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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Synechococcus sp. (strain CC9311)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei584 – 5841Aminoacyl-adenylateUniRule annotation
Binding sitei628 – 6281ATPUniRule annotation
Metal bindingi938 – 9381ZincUniRule annotation
Metal bindingi941 – 9411ZincUniRule annotation
Metal bindingi958 – 9581ZincUniRule annotation
Metal bindingi961 – 9611ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSSP64471:GIVP-333-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:sync_0333
OrganismiSynechococcus sp. (strain CC9311)
Taxonomic identifieri64471 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
ProteomesiUP000001961 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 968968Isoleucine--tRNA ligasePRO_1000022135Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi64471.sync_0333.

Structurei

3D structure databases

ProteinModelPortaliQ0IDA5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi68 – 7811"HIGH" regionAdd
BLAST
Motifi625 – 6295"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0IDA5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQQTGQDAD QRPSYKDTLN LLETGFGMRA NAIHREPELQ AFWKEKGIDL
60 70 80 90 100
DLGRNNPGPV FTLHDGPPYA NGALHMGHAL NKVLKDIINK HRLMQGRKVR
110 120 130 140 150
FVPGWDCHGL PIELKVLQAM NQEQRQALTP IKLRKKAAAY AHKQVAGQRA
160 170 180 190 200
GFQRWGIWAD WDHPYLTLQK DYEAAQIDVF GTMALKGHIY RGLKPVHWSP
210 220 230 240 250
SSRTALAEAE LEYPDGHTSP SVYVGFPVVD LPESLRSKLN AQGLDVPAAS
260 270 280 290 300
DTLSQCLQVA IWTTTPWTLP ANLAVSVNDR LDYCLADDGN GQLLIVAAEL
310 320 330 340 350
CDSIASKLER PLQAKATVKG ADLAGITYSH PLLERRSAIV VGGEYITTES
360 370 380 390 400
GTGLVHTAPG HGVDDFNTGR KHGLPVLCPV DEAGTLTAEA GPFEGLNVLK
410 420 430 440 450
DANAKIIAAL EDSGSLLLQE SYSHRYPYDW RTKKPTIFRA TEQWFASVEG
460 470 480 490 500
FRTEALTAID GVQWLPASGR NRIESMVSER GDWCISRQRT WGVPIPVFYQ
510 520 530 540 550
RETGEVLLNS DSIAHVKALI AEHGADIWWE KDEVDLLPSS HKAEAHLWRK
560 570 580 590 600
GTDTMDVWFD SGSSWASVSS QRDGLSYPAD LYLEGSDQHR GWFQSSLLTS
610 620 630 640 650
VAVNGTAPYR TVLTHGFALD EKGRKMSKSL GNVVDPMVII EGGKNQKQEP
660 670 680 690 700
AYGADVLRLW VSSVDYSADV PIGAGILRQL SDVYRKVRNT SRYLLGNLHD
710 720 730 740 750
FIPSRDAISI SDLPLLDRWM LQRTATVLDQ ISEAFERYEF FRFFQLLQNF
760 770 780 790 800
CVADLSNFYL DIAKDRLYVS APNDKRRRSC QTVMALIIER LAAAIAPVLC
810 820 830 840 850
HMAEDIWQNI PYPTGTESVF LSGWPSVPEE WRDDSLRDPM QELRELRAAV
860 870 880 890 900
NKVLEECRSK RKLGSSLEAA VRLEARTPAL QDALQWLQSK GDQEVDGLRD
910 920 930 940 950
WLLVSQLQIG GEPWAELLAS DDNELAVIEV ALSRGQKCER CWHYEADIGQ
960
YSDHPGLCGR CVSVLERR
Length:968
Mass (Da):108,080
Last modified:October 3, 2006 - v1
Checksum:iB761E5BA2DE8B901
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000435 Genomic DNA. Translation: ABI45549.1.
RefSeqiWP_011618309.1. NC_008319.1.
YP_729567.1. NC_008319.1.

Genome annotation databases

EnsemblBacteriaiABI45549; ABI45549; sync_0333.
KEGGisyg:sync_0333.
PATRICi23791520. VBISynSp88089_0342.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000435 Genomic DNA. Translation: ABI45549.1.
RefSeqiWP_011618309.1. NC_008319.1.
YP_729567.1. NC_008319.1.

3D structure databases

ProteinModelPortaliQ0IDA5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi64471.sync_0333.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI45549; ABI45549; sync_0333.
KEGGisyg:sync_0333.
PATRICi23791520. VBISynSp88089_0342.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciSSP64471:GIVP-333-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CC9311.

Entry informationi

Entry nameiSYI_SYNS3
AccessioniPrimary (citable) accession number: Q0IDA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: April 1, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.