Reviewed, UniProtKB/Swiss-Prot Q0HNH6 (KATG1_SHESM)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 1
      Short name=CP 1
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 1

Gene names

Name:	katG1
Ordered Locus Names:	Shewmr4_0311

Organism

Shewanella sp. (strain MR-4) [Complete proteome] [HAMAP]

Taxonomic identifier

60480 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	728 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity. HAMAP MF_01961
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 728	706	Catalase-peroxidase 1 HAMAP MF_01961		PRO_0000354925
Sites
	Active site	98	1	Proton acceptor By similarity
	Metal binding	266	1	Iron (heme axial ligand) By similarity
	Site	94	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	97 ↔ 225		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-251) By similarity
	Cross-link	225 ↔ 251		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-97) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0HNH6-1 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 6F1F22CCF3B9D472	FASTA	728	81,170
	10 20 30 40 50 60 MDKTQSSQGK CPVMHGANSA VASNNMDWWP KALNLDILHQ HDKKTDPMDP KFNYREAFNS 70 80 90 100 110 120 LDLAAVKRDL NALMTDSQDW WPADWGHYGG LMIRMAWHSA GTYRVADGRG GAGTGNQRFA 130 140 150 160 170 180 PLNSWPDNAN LDKARRLLWP IKKKYGNKLS WADLMILAGN VAYESMGLKT YGFAGGREDI 190 200 210 220 230 240 WHPEKDIYWG SEKQWLAPTE NPNSRYSGER DLENPLAAVM MGLIYVNPEG VDGKPDPLRT 250 260 270 280 290 300 AQDVRVTFAR MAMNDEETVA LTAGGHTVGK CHGNGKAQDL GPEPEGEDLE AQGLGWLNKK 310 320 330 340 350 360 GPGIGANAVT SGLEGAWTTY PTQWDNGYFH LLLNYDWELK KSPAGASQWE PINIKEEDKV 370 380 390 400 410 420 VSVGDPNRKF NPIMTDADMA MKMDPEYRKI SEKFYQDPAY FSEVFARAWF KLTHRDLGPK 430 440 450 460 470 480 SRYLGPEVPN EDLLWQDPIP SVDYRLDASE IVDLKAKLLA SGLSVSELVA TAWDSARTFR 490 500 510 520 530 540 GSDFRGGANG ARIRLAPQKD WQANEPERLQ KVLKVLTELQ ASLSKKVSIA DLIVLGGAAA 550 560 570 580 590 600 IEKAAHEAGV KVTVPFIPGR GDATQEMTDV ESFAVLEPLH DAYRNWQKKD YVVQPEEMML 610 620 630 640 650 660 DRTQLMGLTA HEMTVLVGGM RVLGTNYGGT RHGVFTDKVG VLTNDFFVNL TDMAYNWKPA 670 680 690 700 710 720 GSNLYQIVER KTGAVKWTAS RVDLVFGSNS ILRAYAEMYA QDDAKEKFVH DFVAAWTKVM NADRFDLA

References

[1]

"Complete sequence of Shewanella sp. MR-4." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Nealson K., Konstantinidis K., Klappenbach J., Tiedje J., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000446 Genomic DNA. Translation: ABI37391.1.
RefSeq	YP_732448.1.
3D structure databases
SMR	Q0HNH6. Positions 27-727.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0HNH6.
Genome annotation databases
GeneID	4251925.
GenomeReviews	Gene locus Shewmr4_0311 in contig CP000446_GR.
KEGG	she:Shewmr4_0311.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	WPNALNL.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG1_SHESM

Accession

Primary (citable) accession number: Q0HNH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	October 3, 2006
Last modified:	February 9, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)