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Q0IBC8

- BIOB_SYNS3

UniProt

Q0IBC8 - BIOB_SYNS3

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Protein

Biotin synthase

Gene

bioB

Organism
Synechococcus sp. (strain CC9311)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi57 – 571Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi61 – 611Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi64 – 641Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi101 – 1011Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi133 – 1331Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi193 – 1931Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi265 – 2651Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSSP64471:GIVP-1032-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:sync_1032
OrganismiSynechococcus sp. (strain CC9311)
Taxonomic identifieri64471 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
ProteomesiUP000001961: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328Biotin synthasePRO_0000381680Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi64471.sync_1032.

Structurei

3D structure databases

ProteinModelPortaliQ0IBC8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0IBC8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTEAVEVRHD WTRSEIEALL DLPLMDLLWR AQGVHRASNP GYHVQLASLL
60 70 80 90 100
SVKTGGCEED CAYCPQSMHH SSDVTGQPEL QVAPVLERAK AAKQAGADRF
110 120 130 140 150
CMGWAWREIR DGAPFEAMLQ MVSGVRALGM EACVTAGMLT DGQAQRLAKA
160 170 180 190 200
GLTAYNHNLD TSPEHYDKII TTRTFQERLE TLERVRQAGV TLCCGGIIGM
210 220 230 240 250
GETIGDRASM LQVLASINPH PESVPINALV AVEGTPLEEL PPIDPIELVR
260 270 280 290 300
MIAVTRILMP GSRVRLSAGR EQLSKEAQIL CLQAGADSIF YGETLLTTGN
310 320
PAVEADRELL RTAGVQANWL SASEKLAA
Length:328
Mass (Da):35,594
Last modified:July 28, 2009 - v2
Checksum:iCC9BBA7C80CD4E97
GO

Sequence cautioni

The sequence ABI46705.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000435 Genomic DNA. Translation: ABI46705.1. Different initiation.
RefSeqiWP_011618966.1. NC_008319.1.
YP_730244.1. NC_008319.1.

Genome annotation databases

EnsemblBacteriaiABI46705; ABI46705; sync_1032.
GeneIDi4259028.
KEGGisyg:sync_1032.
PATRICi23793042. VBISynSp88089_1084.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000435 Genomic DNA. Translation: ABI46705.1 . Different initiation.
RefSeqi WP_011618966.1. NC_008319.1.
YP_730244.1. NC_008319.1.

3D structure databases

ProteinModelPortali Q0IBC8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 64471.sync_1032.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABI46705 ; ABI46705 ; sync_1032 .
GeneIDi 4259028.
KEGGi syg:sync_1032.
PATRICi 23793042. VBISynSp88089_1084.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci SSP64471:GIVP-1032-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CC9311.

Entry informationi

Entry nameiBIOB_SYNS3
AccessioniPrimary (citable) accession number: Q0IBC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: November 26, 2014
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3