Q0HKF7 (TGT_SHESM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Queuine tRNA-ribosyltransferase
EC=2.4.2.29

Alternative name(s):
Guanine insertion enzyme
tRNA-guanine transglycosylase

Gene names

Name:	tgt
Ordered Locus Names:	Shewmr4_1382

Organism

Shewanella sp. (strain MR-4) [Complete proteome] [HAMAP]

Taxonomic identifier

60480 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP-Rule MF_00168
Catalytic activity	Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine. HAMAP-Rule MF_00168 Guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine34 in tRNA + guanine. HAMAP-Rule MF_00168
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00168
Sequence similarities	Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
Biological process	Queuosine biosynthesis tRNA processing
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW queuine tRNA-ribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	Queuine tRNA-ribosyltransferase HAMAP-Rule MF_00168		PRO_1000016851
Sites
	Active site	89	1	Nucleophile By similarity
	Metal binding	302	1	Zinc By similarity
	Metal binding	304	1	Zinc By similarity
	Metal binding	307	1	Zinc By similarity
	Metal binding	333	1	Zinc By similarity
	Binding site	90	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0HKF7 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 16A22215264722D3	FASTA	374	42,576
	10 20 30 40 50 60 MKFELDTTDG RARRGRLIFE RGTVETPAFM PVGTYGTVKG MTPEEVRATG ADILLGNTFH 70 80 90 100 110 120 LWLRPGEEIM RKHGDLHDFM NWQRPILTDS GGFQVFSLGD IRKITEEGVH FRSPINGEKI 130 140 150 160 170 180 FLDPEKSMQI QHALGSDVVM IFDECTPYPA TEDEARKSMQ MSLRWAKRSR DEFDRLENPN 190 200 210 220 230 240 SLFGIIQGSV YEDLRDESLK GLVEIGFDGY AVGGLAVGEP KEDMHRILEH VCPQIPADKP 250 260 270 280 290 300 RYLMGVGKPE DLVEGVRRGI DMFDCVMPTR NARNGHLFTS EGVIKIRNAR HRDDTSPLDP 310 320 330 340 350 360 KCDCYTCKNY SRAYLYHLDR CNEILGARLN TIHNLRYYQM LMEGLRGAIE TGTLDAFVKD 370 FYTSQGREVP ELVD

References

[1]

"Complete sequence of Shewanella sp. MR-4." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Nealson K., Konstantinidis K., Klappenbach J., Tiedje J., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000446 Genomic DNA. Translation: ABI38460.1.
RefSeq	YP_733517.1. NC_008321.1.
3D structure databases
ProteinModelPortal	Q0HKF7.
SMR	Q0HKF7. Positions 1-363.
ModBase	Search...
Protein-protein interaction databases
STRING	60480.Shewmr4_1382.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABI38460; ABI38460; Shewmr4_1382.
GeneID	4251401.
KEGG	she:Shewmr4_1382.
PATRIC	23579354. VBISheSp133532_1435.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0343.
HOGENOM	HOG000223473.
KO	K00773.
OMA	MSPERSI.
ProtClustDB	PRK00112.
Enzyme and pathway databases
UniPathway	UPA00392.
Family and domain databases
Gene3D	3.20.20.105. 1 hit.
HAMAP	MF_00168. Q_tRNA_Tgt.
InterPro	IPR004803. Queuine_tRNA-ribosylTrfase. IPR002616. tRNA_ribo_trans. [Graphical view]
PANTHER	PTHR11962. PTHR11962. 1 hit.
Pfam	PF01702. TGT. 1 hit. [Graphical view]
SUPFAM	SSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMs	TIGR00430. Q_tRNA_tgt. 1 hit. TIGR00449. tgt_general. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TGT_SHESM

Accession

Primary (citable) accession number: Q0HKF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 3, 2006
Last modified:	May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program