6-phosphofructokinase, muscle type

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Q0IIG5 (K6PF_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 56. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
6-phosphofructokinase, muscle type
EC=2.7.1.11

Alternative name(s):
Phosphofructo-1-kinase isozyme A
Short name=PFK-A
Short name=Phosphofructokinase-M
Phosphofructokinase 1
Phosphohexokinase

Gene names

Name:

PFKM

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	779 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the third step of glycolysis, the phosphorylation of fructose-6-phosphate (F6P) by ATP to generate fructose-1,6-bisphosphate (FBP) and ADP By similarity.
Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.
Cofactor	Magnesium By similarity.
Enzyme regulation	Allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subunit structure	Homotetramer By similarity.
Post-translational modification	GlcNAcylation decreases enzyme activity By similarity.
Sequence similarities	Belongs to the phosphofructokinase family. Two domains subfamily.

Ontologies

Keywords
Biological process	Glycolysis
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation Glycoprotein Phosphoprotein
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glucose homeostasis Inferred from electronic annotation. Source: Compara glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway muscle cell homeostasis Inferred from electronic annotation. Source: Compara positive regulation of insulin secretion Inferred from electronic annotation. Source: Compara protein oligomerization Inferred from electronic annotation. Source: Compara
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro apical plasma membrane Inferred from electronic annotation. Source: Compara
Molecular_function	6-phosphofructokinase activity Inferred from sequence or structural similarity. Source: UniProtKB ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructose binding Inferred from electronic annotation. Source: Compara metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed By similarity

Chain

2 – 779

778

6-phosphofructokinase, muscle type

PRO_0000284438

Regions

Repeat

2 – 401

400

Approximate

Repeat

402 – 779

378

Approximate

Nucleotide binding

34 – 38

ATP By similarity

Nucleotide binding

192 – 196

ATP By similarity

Nucleotide binding

209 – 225

ATP By similarity

Sites

Active site

165

Proton acceptor By similarity

Metal binding

223

Magnesium; via carbonyl oxygen By similarity

Binding site

200

Substrate By similarity

Binding site

291

Substrate By similarity

Binding site

297

Substrate By similarity

Binding site

300

Substrate By similarity

Amino acid modifications

Modified residue

N-acetylthreonine By similarity

Modified residue

666

Phosphoserine By similarity

Modified residue

774

Phosphoserine By similarity

Glycosylation

529

O-linked (GlcNAc...) By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q0IIG5 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: BBA2D207ADD079EE
Show »

FASTA

779

85,294

        10         20         30         40         50         60 
MTHEHHAAKT LGIGKAIAVL TSGGDAQGMN AAVRAVVRVG IYTGARVFFV HEGYQGLVDG 

        70         80         90        100        110        120 
GDNIREATWE SVSMMLQLGG TVIGSARCKD FREREGRLRA AHNLVKRGIT NLCVIGGDGS 

       130        140        150        160        170        180 
LTGADTFRSE WSDLLSDLQK SGKITAEEAT KSSYLNIVGL VGSIDNDFCG TDMTIGTDSA 

       190        200        210        220        230        240 
LHRIIEIVDA ITTTAQSHQR TFVLEVMGRH CGYLALVTSL SCGADWVFIP ECPPDDDWEE 

       250        260        270        280        290        300 
HLCRRLSETR NRGSRLNIII VAEGAIDKNG KPITSEDIKN LVVKRLGYDT RVTVLGHVQR 

       310        320        330        340        350        360 
GGTPSAFDRI LGSRMGVEAV MALLEGTPDT PACVVSLSGN QAVRLPLMEC VQVTKDVTRA 

       370        380        390        400        410        420 
MDERRFDEAL KLRGRSFMNN WEVYKLLAHV RPPKSKSGSH TVAVMNVGAP AAGMNAAVRS 

       430        440        450        460        470        480 
TVRIGLIQGN RMLVVHDGFE GLAKGQIEEA GWSYVGGWTG QGGSKLGTKR TLPKKSFEQI 

       490        500        510        520        530        540 
SANITKFNIQ GLVIIGGFEA YTGGLELMEG RKQYDELCIP FVVIPATVSN NVPGSDFSVG 

       550        560        570        580        590        600 
ADTALNTICM TCDRIKQSAA GTKRRVFIIE TMGGYCGYLA TMAGLAAGAD AAYIFEDPFT 

       610        620        630        640        650        660 
IRDLQVNVEH LVQKMKTTVK RGLVLRNEKC NENYTTDFIF NLYSEEGKGI FDSRKNVLGH 

       670        680        690        700        710        720 
MQQGGSPTPF DRNFATKMGA KAMNWMSGKI KESYRNGRIF ANSPDSGCVL GMRKRALVFQ 

       730        740        750        760        770 
PVTELKEQTD FEHRIPKEQW WLKLRPILKI LAKYEIDLDT SEHAHLEHIS RKRSGEANV

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References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Basal ganglia.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC122655 mRNA. Translation: AAI22656.1.
IPI	IPI00704166.
RefSeq	NP_001068736.1. NM_001075268.1.
UniGene	Bt.61411.
3D structure databases
ProteinModelPortal	Q0IIG5.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000000358.
Proteomic databases
PaxDb	Q0IIG5.
PRIDE	Q0IIG5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000000359; ENSBTAP00000000359; ENSBTAG00000000286.
GeneID	506544.
KEGG	bta:506544.
Organism-specific databases
CTD	5213.
Phylogenomic databases
eggNOG	COG0205.
GeneTree	ENSGT00390000013209.
HOGENOM	HOG000200154.
HOVERGEN	HBG000976.
InParanoid	Q0IIG5.
KO	K00850.
OMA	VYHMASK.
OrthoDB	EOG4RJG0W.
Enzyme and pathway databases
UniPathway	UPA00109; UER00182.
Gene expression databases
ArrayExpress	Q0IIG5.
Family and domain databases
InterPro	IPR009161. 6-phosphofructokinase_euk. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 2 hits. [Graphical view]
PIRSF	PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 2 hits.
TIGRFAMs	TIGR02478. 6PF1K_euk. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	20867650.

Entry information

Entry name

K6PF_BOVIN

Accession

Primary (citable) accession number: Q0IIG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	October 3, 2006
Last modified:	April 3, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents