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Q0I5C5 (SYL_HISS1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:HS_1557
OrganismHistophilus somni (strain 129Pt) (Haemophilus somnus) [Complete proteome] [HAMAP]
Taxonomic identifier205914 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHistophilus

Protein attributes

Sequence length860 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 860860Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009350

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif619 – 6235"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6221ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0I5C5 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: B5297172D5A0BEE0

FASTA86098,111
        10         20         30         40         50         60 
MQEQYRPDLI EADVQKYWAE KKTFKAVKDP SKEKYYCLSM FPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VISRYQRMNG KNVLQPMGWD AFGLPAEGAA IKNKTAPAKW TYENIEYMKN QLKVLGFGFD 

       130        140        150        160        170        180 
WDREITTCKP EYYKWEQWFF TELYKKGLVY KKTSTVNWCP NDETVLANEQ VHEGCCWRCD 

       190        200        210        220        230        240 
TPVEQKEIPQ WFIKITDYAE QLLSDLDQLP EWPDMVKTMQ RNWIGRSEGV EITFNVAHSD 

       250        260        270        280        290        300 
QTLTVYTTRP DTFYGVSYLA VAAAHPLAES AAKNNPELAA FIHEAKNTKV AEAELATMEK 

       310        320        330        340        350        360 
KGMATGLYAV HPMTGKQLPI WVANFVLMHY GTGAVMAVPA HDQRDYEFAQ KYQLPLFPVI 

       370        380        390        400        410        420 
KPADNSAWDF SKQAYTEHGI TINSAEFDGL DFEATFNGIA DKLEKIGVGK RQVNYRLRDW 

       430        440        450        460        470        480 
GVSRQRYWGA PIPMLTLENG DVVVAPLQDL PIVLPEDVVM DGVKSPIKAD PEWAKTTYNG 

       490        500        510        520        530        540 
QAALKETDTF DTFMESSWYY ARYTSPQYQQ AMLDADEANY WLPVDQYIGG IEHATMHLLY 

       550        560        570        580        590        600 
FRFFHKLLRD AGFVTSDEPS KKLLCQGMVL ADAFYYTSPT NERIWVSPTK VTLERDEKGR 

       610        620        630        640        650        660 
IIKAVDDEGH ELVHSGMTKM SKSKNNGIDP QEMVEKYGAD TVRLFMMFAS PAEMTLEWQE 

       670        680        690        700        710        720 
SGVEGANRFL RRLWNLVFEY NQNPAQTALD PTALSVEQKA LRRDVHKTIA KVSDDIGRRQ 

       730        740        750        760        770        780 
TFNTAIAAIM ELMNKLTKAS LSNEQDRAVM AEALNAVVRM LYPITPHICF QLWQDLGNES 

       790        800        810        820        830        840 
TIDFAPWVIA DAEAMVEDEK LVVIQVNGKV RAKVTVPADM SEDEIKQVAL AEENVQKFLN 

       850        860 
GLTVVKTIYV QGKLFSFVAK 

« Hide

References

[1]"Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd."
Challacombe J.F., Duncan A.J., Brettin T.S., Bruce D., Chertkov O., Detter J.C., Han C.S., Misra M., Richardson P., Tapia R., Thayer N., Xie G., Inzana T.J.
J. Bacteriol. 189:1890-1898(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 129Pt.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000436 Genomic DNA. Translation: ABI25825.1.
RefSeqYP_719762.1. NC_008309.1.

3D structure databases

ProteinModelPortalQ0I5C5.
SMRQ0I5C5. Positions 228-413.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING205914.HS_1557.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI25825; ABI25825; HS_1557.
GeneID4241078.
KEGGhso:HS_1557.
PATRIC20283253. VBIHaeSom53361_1633.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycHSOM205914:GJ7V-1628-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_HISS1
AccessionPrimary (citable) accession number: Q0I5C5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: May 14, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries