Q0IH43 (OTU1_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin thioesterase OTU1
EC=3.4.19.12

Gene names

Name:

yod1

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Contains 1 C2H2-type zinc finger. Contains 1 OTU domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway Unfolded protein response
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Gene Ontology (GO)
Biological_process	ER-associated protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB cellular amino acid metabolic process Inferred from sequence or structural similarity. Source: UniProtKB endoplasmic reticulum unfolded protein response Inferred from sequence or structural similarity. Source: UniProtKB protein K48-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein K63-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	Ubiquitin thioesterase OTU1		PRO_0000282361
Regions
	Domain	105 – 230	126	OTU
	Zinc finger	274 – 298	25	C2H2-type
	Region	5 – 83	79	UBX-like
Sites
	Active site	113	1	By similarity
	Active site	116	1	Nucleophile By similarity
	Active site	298	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0IH43 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 4A0FEDC19A47E0DE	FASTA	304	33,807
	10 20 30 40 50 60 MLRLRCKTRE GTQLLQGLTD RSSIRELQER IAGVTGISGP LQRVMVGFPP LSLDLSDEEA 70 80 90 100 110 120 TLKNMSIKSG DTLIVEEDKS KLRSATPPVS KTDIGNWNAP AQPTIVRRVV PADNSCLFTS 130 140 150 160 170 180 IYYVVEGGVY DPACALEMRS LIAEIVASDQ SAYCDAVLGK SNEEYCSWIR REDTWGGAIE 190 200 210 220 230 240 VSILSKFYQC EICVVDTQTV RIDRFGEDSG YTKRVLLIYD GIHYDPLQRQ FPDPDMPPMT 250 260 270 280 290 300 VFSTTDDEAL VQAMELADDA RKKRQFTDVN QFALRCMACQ KGLTGQSAAR DHAKETGHTN FGEV

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC123321 mRNA. Translation: AAI23322.1.
RefSeq	NP_001090389.1. NM_001096920.1.
UniGene	Xl.59889.
3D structure databases
ProteinModelPortal	Q0IH43.
ModBase	Search...
Protein family/group databases
MEROPS	C85.007.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	779300.
KEGG	xla:779300.
Organism-specific databases
CTD	55432.
Xenbase	XB-GENE-998963. yod1.
Phylogenomic databases
HOVERGEN	HBG097006.
KO	K13719.
Family and domain databases
InterPro	IPR003323. OTU. IPR007087. Znf_C2H2. [Graphical view]
Pfam	PF02338. OTU. 1 hit. [Graphical view]
PROSITE	PS50802. OTU. 1 hit. PS00299. UBIQUITIN_1. False negative. PS50053. UBIQUITIN_2. False negative. PS00028. ZINC_FINGER_C2H2_1. 1 hit. PS50157. ZINC_FINGER_C2H2_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTU1_XENLA

Accession

Primary (citable) accession number: Q0IH43

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	October 3, 2006
Last modified:	April 3, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program