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Q0IEN3

- TRMB_AEDAE

UniProt

Q0IEN3 - TRMB_AEDAE

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

AAEL009156

Organism
Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (03 Oct 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei70 – 701S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei146 – 1461S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Active sitei149 – 1491UniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
    2. tRNA binding Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine, tRNA-binding

    Enzyme and pathway databases

    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    ORF Names:AAEL009156
    OrganismiAedes aegypti (Yellowfever mosquito) (Culex aegypti)
    Taxonomic identifieri7159 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia
    ProteomesiUP000008820: Unassembled WGS sequence

    Subcellular locationi

    Nucleus UniRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 245245tRNA (guanine-N(7)-)-methyltransferasePRO_0000370564Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi7159.AAEL009156-PA.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0IEN3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni93 – 942S-adenosyl-L-methionine bindingUniRule annotation
    Regioni126 – 1272S-adenosyl-L-methionine bindingUniRule annotation
    Regioni224 – 2263S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000260965.
    KOiK03439.
    OMAiRAHSNPI.
    OrthoDBiEOG7PS1GC.
    PhylomeDBiQ0IEN3.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q0IEN3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEVEESKNDA SVVASPAPTK LPQKRFYRQR AHSNPIADHS FDYPIHPDDY    50
    DWSQHYPTIG DKRVEFADIG CGYGGFLVTL GEMFPDKFAV GMEIRVKVSD 100
    YVMDRIQALR KLNEGQYENI ACIRTNAMKY LTNFFHKGQL EKMFFLYPDP 150
    HFKKAKHKWR IINSALLSEY SYVLRQGGLI YTITDVKDLH EWMCKHIEQH 200
    PAFERLTDDE VKADVLSEKL LDSSEEGKKV TRNKGDKFVA IFRKI 245
    Length:245
    Mass (Da):28,519
    Last modified:October 3, 2006 - v1
    Checksum:iE02F7B6314752947
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH477558 Genomic DNA. Translation: EAT39016.1.
    RefSeqiXP_001653710.1. XM_001653660.1.
    UniGeneiAae.5992.

    Genome annotation databases

    EnsemblMetazoaiAAEL009156-RA; AAEL009156-PA; AAEL009156.
    GeneIDi5571573.
    KEGGiaag:AaeL_AAEL009156.
    VectorBaseiAAEL009156. Aedes aegypti.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH477558 Genomic DNA. Translation: EAT39016.1 .
    RefSeqi XP_001653710.1. XM_001653660.1.
    UniGenei Aae.5992.

    3D structure databases

    ProteinModelPortali Q0IEN3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7159.AAEL009156-PA.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai AAEL009156-RA ; AAEL009156-PA ; AAEL009156 .
    GeneIDi 5571573.
    KEGGi aag:AaeL_AAEL009156.
    VectorBasei AAEL009156. Aedes aegypti.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000260965.
    KOi K03439.
    OMAi RAHSNPI.
    OrthoDBi EOG7PS1GC.
    PhylomeDBi Q0IEN3.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Aedes aegypti, a major arbovirus vector."
      Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J., Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F., Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P.
      , Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L., Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J., Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E., El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I., Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H., Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S., Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P., Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J., Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W., Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O., Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R., Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B., Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T., Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.
      Science 316:1718-1723(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LVPib12.

    Entry informationi

    Entry nameiTRMB_AEDAE
    AccessioniPrimary (citable) accession number: Q0IEN3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: October 3, 2006
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3