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Protein

Lipid-A-disaccharide synthase

Gene

lpxB

Organism
Shewanella sp. (strain MR-4)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciSSP60480:G1G76-2771-MONOMER
UniPathwayiUPA00359; UER00481

Protein family/group databases

CAZyiGT19 Glycosyltransferase Family 19

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid-A-disaccharide synthaseUniRule annotation (EC:2.4.1.182UniRule annotation)
Gene namesi
Name:lpxBUniRule annotation
Ordered Locus Names:Shewmr4_2628
OrganismiShewanella sp. (strain MR-4)
Taxonomic identifieri60480 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000494191 – 385Lipid-A-disaccharide synthaseAdd BLAST385

Proteomic databases

PRIDEiQ0HGW8

Structurei

3D structure databases

ProteinModelPortaliQ0HGW8
SMRiQ0HGW8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000018003
KOiK00748
OMAiPTVWAWR

Family and domain databases

HAMAPiMF_00392 LpxB, 1 hit
InterProiView protein in InterPro
IPR003835 Glyco_trans_19
PANTHERiPTHR30372 PTHR30372, 1 hit
PfamiView protein in Pfam
PF02684 LpxB, 1 hit
TIGRFAMsiTIGR00215 lpxB, 1 hit

Sequencei

Sequence statusi: Complete.

Q0HGW8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKKSQLVFA MVAGELSGDI LGAGLMAALQ KTHPNARFVG IGGPRMEALG
60 70 80 90 100
FESLFAMEEL AVMGIVEVLS RLPRLLHVRS SLIKSITELK PDCFIGIDAP
110 120 130 140 150
DFNIGLELKL KAQGIKTVHY VSPSVWAWRP KRIFKIAKAT NMVLSLLPFE
160 170 180 190 200
KAFYDKHQVP CTFVGHTLAD DIPLESDKAS ARQLLELDPD AEYLAILPGS
210 220 230 240 250
RGGELKQLAE PFVKAALLIK QQFPDIRFVT PLVNQKRREQ FEQALKAHAP
260 270 280 290 300
DLEIHMVEGK SREVMAAADG ILLASGTATL EAMLIKRPMV VAYRVSPLTY
310 320 330 340 350
QIAKTMMQVN RFSLPNLLAG RDVVPELIQH DCTPEKIAAA VGVELNRDFA
360 370 380
PIKAEFERLH QMLRCDASQK AAEAVLALVD AKDVN
Length:385
Mass (Da):42,423
Last modified:October 3, 2006 - v1
Checksum:iC508C087C9A77E78
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000446 Genomic DNA Translation: ABI39699.1
RefSeqiWP_011623380.1, NC_008321.1

Genome annotation databases

EnsemblBacteriaiABI39699; ABI39699; Shewmr4_2628
KEGGishe:Shewmr4_2628

Similar proteinsi

Entry informationi

Entry nameiLPXB_SHESM
AccessioniPrimary (citable) accession number: Q0HGW8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: May 23, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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