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Protein

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Gene

menD

Organism
Haemophilus somnus (strain 129Pt) (Histophilus somni)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC).UniRule annotation

Catalytic activityi

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotation, Mn2+UniRule annotation
  • thiamine diphosphateUniRule annotationNote: Binds 1 thiamine pyrophosphate per subunit.UniRule annotation

Pathwayi: 1,4-dihydroxy-2-naphthoate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Isochorismate synthase MenF (menF)
  2. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD)
  3. Putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH)
  4. o-succinylbenzoate synthase (menC)
  5. no protein annotated in this organism
  6. 1,4-dihydroxy-2-naphthoyl-CoA synthase (menB)
  7. no protein annotated in this organism
This subpathway is part of the pathway 1,4-dihydroxy-2-naphthoate biosynthesis, which is itself part of Quinol/quinone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate, the pathway 1,4-dihydroxy-2-naphthoate biosynthesis and in Quinol/quinone metabolism.

Pathwayi: menaquinone biosynthesis

This protein is involved in the pathway menaquinone biosynthesis, which is part of Quinol/quinone metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway menaquinone biosynthesis and in Quinol/quinone metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processMenaquinone biosynthesis
LigandMagnesium, Manganese, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

UniPathwayiUPA00079
UPA01057; UER00164

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotation (EC:2.2.1.9UniRule annotation)
Short name:
SEPHCHC synthaseUniRule annotation
Alternative name(s):
Menaquinone biosynthesis protein MenDUniRule annotation
Gene namesi
Name:menDUniRule annotation
Ordered Locus Names:HS_0617
OrganismiHaemophilus somnus (strain 129Pt) (Histophilus somni)
Taxonomic identifieri205914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHistophilus
Proteomesi
  • UP000001970 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003417561 – 5682-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseAdd BLAST568

Proteomic databases

PRIDEiQ0I316

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi205914.HS_0617

Structurei

3D structure databases

ProteinModelPortaliQ0I316
SMRiQ0I316
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TPP enzyme family. MenD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4A Bacteria
COG1165 LUCA
HOGENOMiHOG000218360
KOiK02551
OMAiIFRILPG
OrthoDBiPOG091H058U

Family and domain databases

HAMAPiMF_01659 MenD, 1 hit
InterProiView protein in InterPro
IPR004433 MenaQ_synth_MenD
IPR032264 MenD_middle
IPR029061 THDP-binding
IPR012001 Thiamin_PyroP_enz_TPP-bd_dom
IPR011766 TPP_enzyme-bd_C
PANTHERiPTHR42916 PTHR42916, 1 hit
PfamiView protein in Pfam
PF02775 TPP_enzyme_C, 1 hit
PF16582 TPP_enzyme_M_2, 1 hit
PF02776 TPP_enzyme_N, 1 hit
PIRSFiPIRSF004983 MenD, 1 hit
SUPFAMiSSF52518 SSF52518, 2 hits
TIGRFAMsiTIGR00173 menD, 1 hit

Sequencei

Sequence statusi: Complete.

Q0I316-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASVFNRCW SKVILETLSR QGVSHFCIAP GSRSTPLTLE AIRLQENGRG
60 70 80 90 100
TCHAHFDERS LGFFALGIAK ASKKPVAVIV TSGTATANLY PAIIEARQTD
110 120 130 140 150
VPLIILTADR PPELLECGAN QAILQQNMFA QYPIASINLP RPSQNYSAQW
160 170 180 190 200
LISVLDQACF RQKQGGVIHI NVPFAEPLYN ANNDEIDLHP WLSNIQSWLT
210 220 230 240 250
QNKNWIQHQE QHTEVITHKY WDQWRTKKGI IIVGRLPSEQ AMGIAEWADN
260 270 280 290 300
MGWIMITDIQ SHVKPTLPYA DIWLANQTVR KKLLQAEIVI QFGSGFIGKR
310 320 330 340 350
INQFLAEFKG EYWIIENNQK AVDPYHHAHT RFNAKPHHWL RAHPPMRKKP
360 370 380 390 400
WLLEPLALAK FCADFIKQRV GSNLNEASLA HNIELLLPKS NSVLFLGNSL
410 420 430 440 450
FVRLADALSQ PSANYPIYTN RGASGIDGLL ATAAGIAVGS EQTLVAMIGD
460 470 480 490 500
TSTLYDLNSF ALFKQLNQPA IIFVINNNGG AIFDMLPVDI AVKEKYYRMS
510 520 530 540 550
HYLEFSHIAA MFDLKYARPY TWADLATVLK QAYSRRETTV IEIKVNPNDG
560
SNIYKDLIDK IGHALIGV
Length:568
Mass (Da):63,786
Last modified:October 3, 2006 - v1
Checksum:i83A7C0CCC0908D3A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000436 Genomic DNA Translation: ABI24894.1
RefSeqiWP_011608774.1, NC_008309.1

Genome annotation databases

EnsemblBacteriaiABI24894; ABI24894; HS_0617
KEGGihso:HS_0617

Similar proteinsi

Entry informationi

Entry nameiMEND_HAES1
AccessioniPrimary (citable) accession number: Q0I316
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: October 3, 2006
Last modified: May 23, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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