Q0I554 (PCKA_HAES1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate carboxykinase [ATP]
Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.49

Alternative name(s):
Phosphoenolpyruvate carboxylase

Gene names

Name:	pckA
Ordered Locus Names:	HS_1628

Organism

Haemophilus somnus (strain 129Pt) (Histophilus somni (strain 129Pt)) [Complete proteome] [HAMAP]

Taxonomic identifier

205914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Histophilus

Protein attributes

Sequence length	538 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2. HAMAP MF_00453
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00453
Subcellular location	Cytoplasm By similarity HAMAP MF_00453.
Sequence similarities	Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoenolpyruvate carboxykinase (ATP) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 538	538	Phosphoenolpyruvate carboxykinase [ATP] HAMAP MF_00453		PRO_1000026329
Regions
	Nucleotide binding	246 – 253	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0I554 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 9B41B8E1542E7E5D	FASTA	538	59,067
	10 20 30 40 50 60 MTDLNKVINE LGELGIYDVK EIVHNPSYEQ LFEEETKPGL AGFEKGVVTT SGAVAVDTGI 70 80 90 100 110 120 FTGRSPKDKY IVLDEKTKNT VWWTSDAAKN DNKPMNQETW QSLKGLVTKQ LSGKRLFVID 130 140 150 160 170 180 AFCGANPDTR LAVRIVTEVA WQAHFVKNMF IRPSDEELQN FKPDFTVMNG SKVTNPNWKE 190 200 210 220 230 240 QGLNSENFVA FNITEGVQLI GGTWYGGEMK KGMFSMMNYF LPLKGIASMH CSANVGKDGD 250 260 270 280 290 300 VAIFFGLSGT GKTTLSTDPK RQLIGDDEHG WDDDGVFNYE GGCYAKTIKL SAESEPDIYR 310 320 330 340 350 360 AIRRDALLEN VVVREDGSVD FDDGSKTENT RVSYPIYHID NIVKPVSKAG HATKVIFLTA 370 380 390 400 410 420 DAFGVLPPVS KLTPEQTKYY FLSGFTAKLA GTERGITEPT PTFSACFGAA FLSLHPTQYA 430 440 450 460 470 480 EVLVKRMKDS GAEAYLVNTG WNGTGKRISI KDTRGIIDAI LDGSIESAEM GSLPIFDLAI 490 500 510 520 530 PKALPGVDPA ILDPRDTYAD KAQWQAKAED LASRFVKNFE KYATNEEGKA LIAAGPKA

References

[1]

"Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd." Challacombe J.F., Duncan A.J., Brettin T.S., Bruce D., Chertkov O., Detter J.C., Han C.S., Misra M., Richardson P., Tapia R., Thayer N., Xie G., Inzana T.J. J. Bacteriol. 189:1890-1898(2007) [PubMed: 17172329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 129Pt.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000436 Genomic DNA. Translation: ABI25896.1.
RefSeq	YP_719833.1. NC_008309.1.
3D structure databases
ProteinModelPortal	Q0I554.
SMR	Q0I554. Positions 3-537.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0I554.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4241155.
GenomeReviews	Gene locus HS_1628 in contig CP000436_GR.
KEGG	hso:HS_1628.
NMPDR	fig|205914.1.peg.1617.
PATRIC	20283413. VBIHaeSom53361_1706.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1866.
HOGENOM	HBG293387.
OMA	VYNPSYE.
PhylomeDB	Q0I554.
ProtClustDB	PRK09344.
Enzyme and pathway databases
BioCyc	HSOM205914:HS_1628-MONOMER.
Family and domain databases
HAMAP	MF_00453. PEPCK_ATP. [Tree]
InterPro	IPR001272. PEP_carboxykinase_ATP. IPR013035. PEP_carboxykinase_C. IPR008210. PEP_carboxykinase_N. IPR015994. PEPCK_ATP_CS. [Graphical view]
Gene3D	G3DSA:3.90.228.20. PEP_carboxykinase_C. 2 hits. G3DSA:3.40.449.10. PEP_carboxykinase_N. 1 hit.
KO	K01610.
Pfam	PF01293. PEPCK_ATP. 1 hit. [Graphical view]
PIRSF	PIRSF006294. PEP_crbxkin. 1 hit.
SUPFAM	SSF68923. PEP_carboxykinase_N. 1 hit.
TIGRFAMs	TIGR00224. PckA. 1 hit.
PROSITE	PS00532. PEPCK_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCKA_HAES1

Accession

Primary (citable) accession number: Q0I554

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 3, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program