Q0HPW7 (PLSB_SHESR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase
Short name=GPAT
EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	Shewmr7_3861

Organism

Shewanella sp. (strain MR-7) [Complete proteome] [HAMAP]

Taxonomic identifier

60481 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	807 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 807	807	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000049462
Regions
	Motif	308 – 313	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	Q0HPW7 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: FFC469A8ABE7F1A4	FASTA	807	91,348
	10 20 30 40 50 60 MPKQDSLWLK SLRWIQKHLV HTIVVPQDPF ADLNLDASRP LAYVMKTESL SDIAALSEIT 70 80 90 100 110 120 TKLGLPSPYE PLVVNGVVAP RVVCLEGRKP LFGERASNEP FLECFMRLLA VHKEKPELDI 130 140 150 160 170 180 QLVPVSLYWG RTPGKEDDTM KAAVLERENP TWLRKCLMIL FLGRHNFVQF SNAVSLRYMA 190 200 210 220 230 240 DEHGTDMGIA HKLARVARVH FRRQRKVMTG PVLPNRQALF HSLLKSESLR KAIQEEAASK 250 260 270 280 290 300 KISETQARET AIEYLDEIAA NYSDSLVRIA ERFLTWLWNK LYSGINIKGA EQIRQLHHDG 310 320 330 340 350 360 HEIVYVPCHR SHMDYLLLSY ILYYQGMVPP HIAAGINLNF WPAGPLFRRG GAFFIRRSFN 370 380 390 400 410 420 GNKLYTAVFR EYLDQLFAKG YSVEYFSEGG RSRTGRLLAP KTGMIAMTIN SVLRGIERPV 430 440 450 460 470 480 TLVPVYLGYD HVMEVATYHK ELSGKKKQKE SVWQVFGAIR KLGNFGQGYV NFGEPITLQN 490 500 510 520 530 540 FLNETAPNWR TEVADDPEQK PTWLTPAVNV LANRVMTRIN DAAAASSITL TSLVLLASEQ 550 560 570 580 590 600 NALERCLLER QLDLYLTLLK RVPYTSFTSV AEGDGKHLVQ QGLELNKFSI SADPLGEIVS 610 620 630 640 650 660 IDANQAITMT YYRNNIIHLF IIPSLIASCL TNNKQISRAS ILGIVNDFYP LLKAELFMGI 670 680 690 700 710 720 KDLPSYVNQV LDLFIEQGLV QESDTLSVVT ERTSQMLLLA GSVSETLQRY AIIFNLLAHR 730 740 750 760 770 780 PKMERSELES ESHLLAQRLG ALHGITAPEF YDKKLYNTLS VKLKELGYFS EKEDKSDVER 790 800 IRDQANSLLR ASVRQTIVAS VTAEHIV

References

[1]

"Complete sequence of chromosome 1 of Shewanella sp. MR-7." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Nealson K., Konstantinidis K., Klappenbach J., Tiedje J., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000444 Genomic DNA. Translation: ABI44838.1.
RefSeq	YP_739895.1. NC_008322.1.
3D structure databases
ProteinModelPortal	Q0HPW7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0HPW7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4254805.
GenomeReviews	Gene locus Shewmr7_3861 in contig CP000444_GR.
KEGG	shm:Shewmr7_3861.
NMPDR	fig|60481.10.peg.3624.
PATRIC	23592992. VBISheSp85603_3973.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2937.
HOGENOM	HBG296590.
OMA	WNKLYQG.
ProtClustDB	PRK04974.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. IPR022284. G3P_O-AcylTrfase. [Graphical view]
KO	K00631.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000437. GPAT_DHAPAT. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR03703. PlsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLSB_SHESR

Accession

Primary (citable) accession number: Q0HPW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 3, 2006
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program