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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Haemophilus somnus (strain 129Pt) (Histophilus somni)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi110ZincUniRule annotation1
Metal bindingi112ZincUniRule annotation1
Metal bindingi137ZincUniRule annotation1
Metal bindingi139ZincUniRule annotation1
Binding sitei251ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:HS_0456
OrganismiHaemophilus somnus (strain 129Pt) (Histophilus somni)
Taxonomic identifieri205914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHistophilus
Proteomesi
  • UP000001970 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000019091 – 480Glutamate--tRNA ligaseAdd BLAST480

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi205914.HS_0456

Structurei

3D structure databases

ProteinModelPortaliQ0I297
SMRiQ0I297
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi21 – 31"HIGH" regionUniRule annotationAdd BLAST11
Motifi248 – 252"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252722
KOiK01885
OMAiAYYAFDT
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.3501 hit
3.40.50.6201 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q0I297-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLEPLFNLD PNTKVRTRFA PSPTGYLHVG GARTALYSWL YAKHNQGEFV
60 70 80 90 100
LRIEDTDLER STPEATAAIL EGMEWLNLAW EHGPYFQTKR FERYNQVIDQ
110 120 130 140 150
MIEQGLAYRC YCTKERLEDL RHSQEQNKQK PRYDRYCLHQ SEYPVDAPHV
160 170 180 190 200
VRFKNPTEGT VVFDDAVRGR IEISNRELDD LIIRRTDGSP TYNFCVVVDD
210 220 230 240 250
WDMGITHVVR GEDHINNTPR QINILKALGA PIPTYAHVSM INGDDGQKLS
260 270 280 290 300
KRHGAVSVMQ YRDEGYLPEA LINYLVRLGW GHGDQEIFSR EEMIALFDIH
310 320 330 340 350
SVSKSASAFN TEKLQWLNQH YMRSLPVEQV ATHLEWHMKK HGIDYSNGPN
360 370 380 390 400
LAEIIPVLSE RCKTLKELAI SSRYFYQDVE NYDEKAVAKN FKPEAIKPLA
410 420 430 440 450
KLAEKLTALS DWTVENIHEV MSQTAQELDI GMGKVGMPFR LAVTGLGQSP
460 470 480
SMDITAKLVG KDRTISRINN AIAFIHTQNV
Length:480
Mass (Da):54,979
Last modified:October 3, 2006 - v1
Checksum:iC2915280A674E919
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000436 Genomic DNA Translation: ABI24733.1
RefSeqiWP_011608612.1, NC_008309.1

Genome annotation databases

EnsemblBacteriaiABI24733; ABI24733; HS_0456
KEGGihso:HS_0456

Similar proteinsi

Entry informationi

Entry nameiSYE_HAES1
AccessioniPrimary (citable) accession number: Q0I297
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: February 28, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome