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Q0IIF7

- UBP14_BOVIN

UniProt

Q0IIF7 - UBP14_BOVIN

Protein

Ubiquitin carboxyl-terminal hydrolase 14

Gene

USP14

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs) By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei114 – 1141NucleophilePROSITE-ProRule annotation
    Active sitei435 – 4351Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. proteasome binding Source: UniProtKB
    3. ubiquitin thiolesterase activity Source: UniProtKB

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC19.015.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 14 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 14
    Ubiquitin thioesterase 14
    Ubiquitin-specific-processing protease 14
    Gene namesi
    Name:USP14
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    Cytoplasm By similarity. Cell membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. plasma membrane Source: UniProtKB-SubCell
    3. proteasome complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 494494Ubiquitin carboxyl-terminal hydrolase 14PRO_0000268845Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei143 – 1431PhosphoserineBy similarity
    Modified residuei235 – 2351PhosphothreonineBy similarity
    Modified residuei449 – 4491N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiQ0IIF7.

    Interactioni

    Subunit structurei

    Homodimer Potential. Interacts with FANCC, CXCR4 and ERN1 By similarity. Associates with the 26S proteasome.By similarityCurated

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000025580.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0IIF7.
    SMRiQ0IIF7. Positions 6-86, 98-483.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 8077Ubiquitin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini105 – 483379USPAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiNOG286607.
    HOGENOMiHOG000202292.
    HOVERGENiHBG054185.
    InParanoidiQ0IIF7.
    KOiK11843.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    IPR019954. Ubiquitin_CS.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    SMARTiSM00213. UBQ. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.
    PROSITEiPS00299. UBIQUITIN_1. 1 hit.
    PS50053. UBIQUITIN_2. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q0IIF7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPLYSVTVKW GKEKFEGVEL NTDEPPMVFK AQLFALTGVQ PARQKVMVKG    50
    GTLKDDDWGN IKMKNGMTVL MMGSADALPE EPSAKTVFVE DMTEEQLASA 100
    MELPCGLTNL GNTCYMNATV QCIRSVPELK DALKRYAGAL RASGEMASAQ 150
    YITAALRDLF DSMDKTSSSI PPIILLQFLH MAFPQFAEKG EQGQYLQQDA 200
    NECWVQMMRV LQQKLEAIED DTVKETDSSS ASAVTPSKKK SLIDQFFGVE 250
    FETTMKCTES EEEEVTKGKE SQLQLSCFIN QEVKYLFTGL KLRLQEEITK 300
    QSPTLQRNAL YIKSSKISRL PAYLTIQMVR FFYKEKESVN AKVLKDVKFP 350
    LMLDVYELCT PELQEKMVSF RSKFKDLEDK KVNQQPKTGD KDSSPQKEVK 400
    YEPFSFADDI GSNNCGYYDL QAVLTHQGRS SSSGHYVSWV KRKQDEWIKF 450
    DDDKVSIVTP EDILRLSGGG DWHIAYVLLY GPRRVEIMEE ESEQ 494
    Length:494
    Mass (Da):56,013
    Last modified:January 23, 2007 - v3
    Checksum:i089AE0AC7790CCC5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC122666 mRNA. Translation: AAI22667.1.
    RefSeqiNP_001068657.1. NM_001075189.1.
    UniGeneiBt.20599.

    Genome annotation databases

    GeneIDi505106.
    KEGGibta:505106.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC122666 mRNA. Translation: AAI22667.1 .
    RefSeqi NP_001068657.1. NM_001075189.1.
    UniGenei Bt.20599.

    3D structure databases

    ProteinModelPortali Q0IIF7.
    SMRi Q0IIF7. Positions 6-86, 98-483.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000025580.

    Protein family/group databases

    MEROPSi C19.015.

    Proteomic databases

    PRIDEi Q0IIF7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 505106.
    KEGGi bta:505106.

    Organism-specific databases

    CTDi 9097.

    Phylogenomic databases

    eggNOGi NOG286607.
    HOGENOMi HOG000202292.
    HOVERGENi HBG054185.
    InParanoidi Q0IIF7.
    KOi K11843.

    Miscellaneous databases

    NextBioi 20866986.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    IPR019954. Ubiquitin_CS.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    SMARTi SM00213. UBQ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    PROSITEi PS00299. UBIQUITIN_1. 1 hit.
    PS50053. UBIQUITIN_2. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal pons.
    2. "Relative structural and functional roles of multiple deubiquitylating proteins associated with mammalian 26S proteasome."
      Koulich E., Li X., DeMartino G.N.
      Mol. Biol. Cell 19:1072-1082(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: ASSOCIATION WITH THE 26S PROTEASOME.

    Entry informationi

    Entry nameiUBP14_BOVIN
    AccessioniPrimary (citable) accession number: Q0IIF7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 12, 2006
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 58 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3