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Q0HKU9 (PUR4_SHESM) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylformylglycinamidine synthase
Short name=FGAM synthase
Short name=FGAMS
EC=6.3.5.3
Alternative name(s):
Formylglycinamide ribotide amidotransferase
Short name=FGARAT
Formylglycinamide ribotide synthetase
Gene names
Name:purL
Ordered Locus Names:Shewmr4_1238
OrganismShewanella sp. (strain MR-4) [Complete proteome] [HAMAP]
Taxonomic identifier60480 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length1293 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419

Subunit structure

Monomer.

Subcellular location

Cytoplasm HAMAP MF_00419.

Sequence similarities

In the N-terminal section; belongs to the FGAMS family.

Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   DomainGlutamine amidotransferase
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

glutamine metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoribosylformylglycinamidine synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12931293Phosphoribosylformylglycinamidine synthase HAMAP MF_00419
PRO_0000264595

Regions

Domain1040 – 1293254Glutamine amidotransferase type-1
Nucleotide binding305 – 31612ATP Potential

Sites

Active site11331Nucleophile By similarity
Active site12581 By similarity
Active site12601 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0HKU9 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: A2B08AE29B90ECAE

FASTA1,293140,365
        10         20         30         40         50         60 
MEIIRGAPAL STFRVQKLME ACVNAALPVR QIYAEYVHLA DLSELLETNE REQLEKILTY 

        70         80         90        100        110        120 
GPAIEAHTPQ GSLLFVTPRP GTISPWSSKA TDIAHNCGLG KVKRLERGVA YYVESDTLTV 

       130        140        150        160        170        180 
EQQQTLKGLL HDRMVEVVLD DFAKADVLFK RTEPAPFKSV NVLAEGRRAL EVANVEMGLA 

       190        200        210        220        230        240 
LAEDEIDYLV ENFVRLNRNP NDIELMMFAQ ANSEHCRHKI FNADWTIDGE AQPKSLFKMI 

       250        260        270        280        290        300 
KNTFETTPDH VLSAYKDNAA VMEGSVAGRF FPDPNGVYSY HTEPMHVLMK VETHNHPTAI 

       310        320        330        340        350        360 
SPYPGAATGS GGEIRDEGAT GRGSKPKAGL TGFSVSNLKI PGFVQPWEGS YGKPDRIVSA 

       370        380        390        400        410        420 
LDIMTEGPLG GAAFNNEFGR PALLGYFRTY EQEVSSHNGV EVRGYHKPIM LAGGLGNIRE 

       430        440        450        460        470        480 
EHVQKGEITV GAKLIVLGGP AMNIGLGGGA ASSMASGQSS EDLDFASVQR ENPEMERRCQ 

       490        500        510        520        530        540 
EVIDRCWQLG DKNPIQFIHD VGAGGLSNAF PELVNDGGRG GIFNLRNVPS DEPGMSPLEI 

       550        560        570        580        590        600 
WCNESQERYV LSVAAEDLPL FTAICERERA PFAVVGEATQ EQHLTLADSH FDNNPIDLPL 

       610        620        630        640        650        660 
EVLLGKAPKM SRNVVSAKAV SPALEQSNID VKEAVKRILS LPTVADKTFL ITIGDRTVTG 

       670        680        690        700        710        720 
LVNRDQMVGP WQVPVADCAV TAASFDTYAG EAMSMGERTP LALLDFGASA RMAVAESIMN 

       730        740        750        760        770        780 
IAGADIGSFK RIKLSANWMS AAGHPGEDAG LYEAVKAVGE ELCPELSLTI PVGKDSMSMK 

       790        800        810        820        830        840 
TAWQQDGANK TVTAPMSLVI SAFGVVQDIR NTVTPELRSD KGETSLLLVD LGAGKNRLGG 

       850        860        870        880        890        900 
SCLAQVYGEL GDIAPDLDDA ALLRGFFETM QKLVAKKSVI AYHDRSDGGL FTTLVEMAFA 

       910        920        930        940        950        960 
GNTGLSIDLS ALQGTDVERL FNEELGGVLQ VSRADAELIA AQFAQAGVPC HMIGTLANDQ 

       970        980        990       1000       1010       1020 
RVTIKDGARE VFSETRVALR TLWSETTYRM QALRDNPACA LEEFKLKQDE TDLGLTVNLS 

      1030       1040       1050       1060       1070       1080 
FDPSEDVAAP YILKGAAPKM AILREQGVNS HVEMAAAFDR AGFESLDVHM SDILSGRISL 

      1090       1100       1110       1120       1130       1140 
EEFQGLVACG GFSYGDVLGA GEGWAKSILF NERARDEFSR FFERDSSFAL GVCNGCQMLS 

      1150       1160       1170       1180       1190       1200 
NLKEIIPGSE HWPRFVRNRS ERFEARFSLV EVQQSPSLFF QGMAGSRMPI AVSHGEGHAE 

      1210       1220       1230       1240       1250       1260 
FASAQALALA EASGTIALRF VNGNGEIATQ YPQNPNGSPN GLTGICTTDG RVTLMMPHPE 

      1270       1280       1290 
RVFRTVANSW HPDNWGEDSP WMRMFRNARV NLG

« Hide

References

[1]"Complete sequence of Shewanella sp. MR-4."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Nealson K., Konstantinidis K., Klappenbach J., Tiedje J., Richardson P.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MR-4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000446 Genomic DNA. Translation: ABI38318.1.
RefSeqYP_733375.1. NC_008321.1.

3D structure databases

ProteinModelPortalQ0HKU9.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ0HKU9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4251294.
GenomeReviewsGene locus Shewmr4_1238 in contig CP000446_GR.
KEGGshe:Shewmr4_1238.
PATRIC23579020. VBISheSp133532_1285.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0046.
HOGENOMHBG335309.
OMAVKAVGME.
ProtClustDBPRK05297.

Family and domain databases

HAMAPMF_00419. PurL_1.
[Tree]
InterProIPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR017926. GATASE_1.
IPR010073. PRibForGlyAmidine_synth.
IPR022940. PRibForGlyAmidine_synth_bac.
IPR016188. PurM_N-like.
[Graphical view]
KOK01952.
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMSSF56042. AIR_synth_C. 2 hits.
SSF55326. PurM_N-like. 2 hits.
TIGRFAMsTIGR01735. FGAM_synt. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR4_SHESM
AccessionPrimary (citable) accession number: Q0HKU9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: October 3, 2006
Last modified: January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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