Q0I129 (THIM_HAES1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyethylthiazole kinase
EC=2.7.1.50

Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase

Gene names

Name:	thiM
Ordered Locus Names:	HS_0092

Organism

Haemophilus somnus (strain 129Pt) (Histophilus somni (strain 129Pt)) [Complete proteome] [HAMAP]

Taxonomic identifier

205914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Histophilus

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP MF_00228
Cofactor	Magnesium By similarity. HAMAP MF_00228
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	Hydroxyethylthiazole kinase HAMAP MF_00228		PRO_0000336560
Sites
	Binding site	41	1	Substrate; via amide nitrogen By similarity
	Binding site	117	1	ATP By similarity
	Binding site	163	1	ATP By similarity
	Binding site	190	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0I129 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: CE24FC71334828B8	FASTA	266	28,152
	10 20 30 40 50 60 MNSQFLTKLR QRNPLIHNIT NIVAANFSAN GLLAIGASPI MSACIEEMEE MAKLSQSLVI 70 80 90 100 110 120 NIGTLTGKDV EAMLLAGKTA NQVGIPVVLD PVGVGATTFR QKTTALLLEQ VQFSAIRGNA 130 140 150 160 170 180 GELAYIAGVQ WSTKGVDAGK GMADIAEIAH LVARKYQCIA VISGETDYIS DGKRLAKLNN 190 200 210 220 230 240 GTPLFPKITA SGCLLSAVCS AFLAVAEQKD YFDALVEGCS AYAIAGEIAA QSLSSTQFGQ 250 260 FTLGLLDQLA SLTPEQINQY ARISYE

References

[1]

"Complete genome sequence of Haemophilus somnus (Histophilus somni) strain 129Pt and comparison to Haemophilus ducreyi 35000HP and Haemophilus influenzae Rd." Challacombe J.F., Duncan A.J., Brettin T.S., Bruce D., Chertkov O., Detter J.C., Han C.S., Misra M., Richardson P., Tapia R., Thayer N., Xie G., Inzana T.J. J. Bacteriol. 189:1890-1898(2007) [PubMed: 17172329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 129Pt.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000436 Genomic DNA. Translation: ABI24373.1.
RefSeq	YP_718299.1. NC_008309.1.
3D structure databases
ProteinModelPortal	Q0I129.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0I129.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4239601.
GenomeReviews	Gene locus HS_0092 in contig CP000436_GR.
KEGG	hso:HS_0092.
NMPDR	fig|205914.1.peg.1251.
PATRIC	20280086. VBIHaeSom53361_0101.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2145.
HOGENOM	HBG351126.
OMA	AIRGNAG.
PhylomeDB	Q0I129.
ProtClustDB	PRK09355.
Enzyme and pathway databases
BioCyc	HSOM205914:HS_0092-MONOMER.
Family and domain databases
HAMAP	MF_00228. Thz_kinase. [Tree]
InterPro	IPR000417. Hyethyz_kinase. [Graphical view]
KO	K00878.
PANTHER	PTHR20857:SF14. Hyethyz_kinase. 1 hit.
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
TIGRFAMs	TIGR00694. ThiM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THIM_HAES1

Accession

Primary (citable) accession number: Q0I129

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 3, 2006
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program