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Protein

2-phosphoxylose phosphatase 1

Gene

pxylp1

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Responsible for the 2-O-dephosphorylation of xylose in the glycosaminoglycan-protein linkage region of proteoglycans thereby regulating the amount of mature glycosaminoglycan (GAG) chains. Sulfated glycosaminoglycans (GAGs), including heparan sulfate and chondroitin sulfate, are synthesized on the so-called common GAG-protein linkage region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser) of core proteins, which is formed by the stepwise addition of monosaccharide residues by the respective specific glycosyltransferases. Xylose 2-O-dephosphorylation during completion of linkage region formation is a prerequisite for the initiation and efficient elongation of the repeating disaccharide region of GAG chains.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei120 – 1201NucleophileBy similarity
Active sitei402 – 4021Proton donorBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: InterPro
  2. phosphatase activity Source: UniProtKB

GO - Biological processi

  1. chondroitin sulfate proteoglycan biosynthetic process Source: UniProtKB
  2. dephosphorylation Source: GOC
  3. glycosaminoglycan biosynthetic process Source: UniProtKB
  4. positive regulation of heparan sulfate proteoglycan biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
2-phosphoxylose phosphatase 1By similarity (EC:3.1.3.-By similarity)
Alternative name(s):
Acid phosphatase-like protein 2
Gene namesi
Name:pxylp1By similarity
Synonyms:acpl2
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143 Componenti: Unplaced

Organism-specific databases

XenbaseiXB-GENE-979576. pxylp1.

Subcellular locationi

  1. Golgi apparatus membrane By similarity; Single-pass type II membrane protein Sequence Analysis

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence Analysis
Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini28 – 501474LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB
  2. Golgi membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5015012-phosphoxylose phosphatase 1PRO_0000314928Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi328 – 3281N-linked (GlcNAc...)PROSITE-ProRule annotation
Glycosylationi377 – 3771N-linked (GlcNAc...)PROSITE-ProRule annotation
Glycosylationi488 – 4881N-linked (GlcNAc...)PROSITE-ProRule annotation

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ0IHQ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi8 – 136Poly-LeuSequence Analysis

Sequence similaritiesi

Belongs to the histidine acid phosphatase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG106056.
InParanoidiQ0IHQ9.

Family and domain databases

Gene3Di3.40.50.1240. 2 hits.
InterProiIPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0IHQ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLRNRFLLL LALAGLLAFL SLSLQFFSRW LPVSLQLKTE MQVFPEFPVR
60 70 80 90 100
LIQVGQGREE GLGAKNRKRI MPEPLTEPPA LNPLYEANLY CNTPGAKERS
110 120 130 140 150
MEGHAPPNLK LLSVQVIIRH GDRYPLYTIP KTKRPDIDCV LEPGRKPSHP
160 170 180 190 200
HLTDFISHMS KGVDTQMDGT LGSLPRLPNH ILCEMGELTQ TGVVQHLRNG
210 220 230 240 250
QLLKEIYLKK HRLLTSAWTA KHLYFESTGK SRTLQSGLAL LYSLLPNFDW
260 270 280 290 300
KKINVKHQWS TIFCSNHCDC PMRNHYLEEE QRRQYNFRVK NSLLEKTYIN
310 320 330 340 350
MAKIVGIPTR QLRASNPIDS LLCNFCHNAT FPCTKNGCID LEHFKVIKTH
360 370 380 390 400
QLEDEKERYE KQLYFKYALM ATHPLLNQTA NRMLRIAEGK KDELFALYSA
410 420 430 440 450
HDVTLSPILS ALGLREARFP RFAARLVFEL WHDPEKANNH YVRVLYNGED
460 470 480 490 500
VTFQTSFCRD QLRSSKRPLC PLKKFSTFVQ KDMFSSLNST SYYDACHQRL

F
Length:501
Mass (Da):57,977
Last modified:October 3, 2006 - v1
Checksum:i92FB07C4CBF11B71
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC123016 mRNA. Translation: AAI23017.1.
RefSeqiNP_001072621.1. NM_001079153.1.
UniGeneiStr.65007.

Genome annotation databases

GeneIDi780077.
KEGGixtr:780077.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC123016 mRNA. Translation: AAI23017.1.
RefSeqiNP_001072621.1. NM_001079153.1.
UniGeneiStr.65007.

3D structure databases

ProteinModelPortaliQ0IHQ9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi780077.
KEGGixtr:780077.

Organism-specific databases

CTDi92370.
XenbaseiXB-GENE-979576. pxylp1.

Phylogenomic databases

HOVERGENiHBG106056.
InParanoidiQ0IHQ9.

Family and domain databases

Gene3Di3.40.50.1240. 2 hits.
InterProiIPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiPXYP1_XENTR
AccessioniPrimary (citable) accession number: Q0IHQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: February 4, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.