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Q0I5G9

- NAPA_HISS1

UniProt

Q0I5G9 - NAPA_HISS1

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Protein
Periplasmic nitrate reductase
Gene
napA, HS_1508
Organism
Histophilus somni (strain 129Pt) (Haemophilus somnus)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441Iron-sulfur (4Fe-4S) By similarity
Metal bindingi47 – 471Iron-sulfur (4Fe-4S) By similarity
Metal bindingi51 – 511Iron-sulfur (4Fe-4S) By similarity
Metal bindingi79 – 791Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciHSOM205914:GJ7V-1575-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:HS_1508
OrganismiHistophilus somni (strain 129Pt) (Haemophilus somnus)
Taxonomic identifieri205914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHistophilus
ProteomesiUP000001970: Chromosome

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Tat-type signal Reviewed prediction
Add
BLAST
Chaini33 – 827795Periplasmic nitrate reductaseUniRule annotation
PRO_1000069720Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi205914.HS_1508.

Structurei

3D structure databases

ProteinModelPortaliQ0I5G9.
SMRiQ0I5G9. Positions 36-826.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 93574Fe-4S Mo/W bis-MGD-type
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0I5G9-1 [UniParc]FASTAAdd to Basket

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MNLSRRDFMK ANAALAAASV AGLIIPVKNV NAADTSITWD KAVCRFCGTG    50
CAVLVGTKDG RVVASQGDPD AEVNRGLNCI KGYFLPKIMY GKDRLTHPML 100
RMKNGQYDKE GEFTPVTWDF AFKTMAEKFK SALKAKGPNG VGMFTSGQST 150
IFEGVAKSKL FKAGLLSNNI DPNARHCMAS AAVAFVRTFG IDEPMGCYDD 200
IEHADAFVLW GSNMAEMHPI LWSRISDRRL ANPDTVSVNV LSTFEHRSFE 250
LADLGILLKP QSDLAILNYI ANYLIENNAI NREFIEKHTK FKRGETDIGY 300
GLRPQDPREQ IAKNVKTAGK MYDSSFEEFK KLVAPYTLEK AHEISGVPKE 350
QLEKLAKLYA DPNKKVVSYW TMGINQHTRG VWANHLIYNI HLLTGKISLP 400
GCGPFSLTGQ PSACGTAREV GTFIHRLPAD LVVTKPEHRK IAEKIWKLPE 450
GLISDKLGFH AVAQSRALKD GKMQVLWQMC NNNMQAGPNI NEETYPGWRN 500
PDNFIVVSDP YPTVSALSAD LILPTAMWVE KEGAYGNAER RTQFWRQQVK 550
APGEAKSDLW QLVEFSKYFT TDEVWPAEIL AKNPAYQGKT LYEVLYLNGQ 600
VNQYSNDELK GRLNDEAYHF GFYIQKGLFE EYASFGRGHG HDLADFDTYH 650
KARGLRWPVV DGKETLWRYR EGYDPYVKAG EGVSFYGQAD KRAVILAVPY 700
EPPAEVPDRE YDLWLTTGRI LEHWHTGSMT RRVPELHRSF PNNLVWMNPN 750
DAKKRGLKHG DKIKVISRRG EITSYIDTRG RNKCPEGLIY TTFFDAGQLA 800
NKLILDATDP ISKETDFKKC AVKVVKA 827
Length:827
Mass (Da):93,016
Last modified:October 3, 2006 - v1
Checksum:i19DCB138A81CCC7E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000436 Genomic DNA. Translation: ABI25781.1.
RefSeqiYP_719718.1. NC_008309.1.

Genome annotation databases

EnsemblBacteriaiABI25781; ABI25781; HS_1508.
GeneIDi4241028.
KEGGihso:HS_1508.
PATRICi20283147. VBIHaeSom53361_1584.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000436 Genomic DNA. Translation: ABI25781.1 .
RefSeqi YP_719718.1. NC_008309.1.

3D structure databases

ProteinModelPortali Q0I5G9.
SMRi Q0I5G9. Positions 36-826.
ModBasei Search...

Protein-protein interaction databases

STRINGi 205914.HS_1508.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABI25781 ; ABI25781 ; HS_1508 .
GeneIDi 4241028.
KEGGi hso:HS_1508.
PATRICi 20283147. VBIHaeSom53361_1584.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci HSOM205914:GJ7V-1575-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd."
    Challacombe J.F., Duncan A.J., Brettin T.S., Bruce D., Chertkov O., Detter J.C., Han C.S., Misra M., Richardson P., Tapia R., Thayer N., Xie G., Inzana T.J.
    J. Bacteriol. 189:1890-1898(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 129Pt.

Entry informationi

Entry nameiNAPA_HISS1
AccessioniPrimary (citable) accession number: Q0I5G9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 3, 2006
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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