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Q0H8C2 - NSP2_ROTB2
Non-structural protein 2
- Names & Taxonomy
- Subcellular locationSubcell. location
- Pathology & BiotechPathol./Biotech
- PTM / Processing
- Family & Domains
- Entry information
- BLAST>sp|Q0H8C2|NSP2_ROTB2 Non-structural protein 2 OS=Rotavirus (isolate Human/Bangladesh/ADRV-N B219/2002) PE=3 SV=1 MVSIKVSLADFIVKTDEGWIPSDNCPALDRFKTKTEKELLDSIKREGADRASIRKQLFLT PISNKRLTQIGGVPVRDIRTSTTIPSSTRNLITDWLLNIFNDEESGEEVENAIASKYPDI FCSADKISRVAQRLENRRDRVHEDGFRILSATMLAIDSDIATEGKCEIVRATEDAIIAKF EPVSEHLCIGNPRGVFYKAFPIKKEQPMVYGIKALLGISNRDFIMNHGHGHLRTVPYSEI NNAIRSFAKKNEAEIKRIRSDSLSPNAGEKFINMCDMLLQKEKIETIIAKIMKNDKN
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Select a section on the left to see content.Involved in genome replication and packaging. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where replication intermediates are assembled and RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activity activities. The unwiding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. Unlike typical helicases, NSP2 requires neither a divalent cation nor a nucleotide energy source for helix destabilization. The RTPase activity may account for the absence of the 5'-terminal gamma-phosphate on the minus-strands of dsRNA genome segments By similarity.By similarity
Feature key Position(s) Length Description Graphical view Feature identifier Actions <p>Is used for enzymes and indicates the residues directly involved in catalysis.</p><p><a href='../manual/act_site' target='_top'>More...</a></p>Active sitei 231 – 231 1 For NTPase activityBy similarity
Recommended name:Non-structural protein 2 (EC:3.6.4.-)Short name:NSP2
- hydrolase activity Source: UniProtKB-KW
- nucleotide binding Source: UniProtKB-KW
- RNA binding Source: UniProtKB-KW
Rotavirus (isolate Human/Bangladesh/ADRV-N B219/2002) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219)) 348136 [NCBI] Viruses › dsRNA viruses › Reoviridae › Sedoreovirinae › Rotavirus › unclassified rotaviruses <p>Only exists in viral entries and indicates the host(s) either as a specific organism or taxonomic group of organisms that are susceptible to be infected by a virus.</p><p><a href='../manual/virus_host' target='_top'>More...</a></p>Virus hosti Homo sapiens (Human) [TaxID: 9606] UP000008656: GenomeHost cytoplasm Curated
Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.By similarity
- host cell cytoplasm Source: UniProtKB-SubCell
<p>UniProtKB Keywords constitute a <a target="_top" href="/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='../help/keywords' target='_top'>More...</a></p>Keywords - Cellular componentiHost cytoplasm
<p>Describes post-translational modifications (PTMs) and/or processing events.</p><p><a href='../manual/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi
Feature key Position(s) Length Description Graphical view Feature identifier Actions <p>Describes the extent of a polypeptide chain in the mature protein following processing.</p><p><a href='../manual/chain' target='_top'>More...</a></p>Chaini 1 – 297 297 Non-structural protein 2 PRO_0000369852 Add
<p>Provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.</p><p><a href='../manual/interaction_section' target='_top'>More...</a></p>Interactioni
<p>Provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the ‘Function’ section).</p><p><a href='../manual/subunit_structure' target='_top'>More...</a></p>Subunit structureiHomooctamer. Interacts with NSP5; this interaction leads to formation of virus factories.By similarity
Feature key Position(s) Length Description Graphical view Feature identifier Actions <p>Describes a region of interest that cannot be described in other subsections.</p><p><a href='../manual/region' target='_top'>More...</a></p>Regioni 212 – 247 36 RNA-bindingSequence Analysis Add
BLASTBelongs to the rotavirus NSP2 family.CuratedQ0H8C2-1 [UniParc]FASTAAdd to Basket
MVSIKVSLAD FIVKTDEGWI PSDNCPALDR FKTKTEKELL DSIKREGADR 50
ASIRKQLFLT PISNKRLTQI GGVPVRDIRT STTIPSSTRN LITDWLLNIF 100
NDEESGEEVE NAIASKYPDI FCSADKISRV AQRLENRRDR VHEDGFRILS 150
ATMLAIDSDI ATEGKCEIVR ATEDAIIAKF EPVSEHLCIG NPRGVFYKAF 200
PIKKEQPMVY GIKALLGISN RDFIMNHGHG HLRTVPYSEI NNAIRSFAKK 250
NEAEIKRIRS DSLSPNAGEK FINMCDMLLQ KEKIETIIAK IMKNDKN 297Length:297Mass (Da):33,554Last modified:October 3, 2006 - v1<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iC3090C54595FCC06
Sequence databasesSelect the link destinations:
DQ168035 Genomic RNA. Translation: ABA60395.1.
Sequence databasesSelect the link destinations:
DQ168035 Genomic RNA. Translation: ABA60395.1 .
3D structure databases
Database of comparative protein structure models<br/><a href='/database/63'>More..</a> ModBasei Search... Search...
Protocols and materials databases
Structural Biology Knowledgebase Search...
Family and domain databases
- "Genetic analysis of an ADRV-N-like novel rotavirus strain B219 detected in a sporadic case of adult diarrhea in Bangladesh."
Alam M.M., Kobayashi N., Ishino M., Ahmed M.S., Ahmed M.U., Paul S.K., Muzumdar B.K., Hussain Z., Wang Y.H., Naik T.N.
Arch. Virol. 152:199-208(2007) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
NSP2_ROTB2 Q0H8C2Primary (citable) accession number: Q0H8C2 Integrated into UniProtKB/Swiss-Prot: April 14, 2009 Last sequence update: October 3, 2006 Last modified: October 1, 2014 This is version 22 of the entry and version 1 of the sequence. [Complete history] Reviewed (UniProtKB/Swiss-Prot) Annotation program Viral Protein Annotation Program
- SIMILARITY commentsIndex of protein domains and families
External DataDasty 3