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Q0H8C2

- NSP2_ROTB2

UniProt

Q0H8C2 - NSP2_ROTB2

Protein

Non-structural protein 2

Gene
N/A
Organism
Rotavirus (isolate Human/Bangladesh/ADRV-N B219/2002) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219))
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 22 (01 Oct 2014)
      Sequence version 1 (03 Oct 2006)
      Previous versions | rss
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    Functioni

    Involved in genome replication and packaging. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where replication intermediates are assembled and RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activity activities. The unwiding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. Unlike typical helicases, NSP2 requires neither a divalent cation nor a nucleotide energy source for helix destabilization. The RTPase activity may account for the absence of the 5'-terminal gamma-phosphate on the minus-strands of dsRNA genome segments By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei231 – 2311For NTPase activityBy similarity

    GO - Molecular functioni

    1. hydrolase activity Source: UniProtKB-KW
    2. nucleotide binding Source: UniProtKB-KW
    3. RNA binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Non-structural protein 2 (EC:3.6.4.-)
    Short name:
    NSP2
    OrganismiRotavirus (isolate Human/Bangladesh/ADRV-N B219/2002) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219))
    Taxonomic identifieri348136 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusunclassified rotaviruses
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000008656: Genome

    Subcellular locationi

    Host cytoplasm Curated
    Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 297297Non-structural protein 2PRO_0000369852Add
    BLAST

    Interactioni

    Subunit structurei

    Homooctamer. Interacts with NSP5; this interaction leads to formation of virus factories.By similarity

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni212 – 24736RNA-bindingSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the rotavirus NSP2 family.Curated

    Sequencei

    Sequence statusi: Complete.

    Q0H8C2-1 [UniParc]FASTAAdd to Basket

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    MVSIKVSLAD FIVKTDEGWI PSDNCPALDR FKTKTEKELL DSIKREGADR    50
    ASIRKQLFLT PISNKRLTQI GGVPVRDIRT STTIPSSTRN LITDWLLNIF 100
    NDEESGEEVE NAIASKYPDI FCSADKISRV AQRLENRRDR VHEDGFRILS 150
    ATMLAIDSDI ATEGKCEIVR ATEDAIIAKF EPVSEHLCIG NPRGVFYKAF 200
    PIKKEQPMVY GIKALLGISN RDFIMNHGHG HLRTVPYSEI NNAIRSFAKK 250
    NEAEIKRIRS DSLSPNAGEK FINMCDMLLQ KEKIETIIAK IMKNDKN 297
    Length:297
    Mass (Da):33,554
    Last modified:October 3, 2006 - v1
    Checksum:iC3090C54595FCC06
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ168035 Genomic RNA. Translation: ABA60395.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ168035 Genomic RNA. Translation: ABA60395.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Genetic analysis of an ADRV-N-like novel rotavirus strain B219 detected in a sporadic case of adult diarrhea in Bangladesh."
      Alam M.M., Kobayashi N., Ishino M., Ahmed M.S., Ahmed M.U., Paul S.K., Muzumdar B.K., Hussain Z., Wang Y.H., Naik T.N.
      Arch. Virol. 152:199-208(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiNSP2_ROTB2
    AccessioniPrimary (citable) accession number: Q0H8C2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: October 3, 2006
    Last modified: October 1, 2014
    This is version 22 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3