Q0I3G4 (THII_HAES1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA sulfurtransferase
EC=2.8.1.4

Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferase
Thiamine biosynthesis protein ThiI
tRNA 4-thiouridine synthase

Gene names

Name:	thiI
Ordered Locus Names:	HS_0902

Organism

Haemophilus somnus (strain 129Pt) (Histophilus somni (strain 129Pt)) [Complete proteome] [HAMAP]

Taxonomic identifier

205914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Histophilus › Haemophilus somnus

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS By similarity. HAMAP-Rule MF_00021
Catalytic activity	L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide. HAMAP-Rule MF_00021 [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP. HAMAP-Rule MF_00021
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis. HAMAP-Rule MF_00021
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00021.
Sequence similarities	Belongs to the ThiI family. Contains 1 rhodanese domain. Contains 1 THUMP domain.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Transferase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA thio-modification Inferred from electronic annotation. Source: HAMAP thiamine biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamine diphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway thiazole biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP sulfurtransferase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	tRNA sulfurtransferase HAMAP-Rule MF_00021		PRO_1000074229
Regions
	Domain	61 – 165	105	THUMP
	Domain	404 – 484	81	Rhodanese
	Nucleotide binding	183 – 184	2	ATP By similarity
Sites
	Active site	456	1	Cysteine persulfide intermediate By similarity
	Binding site	265	1	ATP By similarity
	Binding site	287	1	ATP; via amide nitrogen By similarity
	Binding site	296	1	ATP By similarity
Amino acid modifications
	Disulfide bond	344 ↔ 456		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0I3G4 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 8D24364396FA415B	FASTA	484	54,756
	10 20 30 40 50 60 MKFIIKLFPE IMIKSESVRK RFVKILTGNI RNVLNKYDDT VAVVKHWDYI EVRSKNIENR 70 80 90 100 110 120 ILLVELLGRI PGIHHFLEVE EKPFVTLHDI FEQTLSDVAT QIENKTFCVR VKRKGKHDFS 130 140 150 160 170 180 SLDAERYIGG GLNQAVASAK VQLSKPDVTV RIDIENDKMM LIKARHQGIG GYPIGTQEDV 190 200 210 220 230 240 LSLISGGFDS GVSSYMLIRR GSRVHYCFFN LGGATHEIGV KQMAYHIWKR FSGSHKVRFV 250 260 270 280 290 300 AINFEQVVAE ILEKVDNGQM GVVLKRMMVR AASKVAQRFG IQAIVTGEAL GQVSSQTLTN 310 320 330 340 350 360 LRLIDEAAES LVLRPLITHD KEQIIAKAKE IGTEDIAKSM PEFCGVISKS PTVKAVKEKI 370 380 390 400 410 420 EQEESYFDFS VLESAVQNAQ YLDIRQIAEQ TKKEVFEVDE ITVLSANDVI LDIRSPEEVD 430 440 450 460 470 480 DKPLEISGQN IILMPFYKLS SHFAELDQSK NYVLYCERGV MSKLQALYLR EKGFDNVKVL NKIS

References

[1]

"Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd." Challacombe J.F., Duncan A.J., Brettin T.S., Bruce D., Chertkov O., Detter J.C., Han C.S., Misra M., Richardson P., Tapia R., Thayer N., Xie G., Inzana T.J. J. Bacteriol. 189:1890-1898(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 129Pt.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000436 Genomic DNA. Translation: ABI25177.1.
RefSeq	YP_719112.1. NC_008309.1.
3D structure databases
ProteinModelPortal	Q0I3G4.
ModBase	Search...
Protein-protein interaction databases
STRING	205914.HS_0902.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABI25177; ABI25177; HS_0902.
GeneID	4240394.
KEGG	hso:HS_0902.
PATRIC	20281817. VBIHaeSom53361_0945.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0301.
HOGENOM	HOG000227469.
KO	K03151.
OMA	KLFPEIM.
ProtClustDB	PRK01269.
Enzyme and pathway databases
BioCyc	HSOM205914:GJ7V-935-MONOMER.
UniPathway	UPA00060.
Family and domain databases
Gene3D	3.40.250.10. 1 hit. 3.40.50.620. 1 hit.
HAMAP	MF_00021. ThiI.
InterPro	IPR001763. Rhodanese-like_dom. IPR014729. Rossmann-like_a/b/a_fold. IPR026340. Thiazole_biosynth_dom. IPR003720. ThiI. IPR020536. ThiI_C_dom. IPR004114. THUMP. [Graphical view]
Pfam	PF02568. ThiI. 1 hit. PF02926. THUMP. 1 hit. [Graphical view]
SMART	SM00981. THUMP. 1 hit. [Graphical view]
SUPFAM	SSF52821. Rhodanese-like. 1 hit.
TIGRFAMs	TIGR04271. ThiI_C_thiazole. 1 hit. TIGR00342. TIGR00342. 1 hit.
PROSITE	PS50206. RHODANESE_3. 1 hit. PS51165. THUMP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THII_HAES1

Accession

Primary (citable) accession number: Q0I3G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 3, 2006
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program