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Q0I3G4

- THII_HISS1

UniProt

Q0I3G4 - THII_HISS1

Protein

tRNA sulfurtransferase

Gene

thiI

Organism
Histophilus somni (strain 129Pt) (Haemophilus somnus)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (03 Oct 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotation

    Catalytic activityi

    L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotation
    [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei265 – 2651ATPUniRule annotation
    Binding sitei287 – 2871ATP; via amide nitrogenUniRule annotation
    Binding sitei296 – 2961ATPUniRule annotation
    Active sitei456 – 4561Cysteine persulfide intermediateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi183 – 1842ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. sulfurtransferase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. thiamine biosynthetic process Source: UniProtKB-HAMAP
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
    3. thiazole biosynthetic process Source: InterPro
    4. tRNA thio-modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

    Enzyme and pathway databases

    BioCyciHSOM205914:GJ7V-935-MONOMER.
    UniPathwayiUPA00060.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
    Alternative name(s):
    Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
    Thiamine biosynthesis protein ThiIUniRule annotation
    tRNA 4-thiouridine synthaseUniRule annotation
    Gene namesi
    Name:thiIUniRule annotation
    Ordered Locus Names:HS_0902
    OrganismiHistophilus somni (strain 129Pt) (Haemophilus somnus)
    Taxonomic identifieri205914 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHistophilus
    ProteomesiUP000001970: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 484484tRNA sulfurtransferasePRO_1000074229Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi344 ↔ 456Redox-activeUniRule annotation

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi205914.HS_0902.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0I3G4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini61 – 165105THUMPUniRule annotationAdd
    BLAST
    Domaini404 – 48481RhodaneseUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ThiI family.UniRule annotation
    Contains 1 rhodanese domain.UniRule annotation
    Contains 1 THUMP domain.UniRule annotation

    Keywords - Domaini

    Redox-active center

    Phylogenomic databases

    eggNOGiCOG0301.
    HOGENOMiHOG000227469.
    KOiK03151.
    OrthoDBiEOG6TBHGR.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPiMF_00021. ThiI.
    InterProiIPR001763. Rhodanese-like_dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR026340. Thiazole_biosynth_dom.
    IPR020536. ThiI_AANH.
    IPR004114. THUMP.
    IPR003720. tRNA_STrfase.
    [Graphical view]
    PfamiPF02568. ThiI. 1 hit.
    PF02926. THUMP. 1 hit.
    [Graphical view]
    SMARTiSM00981. THUMP. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    TIGRFAMsiTIGR04271. ThiI_C_thiazole. 1 hit.
    TIGR00342. TIGR00342. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    PS51165. THUMP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q0I3G4-1 [UniParc]FASTAAdd to Basket

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    MKFIIKLFPE IMIKSESVRK RFVKILTGNI RNVLNKYDDT VAVVKHWDYI    50
    EVRSKNIENR ILLVELLGRI PGIHHFLEVE EKPFVTLHDI FEQTLSDVAT 100
    QIENKTFCVR VKRKGKHDFS SLDAERYIGG GLNQAVASAK VQLSKPDVTV 150
    RIDIENDKMM LIKARHQGIG GYPIGTQEDV LSLISGGFDS GVSSYMLIRR 200
    GSRVHYCFFN LGGATHEIGV KQMAYHIWKR FSGSHKVRFV AINFEQVVAE 250
    ILEKVDNGQM GVVLKRMMVR AASKVAQRFG IQAIVTGEAL GQVSSQTLTN 300
    LRLIDEAAES LVLRPLITHD KEQIIAKAKE IGTEDIAKSM PEFCGVISKS 350
    PTVKAVKEKI EQEESYFDFS VLESAVQNAQ YLDIRQIAEQ TKKEVFEVDE 400
    ITVLSANDVI LDIRSPEEVD DKPLEISGQN IILMPFYKLS SHFAELDQSK 450
    NYVLYCERGV MSKLQALYLR EKGFDNVKVL NKIS 484
    Length:484
    Mass (Da):54,756
    Last modified:October 3, 2006 - v1
    Checksum:i8D24364396FA415B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000436 Genomic DNA. Translation: ABI25177.1.
    RefSeqiYP_719112.1. NC_008309.1.

    Genome annotation databases

    EnsemblBacteriaiABI25177; ABI25177; HS_0902.
    GeneIDi4240394.
    KEGGihso:HS_0902.
    PATRICi20281817. VBIHaeSom53361_0945.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000436 Genomic DNA. Translation: ABI25177.1 .
    RefSeqi YP_719112.1. NC_008309.1.

    3D structure databases

    ProteinModelPortali Q0I3G4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 205914.HS_0902.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABI25177 ; ABI25177 ; HS_0902 .
    GeneIDi 4240394.
    KEGGi hso:HS_0902.
    PATRICi 20281817. VBIHaeSom53361_0945.

    Phylogenomic databases

    eggNOGi COG0301.
    HOGENOMi HOG000227469.
    KOi K03151.
    OrthoDBi EOG6TBHGR.

    Enzyme and pathway databases

    UniPathwayi UPA00060 .
    BioCyci HSOM205914:GJ7V-935-MONOMER.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPi MF_00021. ThiI.
    InterProi IPR001763. Rhodanese-like_dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR026340. Thiazole_biosynth_dom.
    IPR020536. ThiI_AANH.
    IPR004114. THUMP.
    IPR003720. tRNA_STrfase.
    [Graphical view ]
    Pfami PF02568. ThiI. 1 hit.
    PF02926. THUMP. 1 hit.
    [Graphical view ]
    SMARTi SM00981. THUMP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52821. SSF52821. 1 hit.
    TIGRFAMsi TIGR04271. ThiI_C_thiazole. 1 hit.
    TIGR00342. TIGR00342. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    PS51165. THUMP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd."
      Challacombe J.F., Duncan A.J., Brettin T.S., Bruce D., Chertkov O., Detter J.C., Han C.S., Misra M., Richardson P., Tapia R., Thayer N., Xie G., Inzana T.J.
      J. Bacteriol. 189:1890-1898(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 129Pt.

    Entry informationi

    Entry nameiTHII_HISS1
    AccessioniPrimary (citable) accession number: Q0I3G4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: October 3, 2006
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3