Q0I9S4 (ISPE_SYNS3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	sync_1593

Organism

Synechococcus sp. (strain CC9311) [Complete proteome] [HAMAP]

Taxonomic identifier

64471 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000335762
Regions
	Nucleotide binding	97 – 107	11	ATP Potential
Sites
	Active site	12	1	By similarity
	Active site	139	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0I9S4 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: AFA9D96561787E83	FASTA	310	33,122
	10 20 30 40 50 60 MTATVRVTAP AKINLHLEVL GQRSDGFHEL AMVMQSIDLA DQLDCSNRAD GLIQLSCDQP 70 80 90 100 110 120 GLSCGNDNLV FRAAALLRQR SGFHELGAQI HLRKRIPIGA GLAGGSSDGA AALLALNTLW 130 140 150 160 170 180 GLGHTHDHLC AMAAELGSDM PFCLAGGIQL CFGRGECLES VPAAEQSLGV VLVKNPTVSV 190 200 210 220 230 240 STPWAYGECR RLKGDHYLSD EVAFAQRRQD LRAASWLNPL RAAEPPPLRN DLQDVVEPQT 250 260 270 280 290 300 ASVQMALRLL QDLPGQLRTA MSGSGPSCFA LFPNRLDADQ ALDVARDSFA QAGFDAWSCS 310 FVGHGAKLMP

References

[1]

"Genome sequence of Synechococcus CC9311: insights into adaptation to a coastal environment." Palenik B., Ren Q., Dupont C.L., Myers G.S., Heidelberg J.F., Badger J.H., Madupu R., Nelson W.C., Brinkac L.M., Dodson R.J., Durkin A.S., Daugherty S.C., Sullivan S.A., Khouri H., Mohamoud Y., Halpin R., Paulsen I.T. Proc. Natl. Acad. Sci. U.S.A. 103:13555-13559(2006) [PubMed: 16938853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CC9311.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000435 Genomic DNA. Translation: ABI47759.1.
RefSeq	YP_730798.1. NC_008319.1.
3D structure databases
ProteinModelPortal	Q0I9S4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0I9S4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4259536.
GenomeReviews	Gene locus sync_1593 in contig CP000435_GR.
KEGG	syg:sync_1593.
PATRIC	23794218. VBISynSp88089_1666.
TIGR	sync_1593.
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	QLCFGRG.
PhylomeDB	Q0I9S4.
ProtClustDB	PRK02534.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_SYNS3

Accession

Primary (citable) accession number: Q0I9S4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 3, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program