Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q0IEN3 (TRMB_AEDAE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
ORF Names:AAEL009156
OrganismAedes aegypti (Yellowfever mosquito) (Culex aegypti) [Reference proteome]
Taxonomic identifier7159 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia

Protein attributes

Sequence length245 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 245245tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370564

Regions

Region93 – 942S-adenosyl-L-methionine binding By similarity
Region126 – 1272S-adenosyl-L-methionine binding By similarity
Region224 – 2263S-adenosyl-L-methionine binding By similarity

Sites

Active site1491 By similarity
Binding site701S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1461S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0IEN3 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: E02F7B6314752947

FASTA24528,519
        10         20         30         40         50         60 
MEVEESKNDA SVVASPAPTK LPQKRFYRQR AHSNPIADHS FDYPIHPDDY DWSQHYPTIG 

        70         80         90        100        110        120 
DKRVEFADIG CGYGGFLVTL GEMFPDKFAV GMEIRVKVSD YVMDRIQALR KLNEGQYENI 

       130        140        150        160        170        180 
ACIRTNAMKY LTNFFHKGQL EKMFFLYPDP HFKKAKHKWR IINSALLSEY SYVLRQGGLI 

       190        200        210        220        230        240 
YTITDVKDLH EWMCKHIEQH PAFERLTDDE VKADVLSEKL LDSSEEGKKV TRNKGDKFVA 


IFRKI 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CH477558 Genomic DNA. Translation: EAT39016.1.
RefSeqXP_001653710.1. XM_001653660.1.
UniGeneAae.5992.

3D structure databases

ProteinModelPortalQ0IEN3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7159.AAEL009156-PA.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaAAEL009156-RA; AAEL009156-PA; AAEL009156.
GeneID5571573.
KEGGaag:AaeL_AAEL009156.
VectorBaseAAEL009156. Aedes aegypti.

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000260965.
KOK03439.
OMARAHSNPI.
OrthoDBEOG7PS1GC.
PhylomeDBQ0IEN3.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_AEDAE
AccessionPrimary (citable) accession number: Q0IEN3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 3, 2006
Last modified: June 11, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways