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Q0I3G4

- THII_HISS1

UniProt

Q0I3G4 - THII_HISS1

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Protein

tRNA sulfurtransferase

Gene

thiI

Organism
Histophilus somni (strain 129Pt) (Haemophilus somnus)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotation

Catalytic activityi

L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotation
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei265 – 2651ATPUniRule annotation
Binding sitei287 – 2871ATP; via amide nitrogenUniRule annotation
Binding sitei296 – 2961ATPUniRule annotation
Active sitei456 – 4561Cysteine persulfide intermediateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi183 – 1842ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. sulfurtransferase activity Source: UniProtKB-HAMAP
  3. tRNA adenylyltransferase activity Source: InterPro
  4. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-HAMAP
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
  3. thiazole biosynthetic process Source: InterPro
  4. tRNA thio-modification Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciHSOM205914:GJ7V-935-MONOMER.
UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
Thiamine biosynthesis protein ThiIUniRule annotation
tRNA 4-thiouridine synthaseUniRule annotation
Gene namesi
Name:thiIUniRule annotation
Ordered Locus Names:HS_0902
OrganismiHistophilus somni (strain 129Pt) (Haemophilus somnus)
Taxonomic identifieri205914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHistophilus
ProteomesiUP000001970: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 484484tRNA sulfurtransferasePRO_1000074229Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi344 ↔ 456Redox-activeUniRule annotation

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi205914.HS_0902.

Structurei

3D structure databases

ProteinModelPortaliQ0I3G4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini61 – 165105THUMPUniRule annotationAdd
BLAST
Domaini404 – 48481RhodaneseUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ThiI family.UniRule annotation
Contains 1 rhodanese domain.UniRule annotation
Contains 1 THUMP domain.UniRule annotation

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiCOG0301.
HOGENOMiHOG000227469.
KOiK03151.
OrthoDBiEOG6TBHGR.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI.
InterProiIPR001763. Rhodanese-like_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR026340. Thiazole_biosynth_dom.
IPR020536. ThiI_AANH.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamiPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
TIGRFAMsiTIGR04271. ThiI_C_thiazole. 1 hit.
TIGR00342. TIGR00342. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS51165. THUMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0I3G4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFIIKLFPE IMIKSESVRK RFVKILTGNI RNVLNKYDDT VAVVKHWDYI
60 70 80 90 100
EVRSKNIENR ILLVELLGRI PGIHHFLEVE EKPFVTLHDI FEQTLSDVAT
110 120 130 140 150
QIENKTFCVR VKRKGKHDFS SLDAERYIGG GLNQAVASAK VQLSKPDVTV
160 170 180 190 200
RIDIENDKMM LIKARHQGIG GYPIGTQEDV LSLISGGFDS GVSSYMLIRR
210 220 230 240 250
GSRVHYCFFN LGGATHEIGV KQMAYHIWKR FSGSHKVRFV AINFEQVVAE
260 270 280 290 300
ILEKVDNGQM GVVLKRMMVR AASKVAQRFG IQAIVTGEAL GQVSSQTLTN
310 320 330 340 350
LRLIDEAAES LVLRPLITHD KEQIIAKAKE IGTEDIAKSM PEFCGVISKS
360 370 380 390 400
PTVKAVKEKI EQEESYFDFS VLESAVQNAQ YLDIRQIAEQ TKKEVFEVDE
410 420 430 440 450
ITVLSANDVI LDIRSPEEVD DKPLEISGQN IILMPFYKLS SHFAELDQSK
460 470 480
NYVLYCERGV MSKLQALYLR EKGFDNVKVL NKIS
Length:484
Mass (Da):54,756
Last modified:October 3, 2006 - v1
Checksum:i8D24364396FA415B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000436 Genomic DNA. Translation: ABI25177.1.
RefSeqiYP_719112.1. NC_008309.1.

Genome annotation databases

EnsemblBacteriaiABI25177; ABI25177; HS_0902.
GeneIDi4240394.
KEGGihso:HS_0902.
PATRICi20281817. VBIHaeSom53361_0945.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000436 Genomic DNA. Translation: ABI25177.1 .
RefSeqi YP_719112.1. NC_008309.1.

3D structure databases

ProteinModelPortali Q0I3G4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 205914.HS_0902.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABI25177 ; ABI25177 ; HS_0902 .
GeneIDi 4240394.
KEGGi hso:HS_0902.
PATRICi 20281817. VBIHaeSom53361_0945.

Phylogenomic databases

eggNOGi COG0301.
HOGENOMi HOG000227469.
KOi K03151.
OrthoDBi EOG6TBHGR.

Enzyme and pathway databases

UniPathwayi UPA00060 .
BioCyci HSOM205914:GJ7V-935-MONOMER.

Family and domain databases

Gene3Di 3.40.250.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPi MF_00021. ThiI.
InterProi IPR001763. Rhodanese-like_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR026340. Thiazole_biosynth_dom.
IPR020536. ThiI_AANH.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view ]
Pfami PF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view ]
SMARTi SM00981. THUMP. 1 hit.
[Graphical view ]
SUPFAMi SSF52821. SSF52821. 1 hit.
TIGRFAMsi TIGR04271. ThiI_C_thiazole. 1 hit.
TIGR00342. TIGR00342. 1 hit.
PROSITEi PS50206. RHODANESE_3. 1 hit.
PS51165. THUMP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd."
    Challacombe J.F., Duncan A.J., Brettin T.S., Bruce D., Chertkov O., Detter J.C., Han C.S., Misra M., Richardson P., Tapia R., Thayer N., Xie G., Inzana T.J.
    J. Bacteriol. 189:1890-1898(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 129Pt.

Entry informationi

Entry nameiTHII_HISS1
AccessioniPrimary (citable) accession number: Q0I3G4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 3, 2006
Last modified: October 29, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3