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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Haemophilus somnus (strain 129Pt) (Histophilus somni)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei622 – 6221ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHSOM205914:GJ7V-1628-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:HS_1557
OrganismiHaemophilus somnus (strain 129Pt) (Histophilus somni)
Taxonomic identifieri205914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHistophilus
ProteomesiUP000001970 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 860860Leucine--tRNA ligasePRO_1000009350Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi205914.HS_1557.

Structurei

3D structure databases

ProteinModelPortaliQ0I5C5.
SMRiQ0I5C5. Positions 228-413.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi619 – 6235"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0I5C5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEQYRPDLI EADVQKYWAE KKTFKAVKDP SKEKYYCLSM FPYPSGRLHM
60 70 80 90 100
GHVRNYTIGD VISRYQRMNG KNVLQPMGWD AFGLPAEGAA IKNKTAPAKW
110 120 130 140 150
TYENIEYMKN QLKVLGFGFD WDREITTCKP EYYKWEQWFF TELYKKGLVY
160 170 180 190 200
KKTSTVNWCP NDETVLANEQ VHEGCCWRCD TPVEQKEIPQ WFIKITDYAE
210 220 230 240 250
QLLSDLDQLP EWPDMVKTMQ RNWIGRSEGV EITFNVAHSD QTLTVYTTRP
260 270 280 290 300
DTFYGVSYLA VAAAHPLAES AAKNNPELAA FIHEAKNTKV AEAELATMEK
310 320 330 340 350
KGMATGLYAV HPMTGKQLPI WVANFVLMHY GTGAVMAVPA HDQRDYEFAQ
360 370 380 390 400
KYQLPLFPVI KPADNSAWDF SKQAYTEHGI TINSAEFDGL DFEATFNGIA
410 420 430 440 450
DKLEKIGVGK RQVNYRLRDW GVSRQRYWGA PIPMLTLENG DVVVAPLQDL
460 470 480 490 500
PIVLPEDVVM DGVKSPIKAD PEWAKTTYNG QAALKETDTF DTFMESSWYY
510 520 530 540 550
ARYTSPQYQQ AMLDADEANY WLPVDQYIGG IEHATMHLLY FRFFHKLLRD
560 570 580 590 600
AGFVTSDEPS KKLLCQGMVL ADAFYYTSPT NERIWVSPTK VTLERDEKGR
610 620 630 640 650
IIKAVDDEGH ELVHSGMTKM SKSKNNGIDP QEMVEKYGAD TVRLFMMFAS
660 670 680 690 700
PAEMTLEWQE SGVEGANRFL RRLWNLVFEY NQNPAQTALD PTALSVEQKA
710 720 730 740 750
LRRDVHKTIA KVSDDIGRRQ TFNTAIAAIM ELMNKLTKAS LSNEQDRAVM
760 770 780 790 800
AEALNAVVRM LYPITPHICF QLWQDLGNES TIDFAPWVIA DAEAMVEDEK
810 820 830 840 850
LVVIQVNGKV RAKVTVPADM SEDEIKQVAL AEENVQKFLN GLTVVKTIYV
860
QGKLFSFVAK
Length:860
Mass (Da):98,111
Last modified:October 3, 2006 - v1
Checksum:iB5297172D5A0BEE0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000436 Genomic DNA. Translation: ABI25825.1.
RefSeqiWP_011609703.1. NC_008309.1.

Genome annotation databases

EnsemblBacteriaiABI25825; ABI25825; HS_1557.
KEGGihso:HS_1557.
PATRICi20283253. VBIHaeSom53361_1633.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000436 Genomic DNA. Translation: ABI25825.1.
RefSeqiWP_011609703.1. NC_008309.1.

3D structure databases

ProteinModelPortaliQ0I5C5.
SMRiQ0I5C5. Positions 228-413.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi205914.HS_1557.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI25825; ABI25825; HS_1557.
KEGGihso:HS_1557.
PATRICi20283253. VBIHaeSom53361_1633.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciHSOM205914:GJ7V-1628-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd."
    Challacombe J.F., Duncan A.J., Brettin T.S., Bruce D., Chertkov O., Detter J.C., Han C.S., Misra M., Richardson P., Tapia R., Thayer N., Xie G., Inzana T.J.
    J. Bacteriol. 189:1890-1898(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 129Pt.

Entry informationi

Entry nameiSYL_HAES1
AccessioniPrimary (citable) accession number: Q0I5C5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: July 22, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.