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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Synechococcus sp. (strain CC9311)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351S-adenosyl-L-methionineUniRule annotation
Binding sitei60 – 601S-adenosyl-L-methionineUniRule annotation
Binding sitei87 – 871S-adenosyl-L-methionineUniRule annotation
Active sitei113 – 1131By similarity
Binding sitei113 – 1131S-adenosyl-L-methionineUniRule annotation
Binding sitei117 – 1171SubstrateUniRule annotation
Binding sitei149 – 1491SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSSP64471:GIVP-328-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:sync_0328
OrganismiSynechococcus sp. (strain CC9311)
Taxonomic identifieri64471 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
ProteomesiUP000001961 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 237237tRNA (guanine-N(7)-)-methyltransferasePRO_0000288242Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi64471.sync_0328.

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000237936.
KOiK03439.
OMAiVNPLSRF.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0IDB0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQHVNPLSR FFQLPLELPA PHELFEHPNL PIHLDIGCAR GFFLLELAAM
60 70 80 90 100
QPERNHLGVE IRRPLVQAAQ HDRDRQEQHN LHFLFCNANI SLEAWMAALP
110 120 130 140 150
PDQLQLVSIQ FPDPWFKQRH RKRRVLQPAL LLAIAAALHP GRHLFLQSDV
160 170 180 190 200
LAVIEPMVAL VELSNCFARP KDDPQPWRSS NPLPVATERE RYVQEQGQPT
210 220 230
YRVLFERTQA ELPALRDLEM AWQQVDNSKD AAPTPHG
Length:237
Mass (Da):27,319
Last modified:October 3, 2006 - v1
Checksum:iE6D88F5D695FBEAE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000435 Genomic DNA. Translation: ABI47587.1.
RefSeqiWP_011618304.1. NC_008319.1.
YP_729562.1. NC_008319.1.

Genome annotation databases

EnsemblBacteriaiABI47587; ABI47587; sync_0328.
KEGGisyg:sync_0328.
PATRICi23791512. VBISynSp88089_0338.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000435 Genomic DNA. Translation: ABI47587.1.
RefSeqiWP_011618304.1. NC_008319.1.
YP_729562.1. NC_008319.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi64471.sync_0328.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI47587; ABI47587; sync_0328.
KEGGisyg:sync_0328.
PATRICi23791512. VBISynSp88089_0338.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000237936.
KOiK03439.
OMAiVNPLSRF.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciSSP64471:GIVP-328-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CC9311.

Entry informationi

Entry nameiTRMB_SYNS3
AccessioniPrimary (citable) accession number: Q0IDB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 3, 2006
Last modified: April 1, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.