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Q0I1V8

- SYI_HISS1

UniProt

Q0I1V8 - SYI_HISS1

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Histophilus somni (strain 129Pt) (Haemophilus somnus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (03 Oct 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei561 – 5611Aminoacyl-adenylateUniRule annotation
    Binding sitei605 – 6051ATPUniRule annotation
    Metal bindingi900 – 9001ZincUniRule annotation
    Metal bindingi903 – 9031ZincUniRule annotation
    Metal bindingi920 – 9201ZincUniRule annotation
    Metal bindingi923 – 9231ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciHSOM205914:GJ7V-192-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:HS_0186
    OrganismiHistophilus somni (strain 129Pt) (Haemophilus somnus)
    Taxonomic identifieri205914 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHistophilus
    ProteomesiUP000001970: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 937937Isoleucine--tRNA ligasePRO_1000022074Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi205914.HS_0186.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0I1V8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi602 – 6065"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q0I1V8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDYKNTLNL PETGFPMRGD LAKREPDMLK NWYDKNLYQK VREASKGKKT    50
    FILHDGPPYA NGTLHLGHAV NKILKDIIMK SKTALGFDTP YVPGWDCHGL 100
    PIELKVEGLV GKPNEKISAA EFRQACRDYA KEQVEGQKAD FIRMGVLGDW 150
    DNPYLTMNFE TEAEIIRTLG KVIQNGHLYK GSKPVHWCLD CASSLAEAEV 200
    EYEDKVSPSI YVRFAAVSAA EVEEKFNALG KGQGALSAVI WTTTPWTLPS 250
    NRAIAVNAEL EYQLVQLGNE RVILATELVQ TVQNALGIEQ IQILGSAKGG 300
    DLELIRFNHP FYDFSVPMIL GDHVTVDGGT GLVHTAPDHG QDDFVVSKKY 350
    GLEMAGLVAN DGKFISSTPF FAGKGVFEAN DLVLEKLKET GALLKLERIK 400
    HSYPHCWRHK TPIIFRATPQ WFIGMETQDL RIKALSEIKS VRWIPSWGEA 450
    RIDKMVANRP DWCISRQRTW GVPMTMFVHN ETEELHPRTL ELLEDIAKRV 500
    EKAGIQAWWD LDPAELLGEE AKTYHKVPDT LDVWFDSGST YASVVEQRPE 550
    FNGKSTDMYL EGSDQHRGWF MSSLMLSTAT NNKAPYKQVL THGFTVDEKG 600
    RKMSKSLGNV IVPSEVWNKN GADILRLWVA STDYTGEIAV SHKILNSAGD 650
    TYRRIRNTAR FLLANLNGFD PKNDLVNPEE MISLDRWAVS CALEAQNEIK 700
    EAYDNYQFHT VVQRLMRFCS IEMGSFYLDI IKDRQYTTKA DSLARRSCQT 750
    ALWHISEALV RWIAPILSFT ADEIWGYLPK LDNRAEFVFT EEFYDGLFGL 800
    DESDKLDDTY WQQLLKVRAE VNRVLEQARN DKLIGAGLEA KVTVYASEEI 850
    RPLLEQLGNE LRFVLITSQV VVKPLSKADV AESELTGLAV KVERADGEKC 900
    PRCWHFATDI GSNKEHSHIC GRCIENVEGN GEQRQFA 937
    Length:937
    Mass (Da):105,861
    Last modified:October 3, 2006 - v1
    Checksum:iC0E8BBD52F459F68
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000436 Genomic DNA. Translation: ABI24464.1.
    RefSeqiWP_011608339.1. NC_008309.1.
    YP_718393.1. NC_008309.1.

    Genome annotation databases

    EnsemblBacteriaiABI24464; ABI24464; HS_0186.
    GeneIDi4239697.
    KEGGihso:HS_0186.
    PATRICi20280280. VBIHaeSom53361_0198.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000436 Genomic DNA. Translation: ABI24464.1 .
    RefSeqi WP_011608339.1. NC_008309.1.
    YP_718393.1. NC_008309.1.

    3D structure databases

    ProteinModelPortali Q0I1V8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 205914.HS_0186.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABI24464 ; ABI24464 ; HS_0186 .
    GeneIDi 4239697.
    KEGGi hso:HS_0186.
    PATRICi 20280280. VBIHaeSom53361_0198.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci HSOM205914:GJ7V-192-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd."
      Challacombe J.F., Duncan A.J., Brettin T.S., Bruce D., Chertkov O., Detter J.C., Han C.S., Misra M., Richardson P., Tapia R., Thayer N., Xie G., Inzana T.J.
      J. Bacteriol. 189:1890-1898(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 129Pt.

    Entry informationi

    Entry nameiSYI_HISS1
    AccessioniPrimary (citable) accession number: Q0I1V8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: October 3, 2006
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3