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Q0IH43 (OTU1_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ubiquitin thioesterase OTU1

EC=3.4.19.12
Gene names
Name:yod1
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length304 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains. Also able to hydrolyze 'Lys-11'-linked ubiquitin chains. Cleaves both polyubiquitin and di-ubiquitin By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Domain

The C2H2-type zinc finger mediates specificity for 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains but not for 'Lys-11'-linked ubiquitin chains. Selectivity for 'Lys-11'-linked ubiquitin chains is provided by recognition of the sequence surrounding 'Lys-11' in ubiquitin. The S2 site region provides specificity for longer 'Lys-11'-linked ubiquitin chains By similarity.

Sequence similarities

Contains 1 C2H2-type zinc finger.

Contains 1 OTU domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 304304Ubiquitin thioesterase OTU1
PRO_0000282361

Regions

Domain105 – 230126OTU
Zinc finger274 – 29825C2H2-type
Region5 – 8379UBX-like
Region110 – 1167Cys-loop By similarity
Region169 – 17911Variable-loop By similarity
Region219 – 2235His-loop By similarity
Region247 – 2526S2 site By similarity

Sites

Active site1131 By similarity
Active site1161Nucleophile By similarity
Active site2231 By similarity
Binding site2221Substrate; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0IH43 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: 4A0FEDC19A47E0DE

FASTA30433,807
        10         20         30         40         50         60 
MLRLRCKTRE GTQLLQGLTD RSSIRELQER IAGVTGISGP LQRVMVGFPP LSLDLSDEEA 

        70         80         90        100        110        120 
TLKNMSIKSG DTLIVEEDKS KLRSATPPVS KTDIGNWNAP AQPTIVRRVV PADNSCLFTS 

       130        140        150        160        170        180 
IYYVVEGGVY DPACALEMRS LIAEIVASDQ SAYCDAVLGK SNEEYCSWIR REDTWGGAIE 

       190        200        210        220        230        240 
VSILSKFYQC EICVVDTQTV RIDRFGEDSG YTKRVLLIYD GIHYDPLQRQ FPDPDMPPMT 

       250        260        270        280        290        300 
VFSTTDDEAL VQAMELADDA RKKRQFTDVN QFALRCMACQ KGLTGQSAAR DHAKETGHTN 


FGEV 

« Hide

References

[1]NIH - Xenopus Gene Collection (XGC) project
Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ovary.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC123321 mRNA. Translation: AAI23322.1.
RefSeqNP_001090389.1. NM_001096920.1.
UniGeneXl.59889.

3D structure databases

ProteinModelPortalQ0IH43.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC85.007.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID779300.
KEGGxla:779300.

Organism-specific databases

CTD55432.
XenbaseXB-GENE-998963. yod1.

Phylogenomic databases

HOVERGENHBG097006.
KOK13719.

Family and domain databases

InterProIPR003323. OTU.
IPR007087. Znf_C2H2.
[Graphical view]
PfamPF02338. OTU. 1 hit.
[Graphical view]
PROSITEPS50802. OTU. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameOTU1_XENLA
AccessionPrimary (citable) accession number: Q0IH43
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: October 3, 2006
Last modified: February 19, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families