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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

AAEL009156

Organism
Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei70S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei146S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei149UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
ORF Names:AAEL009156
OrganismiAedes aegypti (Yellowfever mosquito) (Culex aegypti)
Taxonomic identifieri7159 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia
Proteomesi
  • UP000008820 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003705641 – 245tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST245

Interactioni

Protein-protein interaction databases

STRINGi7159.AAEL009156-PA.

Structurei

3D structure databases

ProteinModelPortaliQ0IEN3.
SMRiQ0IEN3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni93 – 94S-adenosyl-L-methionine bindingUniRule annotation2
Regioni126 – 127S-adenosyl-L-methionine bindingUniRule annotation2
Regioni224 – 226S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3115. Eukaryota.
COG0220. LUCA.
HOGENOMiHOG000260965.
InParanoidiQ0IEN3.
KOiK03439.
OMAiIGMEIRV.
OrthoDBiEOG091G0IQ7.
PhylomeDBiQ0IEN3.

Family and domain databases

HAMAPiMF_03055. tRNA_methyltr_TrmB_euk. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PANTHERiPTHR23417. PTHR23417. 1 hit.
PTHR23417:SF16. PTHR23417:SF16. 1 hit.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0IEN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVEESKNDA SVVASPAPTK LPQKRFYRQR AHSNPIADHS FDYPIHPDDY
60 70 80 90 100
DWSQHYPTIG DKRVEFADIG CGYGGFLVTL GEMFPDKFAV GMEIRVKVSD
110 120 130 140 150
YVMDRIQALR KLNEGQYENI ACIRTNAMKY LTNFFHKGQL EKMFFLYPDP
160 170 180 190 200
HFKKAKHKWR IINSALLSEY SYVLRQGGLI YTITDVKDLH EWMCKHIEQH
210 220 230 240
PAFERLTDDE VKADVLSEKL LDSSEEGKKV TRNKGDKFVA IFRKI
Length:245
Mass (Da):28,519
Last modified:October 3, 2006 - v1
Checksum:iE02F7B6314752947
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH477558 Genomic DNA. Translation: EAT39016.1.
RefSeqiXP_001653710.1. XM_001653660.1.
UniGeneiAae.5992.

Genome annotation databases

EnsemblMetazoaiAAEL009156-RA; AAEL009156-PA; AAEL009156.
GeneIDi5571573.
KEGGiaag:AaeL_AAEL009156.
VectorBaseiAAEL009156-RA; AAEL009156-PA; AAEL009156.

Similar proteinsi

Entry informationi

Entry nameiTRMB_AEDAE
AccessioniPrimary (citable) accession number: Q0IEN3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 3, 2006
Last modified: October 25, 2017
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families