Reviewed, UniProtKB/Swiss-Prot Q0I2G8 (PLSB_HAES1)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase
      Short name=GPAT
    EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	HS_0385

Organism

Haemophilus somnus (strain 129Pt) (Histophilus somni (strain 129Pt)) [Complete proteome] [HAMAP]

Taxonomic identifier

205914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Histophilus

Protein attributes

Sequence length	811 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell membrane; Peripheral membrane protein By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 811	811	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000049436
Regions
	Motif	305 – 310	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	Q0I2G8-1 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 61444F0420B80C71	FASTA	811	92,687
	10 20 30 40 50 60 MSSILNFYRN LLSVPLSFLV KNNPIPQQPI EELSLDISQP IIYLLPYTSQ TDLIIIRKNC 70 80 90 100 110 120 LSVGLPDPLE ENELGGQCLP RYVFLDEGRR FFKSKGAKSA TIQIVEKYLE LHRSLPDLNV 130 140 150 160 170 180 QLVPVSVLWG RSPGHEKQVG LPKLRLLNSI QKTAVAIWFG RDTFVRFSQA VSLRYMADEY 190 200 210 220 230 240 GTDASIALKL ARVAKIHFAK QRMSATGPRL PNRQAMFNKL LQSPAILNAI ADEAKSKRIS 250 260 270 280 290 300 KEKARKEAYK ILDEIAANVN YEGLRVADRF LGWLWNKLYQ GIDVQNAERV RKLALEGHEI 310 320 330 340 350 360 VYIPCHRSHI DYLLLSYVLY HQGLVPPHIA AGINLNFWPV GMMFRRGGAF FIRRTFKGNR 370 380 390 400 410 420 LYSTIFREYL AELFHRGYSV EFFIEGGRSR TGRLLAPKTG MMSMTVQALQ QNQIRPISVV 430 440 450 460 470 480 PVYVGYEHVL EVDTYAKELR GAAKEKENAG LVLRVIRKLR NLGQGYVNFA EPITLSNYLN 490 500 510 520 530 540 QHFPEWKDSQ LEEHSQWFNP AVNAISNQVM ININKAAAIN AMNLTGTALL SSRQRALSRE 550 560 570 580 590 600 QLLEQLKSYQ RFLQHAPYSN DIIVPTDTPE EILTHVLNLE RVGLIVEKDN FGEMLRLERS 610 620 630 640 650 660 AAVLMTYYRN NIQHVFVLPS LIASIIFHHG AIQKELVSNA ARKIYPFLKE ELFLHFSQDE 670 680 690 700 710 720 LDEYVEKIIE EFTRQKLILC AENLLSINKE RVRVLQLWMA GVREILQRYY ITVSILQDTP 730 740 750 760 770 780 NIAKATLEKE SQSIAQRLSV LHGINAPEFF DKAVFSAFIG SLRSNGYFDK NGVAITEKLN 790 800 810 DISDILDRII STEVQLTIKS AVGKHEEVQE Y

References

[1]

"Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd." Challacombe J.F., Duncan A.J., Brettin T.S., Bruce D., Chertkov O., Detter J.C., Han C.S., Misra M., Richardson P., Tapia R., Thayer N., Xie G., Inzana T.J. J. Bacteriol. 189:1890-1898(2007) [PubMed: 17172329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000436 Genomic DNA. Translation: ABI24663.1.
RefSeq	YP_718595.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0I2G8.
Genome annotation databases
GeneID	4239861.
GenomeReviews	Gene locus HS_0385 in contig CP000436_GR.
KEGG	hso:HS_0385.
NMPDR	fig|205914.1.peg.94.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2937.
HOGENOM	HBG296590.
OMA	WNKLYQG.
Enzyme and pathway databases
BioCyc	HSOM205914:HS_0385-MONOMER.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSB_HAES1

Accession

Primary (citable) accession number: Q0I2G8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 3, 2006
Last modified:	January 19, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)