Reviewed, UniProtKB/Swiss-Prot Q0IID9 (GLPK_BOVIN)

Names and origin

Protein names

Recommended name:
    Glycerol kinase
      Short name=Glycerokinase
      Short name=GK
    EC=2.7.1.30
Alternative name(s):
    ATP:glycerol 3-phosphotransferase

Gene names

Name:

GK

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism By similarity.
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate.
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
Subcellular location	Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasm By similarity. Note: Bound to the mitochondrial surface or cytoplasmic By similarity.
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Cellular component	Cytoplasm Membrane Mitochondrion Mitochondrion outer membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 559	559	Glycerol kinase		PRO_0000343676
Regions
	Nucleotide binding	433 – 437	5	ATP By similarity
Sites
	Binding site	20	1	Substrate By similarity
	Binding site	24	1	ATP By similarity
	Binding site	94	1	Substrate By similarity
	Binding site	148	1	Substrate By similarity
	Binding site	265	1	Substrate By similarity
	Binding site	287	1	ATP By similarity
	Binding site	332	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0IID9-1 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: CBD4FC2D97A0A682	FASTA	559	61,261
	10 20 30 40 50 60 MASAKKAVLG PLVGAVDQGT SSTRFLVFNS KTAELLSHHQ VEIKQEFPRE GWVEQDPKEI 70 80 90 100 110 120 LQSVYECIEK TCEKLGQLNI DISNIKAIGV SNQRETTVVW DKLTGEPLYN AVVWLDLRTQ 130 140 150 160 170 180 STVESLSKRI PGNNNFVKSK TGLPLSTYFS AVKLRWLLDN VRKVQKAVEE DRALFGTIDS 190 200 210 220 230 240 WLIWSLTGGA SGGVHCTDVT NASRTMLFNI HSLEWDKELC EFFEIPMKIL PNVRSSSEIY 250 260 270 280 290 300 GLMKISHSPK AGALEGVPIS GCLGDQSAAL VGQMCFQDGQ AKNTYGTGCF LLCNTGRKCV 310 320 330 340 350 360 FSEHGLLTTV AYKLGRDKPV YYALEGSVAI AGAVIRWLRD NLGIIKSSEE IEKLAKEVGT 370 380 390 400 410 420 SYGCYFVPAF SGLYAPYWEP SARGIICGLT QFTNKCHIAF AALEAVCFQT REILDAMNRD 430 440 450 460 470 480 CGIPLSHLQV DGGMTNNKIL MQLQADILYI PVVKPSMPET TALGAAMAAG AAEGVGVWSL 490 500 510 520 530 540 EPEDLSAVTM ERFEPQINAE ESEIRYSTWK KAVMKSMGWV TTQSSESGDP SIFCSLPLGF 550 FIVSSVVMLI GARYLSGMP

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Hippocampus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC122692 mRNA. Translation: AAI22693.1.
IPI	IPI00707036.
RefSeq	NP_001068704.1.
UniGene	Bt.18414
3D structure databases
SMR	Q0IID9. Positions 12-522.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0IID9.
Genome annotation databases
Ensembl	ENSBTAT00000030932; ENSBTAP00000030898; ENSBTAG00000008124; Bos taurus. [Genome view]
GeneID	505987.
KEGG	bta:505987.
Organism-specific databases
CTD	505987.
Phylogenomic databases
HOVERGEN	Q0IID9.
InParanoid	Q0IID9.
OMA	EESEYRY.
OrthoDB	EOG9BCH73.
PhylomeDB	Q0IID9.
Family and domain databases
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_BOVIN

Accession

Primary (citable) accession number: Q0IID9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	October 3, 2006
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)