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Protein

Argininosuccinate synthase

Gene

argG

Organism
Haemophilus somnus (strain 129Pt) (Histophilus somni)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathway: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441ATPUniRule annotation
Binding sitei100 – 1001CitrullineUniRule annotation
Binding sitei130 – 1301ATP; via amide nitrogenUniRule annotation
Binding sitei132 – 1321AspartateUniRule annotation
Binding sitei132 – 1321ATPUniRule annotation
Binding sitei136 – 1361AspartateUniRule annotation
Binding sitei136 – 1361CitrullineUniRule annotation
Binding sitei137 – 1371AspartateUniRule annotation
Binding sitei137 – 1371ATPUniRule annotation
Binding sitei140 – 1401CitrullineUniRule annotation
Binding sitei193 – 1931CitrullineUniRule annotation
Binding sitei195 – 1951ATPUniRule annotation
Binding sitei202 – 2021CitrullineUniRule annotation
Binding sitei204 – 2041CitrullineUniRule annotation
Binding sitei281 – 2811CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 269ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHSOM205914:GJ7V-1421-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:HS_1362
OrganismiHaemophilus somnus (strain 129Pt) (Histophilus somni)
Taxonomic identifieri205914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHistophilus
ProteomesiUP000001970 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444Argininosuccinate synthasePRO_1000025428Add
BLAST

Proteomic databases

PRIDEiQ0I4M1.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi205914.HS_1362.

Structurei

3D structure databases

ProteinModelPortaliQ0I4M1.
SMRiQ0I4M1. Positions 4-442.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230094.
KOiK01940.
OMAiMRNLDIA.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0I4M1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNTILQHLP IGQKVGIAFS GGLDTSAALL WMRQKGAVPY AYTANLGQPD
60 70 80 90 100
EEDYNAIPRK AMEYGAEKAR LIDCRNQLAH EGIAAIQCGA FHISTGGVTY
110 120 130 140 150
FNTTPLGRAV TGTMLVSAMK EDDVNIWGDG STFKGNDIER FYRYGLLTNP
160 170 180 190 200
SLRIYKPWLD NQFIDELGGR QEMSEFLIAN GFDYKMSVEK AYSTDSNMLG
210 220 230 240 250
ATHEAKDLEY LNSGIRIVKP IMGVAFWRDD VTVKAEEVTV GFEDGVPVTL
260 270 280 290 300
NGQSFSSAVE LFLEANRIGG RHGLGMSDQI ENRIIEAKSR GIYEAPGMAL
310 320 330 340 350
LHIAYERLVT AIHNEDTIEQ YRINGLRLGR LLYQGRWFDP QALMLRETAQ
360 370 380 390 400
RWVARAVTGE VTLELRRGND YSILNTVSPN MTYHPERLSM EKVENAPFDP
410 420 430 440
SDRIGQLTMR NLDITDTRDK LGIYTHTGLL TVGKDSMLPQ LDKK
Length:444
Mass (Da):49,635
Last modified:October 3, 2006 - v1
Checksum:iE6A7CDA785070D5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000436 Genomic DNA. Translation: ABI25637.1.
RefSeqiWP_011609516.1. NC_008309.1.
YP_719574.1. NC_008309.1.

Genome annotation databases

EnsemblBacteriaiABI25637; ABI25637; HS_1362.
KEGGihso:HS_1362.
PATRICi20282831. VBIHaeSom53361_1432.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000436 Genomic DNA. Translation: ABI25637.1.
RefSeqiWP_011609516.1. NC_008309.1.
YP_719574.1. NC_008309.1.

3D structure databases

ProteinModelPortaliQ0I4M1.
SMRiQ0I4M1. Positions 4-442.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi205914.HS_1362.

Proteomic databases

PRIDEiQ0I4M1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI25637; ABI25637; HS_1362.
KEGGihso:HS_1362.
PATRICi20282831. VBIHaeSom53361_1432.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230094.
KOiK01940.
OMAiMRNLDIA.
OrthoDBiEOG6K9QCV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciHSOM205914:GJ7V-1421-MONOMER.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd."
    Challacombe J.F., Duncan A.J., Brettin T.S., Bruce D., Chertkov O., Detter J.C., Han C.S., Misra M., Richardson P., Tapia R., Thayer N., Xie G., Inzana T.J.
    J. Bacteriol. 189:1890-1898(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 129Pt.

Entry informationi

Entry nameiASSY_HAES1
AccessioniPrimary (citable) accession number: Q0I4M1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: June 24, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.