Reviewed, UniProtKB/Swiss-Prot Q0IIG5 (K6PF_BOVIN)

Names and origin

Protein names

Recommended name:
    6-phosphofructokinase, muscle type
    EC=2.7.1.11
Alternative name(s):
    Phosphofructokinase 1
    Phosphohexokinase
    Phosphofructo-1-kinase isozyme A
      Short name=PFK-A
      Short name=Phosphofructokinase-M

Gene names

Name:

PFKM

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	779 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.
Cofactor	Magnesium By similarity.
Enzyme regulation	Allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the phosphofructokinase family. Two domains subfamily.

Ontologies

Keywords
Biological process	Glycolysis
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	6-phosphofructokinase activity Inferred from sequence or structural similarity. Source: UniProtKB ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 779	778	6-phosphofructokinase, muscle type		PRO_0000284438
Regions
	Repeat	2 – 401	400	Approximate
	Repeat	402 – 779	378	Approximate
	Nucleotide binding	34 – 38	5	ATP By similarity
	Nucleotide binding	192 – 196	5	ATP By similarity
	Nucleotide binding	209 – 225	17	ATP By similarity
Sites
	Active site	165	1	Proton acceptor By similarity
	Metal binding	223	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	200	1	Substrate By similarity
	Binding site	291	1	Substrate By similarity
	Binding site	297	1	Substrate By similarity
	Binding site	300	1	Substrate By similarity
Amino acid modifications
	Modified residue	2	1	Phosphothreonine By similarity
	Modified residue	774	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0IIG5-1 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: BBA2D207ADD079EE	FASTA	779	85,294
	10 20 30 40 50 60 MTHEHHAAKT LGIGKAIAVL TSGGDAQGMN AAVRAVVRVG IYTGARVFFV HEGYQGLVDG 70 80 90 100 110 120 GDNIREATWE SVSMMLQLGG TVIGSARCKD FREREGRLRA AHNLVKRGIT NLCVIGGDGS 130 140 150 160 170 180 LTGADTFRSE WSDLLSDLQK SGKITAEEAT KSSYLNIVGL VGSIDNDFCG TDMTIGTDSA 190 200 210 220 230 240 LHRIIEIVDA ITTTAQSHQR TFVLEVMGRH CGYLALVTSL SCGADWVFIP ECPPDDDWEE 250 260 270 280 290 300 HLCRRLSETR NRGSRLNIII VAEGAIDKNG KPITSEDIKN LVVKRLGYDT RVTVLGHVQR 310 320 330 340 350 360 GGTPSAFDRI LGSRMGVEAV MALLEGTPDT PACVVSLSGN QAVRLPLMEC VQVTKDVTRA 370 380 390 400 410 420 MDERRFDEAL KLRGRSFMNN WEVYKLLAHV RPPKSKSGSH TVAVMNVGAP AAGMNAAVRS 430 440 450 460 470 480 TVRIGLIQGN RMLVVHDGFE GLAKGQIEEA GWSYVGGWTG QGGSKLGTKR TLPKKSFEQI 490 500 510 520 530 540 SANITKFNIQ GLVIIGGFEA YTGGLELMEG RKQYDELCIP FVVIPATVSN NVPGSDFSVG 550 560 570 580 590 600 ADTALNTICM TCDRIKQSAA GTKRRVFIIE TMGGYCGYLA TMAGLAAGAD AAYIFEDPFT 610 620 630 640 650 660 IRDLQVNVEH LVQKMKTTVK RGLVLRNEKC NENYTTDFIF NLYSEEGKGI FDSRKNVLGH 670 680 690 700 710 720 MQQGGSPTPF DRNFATKMGA KAMNWMSGKI KESYRNGRIF ANSPDSGCVL GMRKRALVFQ 730 740 750 760 770 PVTELKEQTD FEHRIPKEQW WLKLRPILKI LAKYEIDLDT SEHAHLEHIS RKRSGEANV

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Basal ganglia.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC122655 mRNA. Translation: AAI22656.1.
IPI	IPI00685357.
RefSeq	NP_001068736.1.
UniGene	Bt.61411
3D structure databases
SMR	Q0IIG5. Positions 13-392, 401-743.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0IIG5.
Genome annotation databases
Ensembl	ENSBTAT00000000358; ENSBTAP00000000358; ENSBTAG00000000286; Bos taurus. [Genome view] ENSBTAT00000000359; ENSBTAP00000000359; ENSBTAG00000000286; Bos taurus. [Genome view]
GeneID	506544.
KEGG	bta:506544.
Organism-specific databases
CTD	506544.
Phylogenomic databases
eggNOG	maNOG13288.
HOVERGEN	Q0IIG5.
InParanoid	Q0IIG5.
PhylomeDB	Q0IIG5.
Enzyme and pathway databases
BRENDA	2.7.1.11. 251.
Family and domain databases
InterPro	IPR009161. 6-phosphofructokinase_euk. IPR000023. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. [Graphical view]
Pfam	PF00365. PFK. 2 hits. [Graphical view]
PIRSF	PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
TIGRFAMs	TIGR02478. 6PF1K_euk. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_BOVIN

Accession

Primary (citable) accession number: Q0IIG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	October 3, 2006
Last modified:	February 9, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)