Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q0HST5 (GLGB_SHESR) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:Shewmr7_2836
OrganismShewanella sp. (strain MR-7) [Complete proteome] [HAMAP]
Taxonomic identifier60481 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length745 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7457451,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000260700

Sites

Active site4161Nucleophile By similarity
Active site4691Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0HST5 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: E9B63FDA561EC194

FASTA74585,062
        10         20         30         40         50         60 
MMTQAQTYFY DGSDVALLNG QYTDVFSLLG MHCINEGKAL VVRCFLRNAQ KVDVISLKDG 

        70         80         90        100        110        120 
RKVASLERVN EAGLFAGTLG RRVKPFLYAL RVVYPLCELD IIDPYQFGSL LDSQDLYLFG 

       130        140        150        160        170        180 
EGGSEQAYRF LGANWRQVDS VEGVHFCVWA PNAKRVSVVG DFNHWDDTRH VMRQHMANGL 

       190        200        210        220        230        240 
WEIFLPDVAE GAHYKFDLVY QNGERHAKSD PMATQMECAP NNASIVPKKH QHPWADTQWM 

       250        260        270        280        290        300 
DKRATTAWHR AAMSIYEVQL GSWRRKGEFG EQYFDYQDLI EQLIPYVKEQ GFTHIELMPV 

       310        320        330        340        350        360 
SEYPFDGSWG YQPVGLYAPT HRFGDANGLK AFIDACHQAE IGVLLDWVAA HFPKDPHGLV 

       370        380        390        400        410        420 
RFDGTCLYEH EDPRKGTHPD WDTLIYNYDR GEVRSFLLSN ACYWLREFHL DGLRLDAVSS 

       430        440        450        460        470        480 
MLYLDYSREP GQWLPNAYGG RENLEAIHFL QMLNQRLYQA FPGICMIAEE STAFAGVTKP 

       490        500        510        520        530        540 
TDCGGLGFGF KWNMGWMNDS LSYLGRDPIY RQYHHNQLTF SLMYAYSEQF MLSISHDEVV 

       550        560        570        580        590        600 
HGKGSLLHKI PGDDWQKFAT LRAYYGFMWG HPGKKLLFMG SEFAQRDEWN HNHSLDWHLL 

       610        620        630        640        650        660 
AFEPHQGVQR WLRDLNQLYR QFPALSVLDY ESQGFRWLDC DNGRDSIFSF VRYGEGSDVP 

       670        680        690        700        710        720 
LVFVVNMTPT LHQGFRIGLP QGGDFCEYLN SDSHLYGGSN QGNAGKVIAE DLPWQGMASS 

       730        740 
ALITVPPLGC LILGPATDAP RDTSL 

« Hide

References

[1]"Complete sequence of chromosome 1 of Shewanella sp. MR-7."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Nealson K., Konstantinidis K., Klappenbach J., Tiedje J., Richardson P.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MR-7.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000444 Genomic DNA. Translation: ABI43820.1.
RefSeqYP_738877.1. NC_008322.1.

3D structure databases

ProteinModelPortalQ0HST5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING60481.Shewmr7_2836.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI43820; ABI43820; Shewmr7_2836.
GeneID4255881.
KEGGshm:Shewmr7_2836.
PATRIC23590861. VBISheSp85603_2926.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAAKLLFMG.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycSSP60481:GHW6-2953-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_SHESR
AccessionPrimary (citable) accession number: Q0HST5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: October 3, 2006
Last modified: June 11, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries