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Protein

Argininosuccinate synthase

Gene

AAEL004701

Organism
Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathway: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (AAEL004701)
  3. no protein annotated in this organism
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathway: urea cycle

This protein is involved in step 1 of the subpathway that synthesizes (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline.
Proteins known to be involved in this subpathway in this organism are:
  1. Argininosuccinate synthase (AAEL004701)
This subpathway is part of the pathway urea cycle, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline, the pathway urea cycle and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361ATP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei87 – 871CitrullineBy similarity
Binding sitei92 – 921CitrullineBy similarity
Binding sitei119 – 1191AspartateBy similarity
Binding sitei123 – 1231AspartateBy similarity
Binding sitei123 – 1231CitrullineBy similarity
Binding sitei124 – 1241AspartateBy similarity
Binding sitei127 – 1271CitrullineBy similarity
Binding sitei180 – 1801CitrullineBy similarity
Binding sitei189 – 1891CitrullineBy similarity
Binding sitei270 – 2701CitrullineBy similarity
Binding sitei282 – 2821CitrullineBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPBy similarity
Nucleotide bindingi115 – 1239ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis, Urea cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
ORF Names:AAEL004701
OrganismiAedes aegypti (Yellowfever mosquito) (Culex aegypti)
Taxonomic identifieri7159 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia
ProteomesiUP000008820 Componenti: Unassembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 412412Argininosuccinate synthasePRO_0000321326Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi7159.AAEL004701-PA.

Structurei

3D structure databases

ProteinModelPortaliQ0IFL5.
SMRiQ0IFL5. Positions 4-405.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family.Curated

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
InParanoidiQ0IFL5.
KOiK01940.
OMAiLTKWQLY.
OrthoDBiEOG7PVWPB.
PhylomeDBiQ0IFL5.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0IFL5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGKEKILLA YSGGLDTSCI LKWLLEKGYE VICFMADVGQ EEDFVAAREK
60 70 80 90 100
ALRVGAKDVI IKDMKRIFVE KFVWPAIQMG LVYEDRYLLG TSLARPCISI
110 120 130 140 150
GLMESAAEHS CSIISHGATG KGNDQIRFEL SCYALDPKIK VIAPWRLPEF
160 170 180 190 200
CERFQGRKDL LDYAQKHGIP VSATPKAPWS MDANIMHISY ESGILENPAK
210 220 230 240 250
AAPEELYQMT QSVMKSSNTP IKVDITFKEG LPVTVLEHSG GKTLETPLEV
260 270 280 290 300
LTFLNKIGGE QGVGRVDLVE NRFLGLKSRG VYETPGATIL HVAHKDMEVY
310 320 330 340 350
CLDREIFRVK SFLALKMADY VYNGFWYSPE AEYVRTCLVG AQKNVSGKVT
360 370 380 390 400
LEIFKGHVIA IARESTKTIY NQELASMDVH GTLSPYAATG FIEVNAMRLK
410
EHYRVFGSAN ME
Length:412
Mass (Da):45,989
Last modified:October 3, 2006 - v1
Checksum:iBB9595CF2605F70F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH477312 Genomic DNA. Translation: EAT43888.1.
RefSeqiXP_001649600.1. XM_001649550.1.
UniGeneiAae.10323.

Genome annotation databases

EnsemblMetazoaiAAEL004701-RA; AAEL004701-PA; AAEL004701.
GeneIDi5565274.
KEGGiaag:AaeL_AAEL004701.
VectorBaseiAAEL004701. Aedes aegypti.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH477312 Genomic DNA. Translation: EAT43888.1.
RefSeqiXP_001649600.1. XM_001649550.1.
UniGeneiAae.10323.

3D structure databases

ProteinModelPortaliQ0IFL5.
SMRiQ0IFL5. Positions 4-405.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7159.AAEL004701-PA.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiAAEL004701-RA; AAEL004701-PA; AAEL004701.
GeneIDi5565274.
KEGGiaag:AaeL_AAEL004701.
VectorBaseiAAEL004701. Aedes aegypti.

Organism-specific databases

CTDi5565274.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
InParanoidiQ0IFL5.
KOiK01940.
OMAiLTKWQLY.
OrthoDBiEOG7PVWPB.
PhylomeDBiQ0IFL5.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Aedes aegypti, a major arbovirus vector."
    Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J., Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F., Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P.
    , Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L., Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J., Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E., El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I., Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H., Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S., Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P., Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J., Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W., Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O., Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R., Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B., Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T., Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.
    Science 316:1718-1723(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LVPib12.

Entry informationi

Entry nameiASSY_AEDAE
AccessioniPrimary (citable) accession number: Q0IFL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: October 3, 2006
Last modified: May 27, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.