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Q0I5G9

- NAPA_HISS1

UniProt

Q0I5G9 - NAPA_HISS1

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Histophilus somni (strain 129Pt) (Haemophilus somnus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (03 Oct 2006)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi44 – 441Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi79 – 791Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciHSOM205914:GJ7V-1575-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:HS_1508
    OrganismiHistophilus somni (strain 129Pt) (Haemophilus somnus)
    Taxonomic identifieri205914 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHistophilus
    ProteomesiUP000001970: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232Tat-type signalUniRule annotationAdd
    BLAST
    Chaini33 – 827795Periplasmic nitrate reductasePRO_1000069720Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi205914.HS_1508.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0I5G9.
    SMRiQ0I5G9. Positions 36-826.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini37 – 93574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q0I5G9-1 [UniParc]FASTAAdd to Basket

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    MNLSRRDFMK ANAALAAASV AGLIIPVKNV NAADTSITWD KAVCRFCGTG    50
    CAVLVGTKDG RVVASQGDPD AEVNRGLNCI KGYFLPKIMY GKDRLTHPML 100
    RMKNGQYDKE GEFTPVTWDF AFKTMAEKFK SALKAKGPNG VGMFTSGQST 150
    IFEGVAKSKL FKAGLLSNNI DPNARHCMAS AAVAFVRTFG IDEPMGCYDD 200
    IEHADAFVLW GSNMAEMHPI LWSRISDRRL ANPDTVSVNV LSTFEHRSFE 250
    LADLGILLKP QSDLAILNYI ANYLIENNAI NREFIEKHTK FKRGETDIGY 300
    GLRPQDPREQ IAKNVKTAGK MYDSSFEEFK KLVAPYTLEK AHEISGVPKE 350
    QLEKLAKLYA DPNKKVVSYW TMGINQHTRG VWANHLIYNI HLLTGKISLP 400
    GCGPFSLTGQ PSACGTAREV GTFIHRLPAD LVVTKPEHRK IAEKIWKLPE 450
    GLISDKLGFH AVAQSRALKD GKMQVLWQMC NNNMQAGPNI NEETYPGWRN 500
    PDNFIVVSDP YPTVSALSAD LILPTAMWVE KEGAYGNAER RTQFWRQQVK 550
    APGEAKSDLW QLVEFSKYFT TDEVWPAEIL AKNPAYQGKT LYEVLYLNGQ 600
    VNQYSNDELK GRLNDEAYHF GFYIQKGLFE EYASFGRGHG HDLADFDTYH 650
    KARGLRWPVV DGKETLWRYR EGYDPYVKAG EGVSFYGQAD KRAVILAVPY 700
    EPPAEVPDRE YDLWLTTGRI LEHWHTGSMT RRVPELHRSF PNNLVWMNPN 750
    DAKKRGLKHG DKIKVISRRG EITSYIDTRG RNKCPEGLIY TTFFDAGQLA 800
    NKLILDATDP ISKETDFKKC AVKVVKA 827
    Length:827
    Mass (Da):93,016
    Last modified:October 3, 2006 - v1
    Checksum:i19DCB138A81CCC7E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000436 Genomic DNA. Translation: ABI25781.1.
    RefSeqiYP_719718.1. NC_008309.1.

    Genome annotation databases

    EnsemblBacteriaiABI25781; ABI25781; HS_1508.
    GeneIDi4241028.
    KEGGihso:HS_1508.
    PATRICi20283147. VBIHaeSom53361_1584.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000436 Genomic DNA. Translation: ABI25781.1 .
    RefSeqi YP_719718.1. NC_008309.1.

    3D structure databases

    ProteinModelPortali Q0I5G9.
    SMRi Q0I5G9. Positions 36-826.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 205914.HS_1508.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABI25781 ; ABI25781 ; HS_1508 .
    GeneIDi 4241028.
    KEGGi hso:HS_1508.
    PATRICi 20283147. VBIHaeSom53361_1584.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci HSOM205914:GJ7V-1575-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd."
      Challacombe J.F., Duncan A.J., Brettin T.S., Bruce D., Chertkov O., Detter J.C., Han C.S., Misra M., Richardson P., Tapia R., Thayer N., Xie G., Inzana T.J.
      J. Bacteriol. 189:1890-1898(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 129Pt.

    Entry informationi

    Entry nameiNAPA_HISS1
    AccessioniPrimary (citable) accession number: Q0I5G9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: October 3, 2006
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3