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Q0HKD1

- FADJ_SHESM

UniProt

Q0HKD1 - FADJ_SHESM

Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Shewanella sp. (strain MR-4)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (03 Oct 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotation

    Catalytic activityi

    (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
    (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
    (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei116 – 1161Important for catalytic activityUniRule annotation
    Sitei138 – 1381Important for catalytic activityUniRule annotation

    GO - Molecular functioni

    1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-HAMAP
    2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-HAMAP
    3. enoyl-CoA hydratase activity Source: UniProtKB-HAMAP
    4. NAD binding Source: InterPro

    GO - Biological processi

    1. fatty acid beta-oxidation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase, Lyase, Oxidoreductase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciSSP60480:GI2N-1477-MONOMER.
    UniPathwayiUPA00659.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fatty acid oxidation complex subunit alphaUniRule annotation
    Including the following 2 domains:
    Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
    3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
    Gene namesi
    Name:fadJUniRule annotation
    Ordered Locus Names:Shewmr4_1408
    OrganismiShewanella sp. (strain MR-4)
    Taxonomic identifieri60480 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000008079: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 709709Fatty acid oxidation complex subunit alphaPRO_0000273985Add
    BLAST

    Interactioni

    Subunit structurei

    Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

    Protein-protein interaction databases

    STRINGi60480.Shewmr4_1408.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0HKD1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 188188Enoyl-CoA hydrataseUniRule annotationAdd
    BLAST
    Regioni308 – 7094023-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
    In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1250.
    HOGENOMiHOG000261346.
    KOiK01782.
    OMAiPFRYMDT.
    OrthoDBiEOG6M9F0M.

    Family and domain databases

    Gene3Di1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    HAMAPiMF_01617. FadJ.
    InterProiIPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR012802. FadJ.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00725. 3HCDH. 2 hits.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    TIGRFAMsiTIGR02440. FadJ. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q0HKD1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKTFNLTRR EDGIAILTMD VPGETMNTLK AEFGPEISEI LSEIKRDSSI    50
    RGLVLISGKK DSFVAGADIS MLDACQTAGD AKALSQQGHV VFNELEALNI 100
    PVVAAIHGAC LGGGLELALA CHQRVCSDDG KTMLGVPEVQ LGLLPGGGGT 150
    QRLPRLVGIT TALDMMLTGK QIRPKQALKM GLVNDVVPQT ILLQTAVEMA 200
    LAGKRTAKPV KKSLVNQLLE GTGFGRNIIF DQAAKQVAKK TQGNYPAPAK 250
    IIDCVRQGMA KGMQKGLEVE ASHFAELVVS KESEALRSIF FATTEMKKET 300
    GAEGATPRKV KKAVILGGGL MGGGIASVTT TKAKIPARVK DINEKGLSNA 350
    LSYAYKLLDK GVKRRHMTPA VRDNLMALMT TTTEYKGVKD ADIVVEAVFE 400
    DLALKHQMVK DIERECGEHT IFASNTSSLP IGQIAQAASR PENVIGLHYF 450
    SPVEKMPLVE VIAHAKTSPE TIATTVAFAR KQGKTPIVVQ DGAGFYVNRI 500
    LALYMNEAAQ LLLEGQSIEH LDKALVKFGF PVGPITLLDE VGIDVGAKIA 550
    PILEKELGER FKAPAAFDKL LSDDRKGRKN GKGFYQYAAG NKAPSKKKAV 600
    DESVYGVLGI KPGIDKEMSA VAERCVVQML NEAVRCLDDG IIASPRDGDI 650
    GAIFGIGFPP FLGGPFHYID TLGADNLVKI LERYQAQYGD RFEPCPRLKE 700
    MAAEKTRFF 709
    Length:709
    Mass (Da):76,316
    Last modified:October 3, 2006 - v1
    Checksum:i15B98E1FE62D375E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000446 Genomic DNA. Translation: ABI38486.1.
    RefSeqiWP_011622191.1. NC_008321.1.
    YP_733543.1. NC_008321.1.

    Genome annotation databases

    EnsemblBacteriaiABI38486; ABI38486; Shewmr4_1408.
    GeneIDi4251986.
    KEGGishe:Shewmr4_1408.
    PATRICi23579406. VBISheSp133532_1461.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000446 Genomic DNA. Translation: ABI38486.1 .
    RefSeqi WP_011622191.1. NC_008321.1.
    YP_733543.1. NC_008321.1.

    3D structure databases

    ProteinModelPortali Q0HKD1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 60480.Shewmr4_1408.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABI38486 ; ABI38486 ; Shewmr4_1408 .
    GeneIDi 4251986.
    KEGGi she:Shewmr4_1408.
    PATRICi 23579406. VBISheSp133532_1461.

    Phylogenomic databases

    eggNOGi COG1250.
    HOGENOMi HOG000261346.
    KOi K01782.
    OMAi PFRYMDT.
    OrthoDBi EOG6M9F0M.

    Enzyme and pathway databases

    UniPathwayi UPA00659 .
    BioCyci SSP60480:GI2N-1477-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    HAMAPi MF_01617. FadJ.
    InterProi IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR012802. FadJ.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00725. 3HCDH. 2 hits.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    TIGRFAMsi TIGR02440. FadJ. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MR-4.

    Entry informationi

    Entry nameiFADJ_SHESM
    AccessioniPrimary (citable) accession number: Q0HKD1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: October 3, 2006
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3