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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Haemophilus somnus (strain 129Pt) (Histophilus somni)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi47Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi51Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi79Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:HS_1508
OrganismiHaemophilus somnus (strain 129Pt) (Histophilus somni)
Taxonomic identifieri205914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHistophilus
Proteomesi
  • UP000001970 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Tat-type signalUniRule annotationAdd BLAST32
ChainiPRO_100006972033 – 827Periplasmic nitrate reductaseAdd BLAST795

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi205914.HS_1508.

Structurei

3D structure databases

ProteinModelPortaliQ0I5G9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 934Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW. Bacteria.
COG0243. LUCA.
HOGENOMiHOG000031441.
KOiK02567.
OMAiCAVRIEL.
OrthoDBiPOG091H060P.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct. 1 hit.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10518. TAT_signal. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0I5G9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLSRRDFMK ANAALAAASV AGLIIPVKNV NAADTSITWD KAVCRFCGTG
60 70 80 90 100
CAVLVGTKDG RVVASQGDPD AEVNRGLNCI KGYFLPKIMY GKDRLTHPML
110 120 130 140 150
RMKNGQYDKE GEFTPVTWDF AFKTMAEKFK SALKAKGPNG VGMFTSGQST
160 170 180 190 200
IFEGVAKSKL FKAGLLSNNI DPNARHCMAS AAVAFVRTFG IDEPMGCYDD
210 220 230 240 250
IEHADAFVLW GSNMAEMHPI LWSRISDRRL ANPDTVSVNV LSTFEHRSFE
260 270 280 290 300
LADLGILLKP QSDLAILNYI ANYLIENNAI NREFIEKHTK FKRGETDIGY
310 320 330 340 350
GLRPQDPREQ IAKNVKTAGK MYDSSFEEFK KLVAPYTLEK AHEISGVPKE
360 370 380 390 400
QLEKLAKLYA DPNKKVVSYW TMGINQHTRG VWANHLIYNI HLLTGKISLP
410 420 430 440 450
GCGPFSLTGQ PSACGTAREV GTFIHRLPAD LVVTKPEHRK IAEKIWKLPE
460 470 480 490 500
GLISDKLGFH AVAQSRALKD GKMQVLWQMC NNNMQAGPNI NEETYPGWRN
510 520 530 540 550
PDNFIVVSDP YPTVSALSAD LILPTAMWVE KEGAYGNAER RTQFWRQQVK
560 570 580 590 600
APGEAKSDLW QLVEFSKYFT TDEVWPAEIL AKNPAYQGKT LYEVLYLNGQ
610 620 630 640 650
VNQYSNDELK GRLNDEAYHF GFYIQKGLFE EYASFGRGHG HDLADFDTYH
660 670 680 690 700
KARGLRWPVV DGKETLWRYR EGYDPYVKAG EGVSFYGQAD KRAVILAVPY
710 720 730 740 750
EPPAEVPDRE YDLWLTTGRI LEHWHTGSMT RRVPELHRSF PNNLVWMNPN
760 770 780 790 800
DAKKRGLKHG DKIKVISRRG EITSYIDTRG RNKCPEGLIY TTFFDAGQLA
810 820
NKLILDATDP ISKETDFKKC AVKVVKA
Length:827
Mass (Da):93,016
Last modified:October 3, 2006 - v1
Checksum:i19DCB138A81CCC7E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000436 Genomic DNA. Translation: ABI25781.1.
RefSeqiWP_011609659.1. NC_008309.1.

Genome annotation databases

EnsemblBacteriaiABI25781; ABI25781; HS_1508.
KEGGihso:HS_1508.
PATRICi20283147. VBIHaeSom53361_1584.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000436 Genomic DNA. Translation: ABI25781.1.
RefSeqiWP_011609659.1. NC_008309.1.

3D structure databases

ProteinModelPortaliQ0I5G9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi205914.HS_1508.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI25781; ABI25781; HS_1508.
KEGGihso:HS_1508.
PATRICi20283147. VBIHaeSom53361_1584.

Phylogenomic databases

eggNOGiENOG4107QIW. Bacteria.
COG0243. LUCA.
HOGENOMiHOG000031441.
KOiK02567.
OMAiCAVRIEL.
OrthoDBiPOG091H060P.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct. 1 hit.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10518. TAT_signal. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAPA_HAES1
AccessioniPrimary (citable) accession number: Q0I5G9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 3, 2006
Last modified: November 2, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.