ID   FTHS_SHESM              Reviewed;         570 AA.
AC   Q0HMS6;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 1.
DT   27-MAR-2024, entry version 87.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543};
GN   OrderedLocusNames=Shewmr4_0561;
OS   Shewanella sp. (strain MR-4).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=60480;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MR-4;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Nealson K.,
RA   Konstantinidis K., Klappenbach J., Tiedje J., Richardson P.;
RT   "Complete sequence of Shewanella sp. MR-4.";
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000446; ABI37641.1; -; Genomic_DNA.
DR   RefSeq; WP_011621363.1; NC_008321.1.
DR   AlphaFoldDB; Q0HMS6; -.
DR   SMR; Q0HMS6; -.
DR   KEGG; she:Shewmr4_0561; -.
DR   HOGENOM; CLU_003601_3_3_6; -.
DR   UniPathway; UPA00193; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT   CHAIN           1..570
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000300539"
FT   BINDING         65..72
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   570 AA;  59683 MW;  E71CD9252ABE60E6 CRC64;
     MLTDMDISRR APLKDIAVLG AEFGLLPDEM QLFGKTKAKV DLRVQQRLAE QQQGKLIIVT
     AVTPTPHGEG KTVTTIGLTQ SLKALGNKVC ACIRQPSMGP VFGVKGGAAG GGYAQVVPMQ
     ELNLHLTGDI HAVSNAHNLG AAAIASRLYH ETRLGKAEFE LQSGQNYLDI APNGIRWHRV
     VDHNDRCLRE IEVGLGENNG PAYTSGFDIT AASELMAILA LSRNLADMRA RIGKLVLAVN
     RQGAAISAED LGVAGAMTAI MADAVKPTLM QTLNGAPCLI HAGPFANIAH GNSSVIADDI
     ALKLADFVVT EGGFGSDMGF EKFCNIKARQ SGLTPSAAVL VTTLKALKAN SGLTSDADIN
     APDQARLEAG FANLNWHINN VARYGIPVVV AINRFATDTD AELNWLIEAV AGTAAFGCEL
     SEAFSQGEAG ALALAQTVMR ACEQPSEFTL LYPDDMALEA KLSTLAEVGY GAAGVSLSEA
     AKLQLQELSA LGYADLPVCM AKTPLSISHD PQLKGVPQGF IVPVRELVLN AGAGFITALV
     GNVMTMPGLG LVPGYLKIDI GADGEITGLG
//