

GlcNAcylation decreases enzyme activity.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ0IIG5.
PRIDEiQ0IIG5.

Interactioni

Subunit structurei

Homo- and heterotetramers. Interacts (via C-terminus) with HK1 (via N-terminal spermatogenic cell-specific region) (By similarity).UniRule annotationBy similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000000358.

Structurei

3D structure databases

ProteinModelPortaliQ0IIG5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 389388N-terminal catalytic PFK domain 1Add
BLAST
Regioni163 – 1653Substrate bindingUniRule annotation
Regioni207 – 2093Substrate bindingUniRule annotation
Regioni297 – 3004Substrate bindingUniRule annotation
Regioni390 – 40011Interdomain linkerAdd
BLAST
Regioni401 – 779379C-terminal regulatory PFK domain 2Add
BLAST
Regioni527 – 5315Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni572 – 5743Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni660 – 6634Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
GeneTreeiENSGT00390000013209.
HOGENOMiHOG000200154.
HOVERGENiHBG000976.
InParanoidiQ0IIG5.
KOiK00850.
OMAiVYHMASK.
OrthoDBiEOG7ZSHV5.
TreeFamiTF300411.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-Pfructokinase_euk.
IPR022953. ATP_PFK.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0IIG5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHEHHAAKT LGIGKAIAVL TSGGDAQGMN AAVRAVVRVG IYTGARVFFV
60 70 80 90 100
HEGYQGLVDG GDNIREATWE SVSMMLQLGG TVIGSARCKD FREREGRLRA
110 120 130 140 150
AHNLVKRGIT NLCVIGGDGS LTGADTFRSE WSDLLSDLQK SGKITAEEAT
160 170 180 190 200
KSSYLNIVGL VGSIDNDFCG TDMTIGTDSA LHRIIEIVDA ITTTAQSHQR
210 220 230 240 250
TFVLEVMGRH CGYLALVTSL SCGADWVFIP ECPPDDDWEE HLCRRLSETR
260 270 280 290 300
NRGSRLNIII VAEGAIDKNG KPITSEDIKN LVVKRLGYDT RVTVLGHVQR
310 320 330 340 350
GGTPSAFDRI LGSRMGVEAV MALLEGTPDT PACVVSLSGN QAVRLPLMEC
360 370 380 390 400
VQVTKDVTRA MDERRFDEAL KLRGRSFMNN WEVYKLLAHV RPPKSKSGSH
410 420 430 440 450
TVAVMNVGAP AAGMNAAVRS TVRIGLIQGN RMLVVHDGFE GLAKGQIEEA
460 470 480 490 500
GWSYVGGWTG QGGSKLGTKR TLPKKSFEQI SANITKFNIQ GLVIIGGFEA
510 520 530 540 550
YTGGLELMEG RKQYDELCIP FVVIPATVSN NVPGSDFSVG ADTALNTICM
560 570 580 590 600
TCDRIKQSAA GTKRRVFIIE TMGGYCGYLA TMAGLAAGAD AAYIFEDPFT
610 620 630 640 650
IRDLQVNVEH LVQKMKTTVK RGLVLRNEKC NENYTTDFIF NLYSEEGKGI
660 670 680 690 700
FDSRKNVLGH MQQGGSPTPF DRNFATKMGA KAMNWMSGKI KESYRNGRIF
710 720 730 740 750
ANSPDSGCVL GMRKRALVFQ PVTELKEQTD FEHRIPKEQW WLKLRPILKI
760 770
LAKYEIDLDT SEHAHLEHIS RKRSGEANV
Length:779
Mass (Da):85,294
Last modified:October 3, 2006 - v1
Checksum:iBBA2D207ADD079EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC122655 mRNA. Translation: AAI22656.1.
RefSeqiNP_001068736.1. NM_001075268.1.
UniGeneiBt.61411.

Genome annotation databases

EnsembliENSBTAT00000000359; ENSBTAP00000000359; ENSBTAG00000000286.
GeneIDi506544.
KEGGibta:506544.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC122655 mRNA. Translation: AAI22656.1.
RefSeqiNP_001068736.1. NM_001075268.1.
UniGeneiBt.61411.

3D structure databases

ProteinModelPortaliQ0IIG5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000000358.

Proteomic databases

PaxDbiQ0IIG5.
PRIDEiQ0IIG5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000000359; ENSBTAP00000000359; ENSBTAG00000000286.
GeneIDi506544.
KEGGibta:506544.

Organism-specific databases

CTDi5213.

Phylogenomic databases

eggNOGiCOG0205.
GeneTreeiENSGT00390000013209.
HOGENOMiHOG000200154.
HOVERGENiHBG000976.
InParanoidiQ0IIG5.
KOiK00850.
OMAiVYHMASK.
OrthoDBiEOG7ZSHV5.
TreeFamiTF300411.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
ReactomeiREACT_333435. Glycolysis.

Miscellaneous databases

NextBioi20867650.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-Pfructokinase_euk.
IPR022953. ATP_PFK.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Basal ganglia.

Entry informationi

Entry nameiPFKAM_BOVIN
AccessioniPrimary (citable) accession number: Q0IIG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: October 3, 2006
Last modified: April 29, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.