Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-hexosaminidase

Gene

nagZ

Organism
Histophilus somni (strain 129Pt) (Haemophilus somnus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides.UniRule annotation

Catalytic activityi

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei62 – 621SubstrateUniRule annotation
Binding sitei70 – 701SubstrateUniRule annotation
Binding sitei134 – 1341SubstrateUniRule annotation
Sitei175 – 1751Important for catalytic activityUniRule annotation
Active sitei177 – 1771Proton donor/acceptorUniRule annotation
Active sitei249 – 2491NucleophileUniRule annotation

GO - Molecular functioni

  1. beta-N-acetylhexosaminidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. cell cycle Source: UniProtKB-KW
  3. cell division Source: UniProtKB-KW
  4. cell wall organization Source: UniProtKB-KW
  5. peptidoglycan biosynthetic process Source: UniProtKB-KW
  6. peptidoglycan turnover Source: UniProtKB-HAMAP
  7. regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciHSOM205914:GJ7V-1174-MONOMER.
UniPathwayiUPA00544.

Protein family/group databases

CAZyiGH3. Glycoside Hydrolase Family 3.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-hexosaminidaseUniRule annotation (EC:3.2.1.52UniRule annotation)
Alternative name(s):
Beta-N-acetylhexosaminidaseUniRule annotation
N-acetyl-beta-glucosaminidaseUniRule annotation
Gene namesi
Name:nagZUniRule annotation
Ordered Locus Names:HS_1132
OrganismiHistophilus somni (strain 129Pt) (Haemophilus somnus)
Taxonomic identifieri205914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHistophilus
ProteomesiUP000001970: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 351351Beta-hexosaminidasePRO_1000005655Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi205914.HS_1132.

Structurei

3D structure databases

ProteinModelPortaliQ0I414.
SMRiQ0I414. Positions 3-301.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni164 – 1652Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1472.
HOGENOMiHOG000248526.
KOiK01207.
OMAiAGFSSYW.
OrthoDBiEOG6BCT06.

Family and domain databases

Gene3Di3.20.20.300. 1 hit.
HAMAPiMF_00364. NagZ.
InterProiIPR022956. Beta_hexosaminidase_bac.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00933. Glyco_hydro_3. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0I414-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSILLIDLSS QELRQEEIEL LEHPLVAGLI LFSRNFYDIE QIRHLIRSVR
60 70 80 90 100
QKVKKPLLIT VDQEGGRVQR FRQGFTQLPA MQSFACLLSD PQEQQEMAWR
110 120 130 140 150
AGWQMAAEMT ALDIDLSFAP VLDLGHQCKA IGDRSFHYEE KKLIELAEKF
160 170 180 190 200
IQGMRQIGMS ATGKHFPGHG HVLADSHLET PYDDRAKELI FAQDIRPFQS
210 220 230 240 250
LIKQGLLDAV MPAHVIYTQC DNQPASGSSY WLKEVLRQQL GFQGAIFSDD
260 270 280 290 300
LGMKGAGFMG DFVARCTQSL QAGCDLLLLC NEPEAVVQVL DRFKPQESQN
310 320 330 340 350
KRIIRQTRLN KLFKKQRIDW QTLRNQRDWL ENHKKLTALQ QDWLAYKGYD

N
Length:351
Mass (Da):40,356
Last modified:October 3, 2006 - v1
Checksum:i7304D5BA829F4960
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000436 Genomic DNA. Translation: ABI25407.1.
RefSeqiYP_719344.1. NC_008309.1.

Genome annotation databases

EnsemblBacteriaiABI25407; ABI25407; HS_1132.
GeneIDi4240633.
KEGGihso:HS_1132.
PATRICi20282311. VBIHaeSom53361_1186.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000436 Genomic DNA. Translation: ABI25407.1.
RefSeqiYP_719344.1. NC_008309.1.

3D structure databases

ProteinModelPortaliQ0I414.
SMRiQ0I414. Positions 3-301.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi205914.HS_1132.

Protein family/group databases

CAZyiGH3. Glycoside Hydrolase Family 3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI25407; ABI25407; HS_1132.
GeneIDi4240633.
KEGGihso:HS_1132.
PATRICi20282311. VBIHaeSom53361_1186.

Phylogenomic databases

eggNOGiCOG1472.
HOGENOMiHOG000248526.
KOiK01207.
OMAiAGFSSYW.
OrthoDBiEOG6BCT06.

Enzyme and pathway databases

UniPathwayiUPA00544.
BioCyciHSOM205914:GJ7V-1174-MONOMER.

Family and domain databases

Gene3Di3.20.20.300. 1 hit.
HAMAPiMF_00364. NagZ.
InterProiIPR022956. Beta_hexosaminidase_bac.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00933. Glyco_hydro_3. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd."
    Challacombe J.F., Duncan A.J., Brettin T.S., Bruce D., Chertkov O., Detter J.C., Han C.S., Misra M., Richardson P., Tapia R., Thayer N., Xie G., Inzana T.J.
    J. Bacteriol. 189:1890-1898(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 129Pt.

Entry informationi

Entry nameiNAGZ_HISS1
AccessioniPrimary (citable) accession number: Q0I414
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: March 4, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.