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Q0IDA5

- SYI_SYNS3

UniProt

Q0IDA5 - SYI_SYNS3

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Protein
Isoleucine--tRNA ligase
Gene
ileS, sync_0333
Organism
Synechococcus sp. (strain CC9311)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei584 – 5841Aminoacyl-adenylate By similarity
Binding sitei628 – 6281ATP By similarity
Metal bindingi938 – 9381Zinc By similarity
Metal bindingi941 – 9411Zinc By similarity
Metal bindingi958 – 9581Zinc By similarity
Metal bindingi961 – 9611Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSSP64471:GIVP-333-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:sync_0333
OrganismiSynechococcus sp. (strain CC9311)
Taxonomic identifieri64471 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
ProteomesiUP000001961: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 968968Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022135Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi64471.sync_0333.

Structurei

3D structure databases

ProteinModelPortaliQ0IDA5.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi68 – 7811"HIGH" regionUniRule annotation
Add
BLAST
Motifi625 – 6295"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0IDA5-1 [UniParc]FASTAAdd to Basket

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MTQQTGQDAD QRPSYKDTLN LLETGFGMRA NAIHREPELQ AFWKEKGIDL    50
DLGRNNPGPV FTLHDGPPYA NGALHMGHAL NKVLKDIINK HRLMQGRKVR 100
FVPGWDCHGL PIELKVLQAM NQEQRQALTP IKLRKKAAAY AHKQVAGQRA 150
GFQRWGIWAD WDHPYLTLQK DYEAAQIDVF GTMALKGHIY RGLKPVHWSP 200
SSRTALAEAE LEYPDGHTSP SVYVGFPVVD LPESLRSKLN AQGLDVPAAS 250
DTLSQCLQVA IWTTTPWTLP ANLAVSVNDR LDYCLADDGN GQLLIVAAEL 300
CDSIASKLER PLQAKATVKG ADLAGITYSH PLLERRSAIV VGGEYITTES 350
GTGLVHTAPG HGVDDFNTGR KHGLPVLCPV DEAGTLTAEA GPFEGLNVLK 400
DANAKIIAAL EDSGSLLLQE SYSHRYPYDW RTKKPTIFRA TEQWFASVEG 450
FRTEALTAID GVQWLPASGR NRIESMVSER GDWCISRQRT WGVPIPVFYQ 500
RETGEVLLNS DSIAHVKALI AEHGADIWWE KDEVDLLPSS HKAEAHLWRK 550
GTDTMDVWFD SGSSWASVSS QRDGLSYPAD LYLEGSDQHR GWFQSSLLTS 600
VAVNGTAPYR TVLTHGFALD EKGRKMSKSL GNVVDPMVII EGGKNQKQEP 650
AYGADVLRLW VSSVDYSADV PIGAGILRQL SDVYRKVRNT SRYLLGNLHD 700
FIPSRDAISI SDLPLLDRWM LQRTATVLDQ ISEAFERYEF FRFFQLLQNF 750
CVADLSNFYL DIAKDRLYVS APNDKRRRSC QTVMALIIER LAAAIAPVLC 800
HMAEDIWQNI PYPTGTESVF LSGWPSVPEE WRDDSLRDPM QELRELRAAV 850
NKVLEECRSK RKLGSSLEAA VRLEARTPAL QDALQWLQSK GDQEVDGLRD 900
WLLVSQLQIG GEPWAELLAS DDNELAVIEV ALSRGQKCER CWHYEADIGQ 950
YSDHPGLCGR CVSVLERR 968
Length:968
Mass (Da):108,080
Last modified:October 3, 2006 - v1
Checksum:iB761E5BA2DE8B901
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000435 Genomic DNA. Translation: ABI45549.1.
RefSeqiWP_011618309.1. NC_008319.1.
YP_729567.1. NC_008319.1.

Genome annotation databases

EnsemblBacteriaiABI45549; ABI45549; sync_0333.
GeneIDi4260647.
KEGGisyg:sync_0333.
PATRICi23791520. VBISynSp88089_0342.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000435 Genomic DNA. Translation: ABI45549.1 .
RefSeqi WP_011618309.1. NC_008319.1.
YP_729567.1. NC_008319.1.

3D structure databases

ProteinModelPortali Q0IDA5.
ModBasei Search...

Protein-protein interaction databases

STRINGi 64471.sync_0333.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABI45549 ; ABI45549 ; sync_0333 .
GeneIDi 4260647.
KEGGi syg:sync_0333.
PATRICi 23791520. VBISynSp88089_0342.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SSP64471:GIVP-333-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CC9311.

Entry informationi

Entry nameiSYI_SYNS3
AccessioniPrimary (citable) accession number: Q0IDA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: September 3, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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