Reviewed, UniProtKB/Swiss-Prot Q0HR25 (TPMT_SHESR)

Names and origin

Protein names

Recommended name:
    Thiopurine S-methyltransferase
    EC=2.1.1.67
Alternative name(s):
    Thiopurine methyltransferase

Gene names

Name:	tpm
Ordered Locus Names:	Shewmr7_3449

Organism

Shewanella sp. (strain MR-7) [Complete proteome] [HAMAP]

Taxonomic identifier

60481 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether. HAMAP MF_00812
Subcellular location	Cytoplasm By similarity HAMAP MF_00812.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Thiopurine S-methyltransferase HAMAP MF_00812		PRO_1000047225
Sites
	Binding site	10	1	S-adenosyl-L-methionine By similarity
	Binding site	45	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	66	1	S-adenosyl-L-methionine By similarity
	Binding site	123	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0HR25-1 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: A0CDA6E8A5EDE114	FASTA	218	24,864
	10 20 30 40 50 60 MEPGFWHEKW QQQLIGFHQQ DINPFLVKYW QTLALPADAK VFVPLCGKSL DMCFLAEQGH 70 80 90 100 110 120 QVIGCELNEL AVQQFFEENQ LPMKKSAEGE HQHYHTEQVS LYQGDIFTLP QSITAKVSGF 130 140 150 160 170 180 YDRAALIAWP ESMRTQYAKQ LAQLLPSGSV GLLVTLDYPQ EALSGPPFAV SPTWVETHLS 190 200 210 DDFEIQALAC QDVLADNPRF VKKEVPWLNE AVYLLRRK

References

[1]

"Complete sequence of chromosome 1 of Shewanella sp. MR-7." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Nealson K., Konstantinidis K., Klappenbach J., Tiedje J., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000444 Genomic DNA. Translation: ABI44430.1.
RefSeq	YP_739487.1.
3D structure databases
SMR	Q0HR25. Positions 1-218.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0HR25.
Genome annotation databases
GeneID	4255456.
GenomeReviews	Gene locus Shewmr7_3449 in contig CP000444_GR.
KEGG	shm:Shewmr7_3449.
NMPDR	fig|60481.10.peg.3247.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0500.
HOGENOM	HBG444929.
OMA	GKSLDMC.
Family and domain databases
HAMAP	MF_00812. Thiopur_methtran. [Tree]
InterPro	IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_SHESR

Accession

Primary (citable) accession number: Q0HR25

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 3, 2006
Last modified:	January 19, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)