Q0I3P2 (METE_HAES1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	HS_0824

Organism

Haemophilus somnus (strain 129Pt) (Histophilus somni (strain 129Pt)) [Complete proteome] [HAMAP]

Taxonomic identifier

205914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Histophilus

Protein attributes

Sequence length	757 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 757	757	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017247
Sites
	Metal binding	643	1	Zinc By similarity
	Metal binding	645	1	Zinc By similarity
	Metal binding	728	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0I3P2 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 7DB7AADB2887E374	FASTA	757	85,725
	10 20 30 40 50 60 MTTFHVLGFP RVGAKRELKF AQERYWRKEL AEQDLLDLAK ALREKNWKHQ ADANADFVAV 70 80 90 100 110 120 GDFTFYDHIL DLQVATGAIP ARFGFDSKNL TLDQYFQLAR GNKDQFAIEM TKWFDTNYHY 130 140 150 160 170 180 LVPEFHKDTQ FKANPTHYVN QIREAKVAGH QVKPVIVGPL TFLWLGKEKG ESFNRFELLA 190 200 210 220 230 240 QLVPVYVEIL NALVAEGAEW IQIDEPALAV DLPKEWGNAY KDVYATLSEK VNAKLLLATY 250 260 270 280 290 300 FGSVTEHATL LKELSVDGLH LDLVRAPEQL VAFEDYNKVL SAGVIDGRNI WRANLNTVLD 310 320 330 340 350 360 VLEPLKEKLG ERLWIAPSCS LLHTPFDLNV ETQLQENNPA LYSWLAFTLQ KVQELSVLKT 370 380 390 400 410 420 ALEKGRTAVN VELDASQVAA DARANSKEIH RPEVAERLAN LPKNADQRKS PFGERIKLQN 430 440 450 460 470 480 AWLNLPLLPT TSIGSFPQTT DIRRARAAFK KGDLSLEEYE NSMKKEIELV VREQEKLDLD 490 500 510 520 530 540 VLVHGEPERN DMVEYFGELL DGFAFTKFGW VQSYGSRCVK PPVIYGDVVR PKAMTVRWSK 550 560 570 580 590 600 YAQSLTKKVM KGMLTGPVTI LQWSFVRNDI PRSTVCKQIG VALSDEVLDL EKAGIKVIQI 610 620 630 640 650 660 DEPAIREGLP LKRADWDTYL QWAGEAFRLS SMGVADDTQI HTHMCYSEFN DILPAIAALD 670 680 690 700 710 720 ADVITIETSR SDMELLTAFG DFKYPNDIGP GVYDIHSPRV PTAEEIEHLL RKALNVVPKE 730 740 750 RLWVNPDCGL KTRGWSETIA QLEVMMSVTK KLRAELA

References

[1]

"Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd." Challacombe J.F., Duncan A.J., Brettin T.S., Bruce D., Chertkov O., Detter J.C., Han C.S., Misra M., Richardson P., Tapia R., Thayer N., Xie G., Inzana T.J. J. Bacteriol. 189:1890-1898(2007) [PubMed: 17172329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 129Pt.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000436 Genomic DNA. Translation: ABI25099.1.
RefSeq	YP_719034.1. NC_008309.1.
3D structure databases
ProteinModelPortal	Q0I3P2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0I3P2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4240316.
GenomeReviews	Gene locus HS_0824 in contig CP000436_GR.
KEGG	hso:HS_0824.
NMPDR	fig|205914.1.peg.295.
PATRIC	20281657. VBIHaeSom53361_0865.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HBG287495.
OMA	RNIWRAN.
PhylomeDB	Q0I3P2.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	HSOM205914:HS_0824-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_HAES1

Accession

Primary (citable) accession number: Q0I3P2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 3, 2006
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program