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Q0IDA5

- SYI_SYNS3

UniProt

Q0IDA5 - SYI_SYNS3

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Synechococcus sp. (strain CC9311)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (03 Oct 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei584 – 5841Aminoacyl-adenylateUniRule annotation
    Binding sitei628 – 6281ATPUniRule annotation
    Metal bindingi938 – 9381ZincUniRule annotation
    Metal bindingi941 – 9411ZincUniRule annotation
    Metal bindingi958 – 9581ZincUniRule annotation
    Metal bindingi961 – 9611ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciSSP64471:GIVP-333-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:sync_0333
    OrganismiSynechococcus sp. (strain CC9311)
    Taxonomic identifieri64471 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
    ProteomesiUP000001961: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 968968Isoleucine--tRNA ligasePRO_1000022135Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi64471.sync_0333.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0IDA5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi68 – 7811"HIGH" regionAdd
    BLAST
    Motifi625 – 6295"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q0IDA5-1 [UniParc]FASTAAdd to Basket

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    MTQQTGQDAD QRPSYKDTLN LLETGFGMRA NAIHREPELQ AFWKEKGIDL    50
    DLGRNNPGPV FTLHDGPPYA NGALHMGHAL NKVLKDIINK HRLMQGRKVR 100
    FVPGWDCHGL PIELKVLQAM NQEQRQALTP IKLRKKAAAY AHKQVAGQRA 150
    GFQRWGIWAD WDHPYLTLQK DYEAAQIDVF GTMALKGHIY RGLKPVHWSP 200
    SSRTALAEAE LEYPDGHTSP SVYVGFPVVD LPESLRSKLN AQGLDVPAAS 250
    DTLSQCLQVA IWTTTPWTLP ANLAVSVNDR LDYCLADDGN GQLLIVAAEL 300
    CDSIASKLER PLQAKATVKG ADLAGITYSH PLLERRSAIV VGGEYITTES 350
    GTGLVHTAPG HGVDDFNTGR KHGLPVLCPV DEAGTLTAEA GPFEGLNVLK 400
    DANAKIIAAL EDSGSLLLQE SYSHRYPYDW RTKKPTIFRA TEQWFASVEG 450
    FRTEALTAID GVQWLPASGR NRIESMVSER GDWCISRQRT WGVPIPVFYQ 500
    RETGEVLLNS DSIAHVKALI AEHGADIWWE KDEVDLLPSS HKAEAHLWRK 550
    GTDTMDVWFD SGSSWASVSS QRDGLSYPAD LYLEGSDQHR GWFQSSLLTS 600
    VAVNGTAPYR TVLTHGFALD EKGRKMSKSL GNVVDPMVII EGGKNQKQEP 650
    AYGADVLRLW VSSVDYSADV PIGAGILRQL SDVYRKVRNT SRYLLGNLHD 700
    FIPSRDAISI SDLPLLDRWM LQRTATVLDQ ISEAFERYEF FRFFQLLQNF 750
    CVADLSNFYL DIAKDRLYVS APNDKRRRSC QTVMALIIER LAAAIAPVLC 800
    HMAEDIWQNI PYPTGTESVF LSGWPSVPEE WRDDSLRDPM QELRELRAAV 850
    NKVLEECRSK RKLGSSLEAA VRLEARTPAL QDALQWLQSK GDQEVDGLRD 900
    WLLVSQLQIG GEPWAELLAS DDNELAVIEV ALSRGQKCER CWHYEADIGQ 950
    YSDHPGLCGR CVSVLERR 968
    Length:968
    Mass (Da):108,080
    Last modified:October 3, 2006 - v1
    Checksum:iB761E5BA2DE8B901
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000435 Genomic DNA. Translation: ABI45549.1.
    RefSeqiWP_011618309.1. NC_008319.1.
    YP_729567.1. NC_008319.1.

    Genome annotation databases

    EnsemblBacteriaiABI45549; ABI45549; sync_0333.
    GeneIDi4260647.
    KEGGisyg:sync_0333.
    PATRICi23791520. VBISynSp88089_0342.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000435 Genomic DNA. Translation: ABI45549.1 .
    RefSeqi WP_011618309.1. NC_008319.1.
    YP_729567.1. NC_008319.1.

    3D structure databases

    ProteinModelPortali Q0IDA5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 64471.sync_0333.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABI45549 ; ABI45549 ; sync_0333 .
    GeneIDi 4260647.
    KEGGi syg:sync_0333.
    PATRICi 23791520. VBISynSp88089_0342.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci SSP64471:GIVP-333-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CC9311.

    Entry informationi

    Entry nameiSYI_SYNS3
    AccessioniPrimary (citable) accession number: Q0IDA5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: October 3, 2006
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3