Reviewed, UniProtKB/Swiss-Prot Q0IFL5 (ASSY_AEDAE)

Names and origin

Protein names

Recommended name:
    Argininosuccinate synthase
    EC=6.3.4.5
Alternative name(s):
    Citrulline--aspartate ligase

Gene names

ORF Names:

AAEL004701

Organism

Aedes aegypti (Yellowfever mosquito) (Culex aegypti)

Taxonomic identifier

7159 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicoidea › Culicidae › Culicinae › Culicini › Aedes › Stegomyia

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. Nitrogen metabolism; urea cycle; N(omega)-(L-arginino)succinic acid from L-aspartate and L-citrulline: step 1/1.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the argininosuccinate synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Urea cycle
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW urea cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW argininosuccinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	Argininosuccinate synthase		PRO_0000321326
Regions
	Nucleotide binding	10 – 18	9	ATP By similarity
	Nucleotide binding	115 – 123	9	ATP By similarity
Sites
	Binding site	36	1	ATP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	87	1	Citrulline By similarity
	Binding site	92	1	Citrulline By similarity
	Binding site	119	1	Aspartate By similarity
	Binding site	123	1	Aspartate By similarity
	Binding site	123	1	Citrulline By similarity
	Binding site	124	1	Aspartate By similarity
	Binding site	127	1	Citrulline By similarity
	Binding site	180	1	Citrulline By similarity
	Binding site	189	1	Citrulline By similarity
	Binding site	270	1	Citrulline By similarity
	Binding site	282	1	Citrulline By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0IFL5-1 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: BB9595CF2605F70F	FASTA	412	45,989
	10 20 30 40 50 60 MSGKEKILLA YSGGLDTSCI LKWLLEKGYE VICFMADVGQ EEDFVAAREK ALRVGAKDVI 70 80 90 100 110 120 IKDMKRIFVE KFVWPAIQMG LVYEDRYLLG TSLARPCISI GLMESAAEHS CSIISHGATG 130 140 150 160 170 180 KGNDQIRFEL SCYALDPKIK VIAPWRLPEF CERFQGRKDL LDYAQKHGIP VSATPKAPWS 190 200 210 220 230 240 MDANIMHISY ESGILENPAK AAPEELYQMT QSVMKSSNTP IKVDITFKEG LPVTVLEHSG 250 260 270 280 290 300 GKTLETPLEV LTFLNKIGGE QGVGRVDLVE NRFLGLKSRG VYETPGATIL HVAHKDMEVY 310 320 330 340 350 360 CLDREIFRVK SFLALKMADY VYNGFWYSPE AEYVRTCLVG AQKNVSGKVT LEIFKGHVIA 370 380 390 400 410 IARESTKTIY NQELASMDVH GTLSPYAATG FIEVNAMRLK EHYRVFGSAN ME

References

[1]

"Genome sequence of Aedes aegypti, a major arbovirus vector." Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J., Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F., Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P. , Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L., Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J., Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E., El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I., Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H., Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S., Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P., Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J., Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W., Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O., Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R., Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B., Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T., Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W. Science 316:1718-1723(2007) [PubMed: 17510324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Liverpool.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH477312 Genomic DNA. Translation: EAT43888.1.
RefSeq	XP_001649600.1.
UniGene	Aae.10323
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5565274.
KEGG	aag:AaeL_AAEL004701.
VectorBase	AAEL004701. Aedes aegypti.
Phylogenomic databases
OMA	Q0IFL5. IAPVREW.
Enzyme and pathway databases
BRENDA	6.3.4.5. 1026.
Family and domain databases
InterPro	IPR001518. Arginosuc_synth. IPR018223. Arginosuc_synth_CS. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11587. Arginosuc_synth. 1 hit.
Pfam	PF00764. Arginosuc_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00032. argG. 1 hit.
PROSITE	PS00564. ARGININOSUCCIN_SYN_1. 1 hit. PS00565. ARGININOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASSY_AEDAE

Accession

Primary (citable) accession number: Q0IFL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	October 3, 2006
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)