Q0IIF0 (ILKAP_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Integrin-linked kinase-associated serine/threonine phosphatase 2C
Short name=ILKAP
EC=3.1.3.16

Gene names

Name:

ILKAP

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation By similarity.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Subunit structure	Interacts with ILK By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein dephosphorylation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	Integrin-linked kinase-associated serine/threonine phosphatase 2C		PRO_0000272270
Regions
	Domain	105 – 368	264	PP2C-like
Sites
	Metal binding	130	1	Manganese 1 By similarity
	Metal binding	130	1	Manganese 2 By similarity
	Metal binding	131	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	304	1	Manganese 2 By similarity
	Metal binding	359	1	Manganese 2 By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	13	1	Phosphoserine By similarity
	Modified residue	188	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0IIF0 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 3435602459B36030	FASTA	370	40,622
	10 20 30 40 50 60 MDLFGDLPEP ERSPRPAAGS GGPLLFDDLP PASSGDSGSL DTSLSEEVKN EGKGAKRKAS 70 80 90 100 110 120 DEEKNGSEEL VEKKVCKASS VIFSLKGYVA ERKGEREEMQ DAHVILNDIT AECQPPSALV 130 140 150 160 170 180 TRVSYFAVFD GHGGIRASKF AAQNLHQNLI RKFPKGDGIS VEKTVKRCLL DTFKHTDEEF 190 200 210 220 230 240 LKQASSQKPA WKDGSTATCV LAVDNTLYIA NLGDSRAILC RYNEESQKHA ALSLSKEHNP 250 260 270 280 290 300 TQYEERMRIQ KAGGNVRDGR VLGVLEVSRS IGDGQYKRCG VTSVPDIRRC QLTPNDRFIL 310 320 330 340 350 360 LACDGLFKVF TPEEAVNFIL SCLEDEKIQR REGKPTVDAR YEAACNRLAN KAVQRGSADN 370 VTVMVVRIGL

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Hippocampus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC122677 mRNA. Translation: AAI22678.1.
IPI	IPI00904514.
RefSeq	NP_001069123.1. NM_001075655.1.
UniGene	Bt.35583.
3D structure databases
ProteinModelPortal	Q0IIF0.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000013428.
Proteomic databases
PRIDE	Q0IIF0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000013428; ENSBTAP00000013428; ENSBTAG00000010176.
GeneID	514223.
KEGG	bta:514223.
Organism-specific databases
CTD	80895.
Phylogenomic databases
eggNOG	COG0631.
GeneTree	ENSGT00690000101775.
HOGENOM	HOG000233896.
HOVERGEN	HBG054286.
OMA	DIKRCQL.
Gene expression databases
ArrayExpress	Q0IIF0.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20871237.

Entry information

Entry name

ILKAP_BOVIN

Accession

Primary (citable) accession number: Q0IIF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	October 3, 2006
Last modified:	April 3, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program