Reviewed, UniProtKB/Swiss-Prot Q0HIL5 (ASTE_SHESM)

Names and origin

Protein names

Recommended name:
    Succinylglutamate desuccinylase
    EC=3.5.1.96

Gene names

Name:	astE
Ordered Locus Names:	Shewmr4_2029

Organism

Shewanella sp. (strain MR-4) [Complete proteome] [HAMAP]

Taxonomic identifier

60480 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Transforms N(2)-succinylglutamate into succinate and glutamate. HAMAP MF_00767
Catalytic activity	N-succinyl-L-glutamate + H2O = succinate + L-glutamate. HAMAP MF_00767
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 5/5. HAMAP MF_00767
Sequence similarities	Belongs to the aspA/astE family. Succinylglutamate desuccinylase subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrolase activity, acting on ester bonds Inferred from electronic annotation. Source: HAMAP succinylglutamate desuccinylase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Succinylglutamate desuccinylase HAMAP MF_00767		PRO_0000262082
Sites
	Active site	224	1	Potential
	Metal binding	63	1	Zinc By similarity
	Metal binding	66	1	Zinc By similarity
	Metal binding	160	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0HIL5-1 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: EB013EF7E444C9B6	FASTA	344	38,716
	10 20 30 40 50 60 MLQALLDSKD FLALTLANPE TLGDEFSFTL GEHTRVEVWD TGVIVFEPVQ AQGKDVILSC 70 80 90 100 110 120 GVHGNETAPI ELCNTLIKQL LQQKIIAKQR TLFLIGNPLA INNGTRIIDE NMNRLFSGEH 130 140 150 160 170 180 SNPPGLVNPE RVRAKKLEAY VDRFFKGAAA GRQRIHYDLH TAMRASKHEK FAIYPYRPGR 190 200 210 220 230 240 AYSAEQIMFL AASGVDTVLF HHEPTTTFSY FSSEQYGADA FTIELGKVYP MGQNDMTRFI 250 260 270 280 290 300 AAQEMFMRLI TDKPLALEPF SADKVNLYQV CRVINKHFDD FEFTFATDVE NFRSFPKGFV 310 320 330 340 LAREGGQEIK VEQECESIVF PNAKVPIGNR TVICLIPSVA PDVR

References

[1]

"Complete sequence of Shewanella sp. MR-4." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Nealson K., Konstantinidis K., Klappenbach J., Tiedje J., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000446 Genomic DNA. Translation: ABI39102.1.
RefSeq	YP_734159.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4252602.
GenomeReviews	Gene locus Shewmr4_2029 in contig CP000446_GR.
KEGG	she:Shewmr4_2029.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0HIL5.
OMA	Q0HIL5. EKFAIYP.
Family and domain databases
HAMAP	MF_00767. [Tree]
InterPro	IPR007036. Aste_AspA. IPR016681. SuccinylGlu_desuccinylase. [Graphical view]
Pfam	PF04952. AstE_AspA. 1 hit. [Graphical view]
PIRSF	PIRSF017020. AstE. 1 hit.
TIGRFAMs	TIGR03242. arg_catab_astE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTE_SHESM

Accession

Primary (citable) accession number: Q0HIL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	October 3, 2006
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)