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Q0I516 (TRMB_HISS1) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:HS_1666
OrganismHistophilus somni (strain 129Pt) (Haemophilus somnus) [Complete proteome] [HAMAP]
Taxonomic identifier205914 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHistophilus

Protein attributes

Sequence length251 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 251251tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288154

Regions

Region228 – 2314Substrate binding By similarity

Sites

Active site1551 By similarity
Binding site801S-adenosyl-L-methionine By similarity
Binding site1051S-adenosyl-L-methionine By similarity
Binding site1321S-adenosyl-L-methionine By similarity
Binding site1551S-adenosyl-L-methionine By similarity
Binding site1591Substrate By similarity
Binding site1911Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0I516 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: FBFA01B7AD17A7C4

FASTA25128,967
        10         20         30         40         50         60 
MTEQKITFAD QKRKTVEIAE FTEDGRYKRK VRSFVLRTGR LSEFQRNMMN NNWATLGLEY 

        70         80         90        100        110        120 
QTTAFDFTQI YGNTNPVILE IGFGMGKSLV EMALQNPDKN YLGIEVHTPG VGACIAYAVE 

       130        140        150        160        170        180 
KQVKNLRVIC HDATEILQDC IADNSLAGLQ LFFPDPWHKT KHHKRRIVQP HFVEKIQQKL 

       190        200        210        220        230        240 
VPNGFVHMAT DWENYAEYML EVLTSAVGLR NTSATNDYIP RPDFRPLTKF EQRGHKLGHG 

       250 
VWDLFFIKNI T 

« Hide

References

[1]"Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd."
Challacombe J.F., Duncan A.J., Brettin T.S., Bruce D., Chertkov O., Detter J.C., Han C.S., Misra M., Richardson P., Tapia R., Thayer N., Xie G., Inzana T.J.
J. Bacteriol. 189:1890-1898(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 129Pt.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000436 Genomic DNA. Translation: ABI25934.1.
RefSeqYP_719871.1. NC_008309.1.

3D structure databases

ProteinModelPortalQ0I516.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING205914.HS_1666.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI25934; ABI25934; HS_1666.
GeneID4241193.
KEGGhso:HS_1666.
PATRIC20283489. VBIHaeSom53361_1744.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycHSOM205914:GJ7V-1747-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_HISS1
AccessionPrimary (citable) accession number: Q0I516
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 3, 2006
Last modified: June 11, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways