Reviewed, UniProtKB/Swiss-Prot Q0I3Q7 (GLO2_HAES1)

Names and origin

Protein names

Recommended name:
    Hydroxyacylglutathione hydrolase
    EC=3.1.2.6
Alternative name(s):
    Glyoxalase II
      Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	HS_0809

Organism

Haemophilus somnus (strain 129Pt) (Histophilus somni (strain 129Pt)) [Complete proteome] [HAMAP]

Taxonomic identifier

205914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Histophilus

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 233	233	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_0000309650
Sites
	Metal binding	52	1	Zinc 1 By similarity
	Metal binding	54	1	Zinc 1 By similarity
	Metal binding	56	1	Zinc 2 By similarity
	Metal binding	57	1	Zinc 2 By similarity
	Metal binding	108	1	Zinc 1 By similarity
	Metal binding	125	1	Zinc 1 By similarity
	Metal binding	125	1	Zinc 2 By similarity
	Metal binding	163	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0I3Q7-1 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: AAC73C93A9C3A536	FASTA	233	25,965
	10 20 30 40 50 60 MLIPIPALND NYIWVYKREN LSVIVIDIPE IAALSQFIQA QNLVIGALLI THNHRDHIGA 70 80 90 100 110 120 LAEFKQFYPH VHIYGPAECA DQGVTQVIDA GRLIIDEYHI DVLPTGGHTA QHLSFLIDGH 130 140 150 160 170 180 LFCGDTLFSA GCGRVFTGDY SQMFTSLQLL KSLPDETIVC PAHEYTLGNL AFALTTGKNK 190 200 210 220 230 SAVQKQLEKV KKLRAENKPS LPTTLALEKQ INPFLQVESL DEFIELRKAK DVF

References

[1]

"Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd." Challacombe J.F., Duncan A.J., Brettin T.S., Bruce D., Chertkov O., Detter J.C., Han C.S., Misra M., Richardson P., Tapia R., Thayer N., Xie G., Inzana T.J. J. Bacteriol. 189:1890-1898(2007) [PubMed: 17172329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000436 Genomic DNA. Translation: ABI25084.1.
RefSeq	YP_719019.1.
3D structure databases
SMR	Q0I3Q7. Positions 2-233.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0I3Q7.
Genome annotation databases
GeneID	4240300.
GenomeReviews	Gene locus HS_0809 in contig CP000436_GR.
KEGG	hso:HS_0809.
NMPDR	fig|205914.1.peg.310.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0491.
HOGENOM	HBG753931.
OMA	WCAHEYT.
PhylomeDB	Q0I3Q7.
Enzyme and pathway databases
BioCyc	HSOM205914:HS_0809-MONOMER.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Blactmase-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_HAES1

Accession

Primary (citable) accession number: Q0I3Q7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	October 3, 2006
Last modified:	February 9, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)