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Q0IIG5

- PFKAM_BOVIN

UniProt

Q0IIG5 - PFKAM_BOVIN

Protein

ATP-dependent 6-phosphofructokinase, muscle type

Gene

PFKM

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (03 Oct 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei24 – 241ATP; via amide nitrogenUniRule annotation
    Metal bindingi118 – 1181Magnesium; catalyticUniRule annotation
    Active sitei165 – 1651Proton acceptorUniRule annotation
    Binding sitei200 – 2001Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei263 – 2631SubstrateUniRule annotation
    Binding sitei291 – 2911Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei470 – 4701Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei565 – 5651Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
    Binding sitei628 – 6281Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei654 – 6541Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
    Binding sitei734 – 7341Allosteric activator fructose 2,6-bisphosphateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi87 – 882ATPUniRule annotation
    Nucleotide bindingi117 – 1204ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB
    2. ATP binding Source: UniProtKB-KW
    3. fructose binding Source: Ensembl
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. carbohydrate phosphorylation Source: GOC
    2. fructose 6-phosphate metabolic process Source: InterPro
    3. glucose homeostasis Source: Ensembl
    4. glycolytic process Source: UniProtKB-UniPathway
    5. muscle cell cellular homeostasis Source: Ensembl
    6. positive regulation of insulin secretion Source: Ensembl
    7. protein oligomerization Source: Ensembl

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_224187. Glycolysis.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinase, muscle typeUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PFK-M
    Alternative name(s):
    6-phosphofructokinase type A
    Phosphofructo-1-kinase isozyme A
    Short name:
    PFK-A
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:PFKM
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 5

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro
    2. apical plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 779778ATP-dependent 6-phosphofructokinase, muscle typePRO_0000284438Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylthreonineBy similarity
    Glycosylationi529 – 5291O-linked (GlcNAc)By similarity
    Modified residuei666 – 6661PhosphoserineBy similarity
    Modified residuei774 – 7741PhosphoserineBy similarity

    Post-translational modificationi

    GlcNAcylation decreases enzyme activity.By similarity

    Keywords - PTMi

    Acetylation, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ0IIG5.
    PRIDEiQ0IIG5.

    Interactioni

    Subunit structurei

    Homo- and heterotetramers.UniRule annotation

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000000358.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0IIG5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 389388N-terminal catalytic PFK domain 1Add
    BLAST
    Regioni163 – 1653Substrate bindingUniRule annotation
    Regioni207 – 2093Substrate bindingUniRule annotation
    Regioni297 – 3004Substrate bindingUniRule annotation
    Regioni390 – 40011Interdomain linkerAdd
    BLAST
    Regioni401 – 779379C-terminal regulatory PFK domain 2Add
    BLAST
    Regioni527 – 5315Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni572 – 5743Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni660 – 6634Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    GeneTreeiENSGT00390000013209.
    HOGENOMiHOG000200154.
    HOVERGENiHBG000976.
    InParanoidiQ0IIG5.
    KOiK00850.
    OMAiVYHMASK.
    OrthoDBiEOG7ZSHV5.
    TreeFamiTF300411.

    Family and domain databases

    HAMAPiMF_03184. Phosphofructokinase_I_E.
    InterProiIPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 2 hits.
    [Graphical view]
    PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 2 hits.
    TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q0IIG5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTHEHHAAKT LGIGKAIAVL TSGGDAQGMN AAVRAVVRVG IYTGARVFFV    50
    HEGYQGLVDG GDNIREATWE SVSMMLQLGG TVIGSARCKD FREREGRLRA 100
    AHNLVKRGIT NLCVIGGDGS LTGADTFRSE WSDLLSDLQK SGKITAEEAT 150
    KSSYLNIVGL VGSIDNDFCG TDMTIGTDSA LHRIIEIVDA ITTTAQSHQR 200
    TFVLEVMGRH CGYLALVTSL SCGADWVFIP ECPPDDDWEE HLCRRLSETR 250
    NRGSRLNIII VAEGAIDKNG KPITSEDIKN LVVKRLGYDT RVTVLGHVQR 300
    GGTPSAFDRI LGSRMGVEAV MALLEGTPDT PACVVSLSGN QAVRLPLMEC 350
    VQVTKDVTRA MDERRFDEAL KLRGRSFMNN WEVYKLLAHV RPPKSKSGSH 400
    TVAVMNVGAP AAGMNAAVRS TVRIGLIQGN RMLVVHDGFE GLAKGQIEEA 450
    GWSYVGGWTG QGGSKLGTKR TLPKKSFEQI SANITKFNIQ GLVIIGGFEA 500
    YTGGLELMEG RKQYDELCIP FVVIPATVSN NVPGSDFSVG ADTALNTICM 550
    TCDRIKQSAA GTKRRVFIIE TMGGYCGYLA TMAGLAAGAD AAYIFEDPFT 600
    IRDLQVNVEH LVQKMKTTVK RGLVLRNEKC NENYTTDFIF NLYSEEGKGI 650
    FDSRKNVLGH MQQGGSPTPF DRNFATKMGA KAMNWMSGKI KESYRNGRIF 700
    ANSPDSGCVL GMRKRALVFQ PVTELKEQTD FEHRIPKEQW WLKLRPILKI 750
    LAKYEIDLDT SEHAHLEHIS RKRSGEANV 779
    Length:779
    Mass (Da):85,294
    Last modified:October 3, 2006 - v1
    Checksum:iBBA2D207ADD079EE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC122655 mRNA. Translation: AAI22656.1.
    RefSeqiNP_001068736.1. NM_001075268.1.
    UniGeneiBt.61411.

    Genome annotation databases

    EnsembliENSBTAT00000000359; ENSBTAP00000000359; ENSBTAG00000000286.
    GeneIDi506544.
    KEGGibta:506544.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC122655 mRNA. Translation: AAI22656.1 .
    RefSeqi NP_001068736.1. NM_001075268.1.
    UniGenei Bt.61411.

    3D structure databases

    ProteinModelPortali Q0IIG5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000000358.

    Proteomic databases

    PaxDbi Q0IIG5.
    PRIDEi Q0IIG5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000000359 ; ENSBTAP00000000359 ; ENSBTAG00000000286 .
    GeneIDi 506544.
    KEGGi bta:506544.

    Organism-specific databases

    CTDi 5213.

    Phylogenomic databases

    eggNOGi COG0205.
    GeneTreei ENSGT00390000013209.
    HOGENOMi HOG000200154.
    HOVERGENi HBG000976.
    InParanoidi Q0IIG5.
    KOi K00850.
    OMAi VYHMASK.
    OrthoDBi EOG7ZSHV5.
    TreeFami TF300411.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    Reactomei REACT_224187. Glycolysis.

    Miscellaneous databases

    NextBioi 20867650.

    Family and domain databases

    HAMAPi MF_03184. Phosphofructokinase_I_E.
    InterProi IPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 2 hits.
    TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Basal ganglia.

    Entry informationi

    Entry nameiPFKAM_BOVIN
    AccessioniPrimary (citable) accession number: Q0IIG5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 17, 2007
    Last sequence update: October 3, 2006
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3