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Q0HFY9

- SYI_SHESM

UniProt

Q0HFY9 - SYI_SHESM

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Shewanella sp. (strain MR-4)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei564 – 5641Aminoacyl-adenylateUniRule annotation
Binding sitei608 – 6081ATPUniRule annotation
Metal bindingi903 – 9031ZincUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi923 – 9231ZincUniRule annotation
Metal bindingi926 – 9261ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSSP60480:GI2N-3075-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Shewmr4_2957
OrganismiShewanella sp. (strain MR-4)
Taxonomic identifieri60480 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000008079: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 940940Isoleucine--tRNA ligasePRO_1000022121Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi60480.Shewmr4_2957.

Structurei

3D structure databases

ProteinModelPortaliQ0HFY9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi605 – 6095"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0HFY9 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDYKFTLNL PETEFPMRGN LANREPEMLE RWTKDGLYQQ IRDSRIGRTP
60 70 80 90 100
FILHDGPPYA NGSIHIGHSV NKILKDIIVK SKTMSGFDAP YVPGWDCHGL
110 120 130 140 150
PIELKVEQKV GKPGQKISAA EFREECRKYA AEQVDGQRAD FIRLGVLGDW
160 170 180 190 200
QKPYLTMDFA TEANIVRSLS KVIENGHLHK GVKPVHWCTD CGSALAEAEV
210 220 230 240 250
EYEDKTSPAI DVAFTATDSK AVAAQFGVSD YSHPVAMVIW TTTPWTLPAN
260 270 280 290 300
RALSISPELD YSLVEFAKEG ATHAVILADV LVEACMTRYG AESHNVLGKV
310 320 330 340 350
KGAALELVRF KHPFLAFDVP AILGDHVTTD AGTGVVHTAP GHGQDDFVVG
360 370 380 390 400
QKYGLEVANP VGDNGVYKPD TEFFAGQHVF KANDNVVALL KEKGALLHHV
410 420 430 440 450
AYRHSYPHCW RHKTPIIFRA TPQWFISMDN QNLRKQALSE IEQTQWIPDW
460 470 480 490 500
GQSRIEKMVE NRPDWCISRQ RTWGVPITLF VHRETEELHP DSVSLMARVA
510 520 530 540 550
NRIEQEGIQA WWDLDAAELL GEEAEQYRKV TDTLDVWYDS GSTFASVVGA
560 570 580 590 600
RPEFHGHGVD LYLEGSDQHR GWFMSSLMIS TAMTGKAPYK QVLTHGFTVD
610 620 630 640 650
GKGRKMSKSI GNVIAPQQVT NKLGADILRL WVAATDYSGE MTVSDEILNR
660 670 680 690 700
SADAYRRIRN TARFLLANLN GFDPAKDLVA VEDMVALDRW AVRRAAALQQ
710 720 730 740 750
EIIEAYEQYN FHIVTQKLMQ FCSVELGSFY LDIIKDRQYT AKQEGHARRS
760 770 780 790 800
CQSALFHIAE AMVRWIAPIL SFTADEVWQL LPGQRNAYVF TQEWYQDLQS
810 820 830 840 850
ITLDTDLSDA YWENLLTVRN EVNKVIEQAR RDKRIGGSLE AEVTLFADAA
860 870 880 890 900
LTEQLTHIGD ELRFVLLTSE AKVLPLADAT SDAVETELAS LKLVVNATTA
910 920 930 940
EKCERCWHHR EEVGKIEAHP TLCHRCVTNI EGDGEVRLFA
Length:940
Mass (Da):105,635
Last modified:October 3, 2006 - v1
Checksum:iD17ADE09D85509CD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000446 Genomic DNA. Translation: ABI40028.1.
RefSeqiWP_011623705.1. NC_008321.1.
YP_735085.1. NC_008321.1.

Genome annotation databases

EnsemblBacteriaiABI40028; ABI40028; Shewmr4_2957.
GeneIDi4253528.
KEGGishe:Shewmr4_2957.
PATRICi23582702. VBISheSp133532_3065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000446 Genomic DNA. Translation: ABI40028.1 .
RefSeqi WP_011623705.1. NC_008321.1.
YP_735085.1. NC_008321.1.

3D structure databases

ProteinModelPortali Q0HFY9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 60480.Shewmr4_2957.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABI40028 ; ABI40028 ; Shewmr4_2957 .
GeneIDi 4253528.
KEGGi she:Shewmr4_2957.
PATRICi 23582702. VBISheSp133532_3065.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SSP60480:GI2N-3075-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MR-4.

Entry informationi

Entry nameiSYI_SHESM
AccessioniPrimary (citable) accession number: Q0HFY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2006
Last modified: October 29, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3