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Q0H8C2

- NSP2_ROTB2

UniProt

Q0H8C2 - NSP2_ROTB2

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Protein

Non-structural protein 2

Gene
N/A
Organism
Rotavirus (isolate Human/Bangladesh/ADRV-N B219/2002) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219))
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Involved in genome replication and packaging. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where replication intermediates are assembled and RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activity activities. The unwiding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. Unlike typical helicases, NSP2 requires neither a divalent cation nor a nucleotide energy source for helix destabilization. The RTPase activity may account for the absence of the 5'-terminal gamma-phosphate on the minus-strands of dsRNA genome segments (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei231 – 2311For NTPase activityBy similarity

GO - Molecular functioni

  1. hydrolase activity Source: UniProtKB-KW
  2. nucleotide binding Source: UniProtKB-KW
  3. RNA binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 2 (EC:3.6.4.-)
Short name:
NSP2
OrganismiRotavirus (isolate Human/Bangladesh/ADRV-N B219/2002) (RV ADRV-N) (Rotavirus (isolate novel adult diarrhea rotavirus-B219))
Taxonomic identifieri348136 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusunclassified rotaviruses
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000008656: Genome

Subcellular locationi

Host cytoplasm Curated
Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 297297Non-structural protein 2PRO_0000369852Add
BLAST

Interactioni

Subunit structurei

Homooctamer. Interacts with NSP5; this interaction leads to formation of virus factories.By similarity

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni212 – 24736RNA-bindingSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the rotavirus NSP2 family.Curated

Sequencei

Sequence statusi: Complete.

Q0H8C2-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MVSIKVSLAD FIVKTDEGWI PSDNCPALDR FKTKTEKELL DSIKREGADR
60 70 80 90 100
ASIRKQLFLT PISNKRLTQI GGVPVRDIRT STTIPSSTRN LITDWLLNIF
110 120 130 140 150
NDEESGEEVE NAIASKYPDI FCSADKISRV AQRLENRRDR VHEDGFRILS
160 170 180 190 200
ATMLAIDSDI ATEGKCEIVR ATEDAIIAKF EPVSEHLCIG NPRGVFYKAF
210 220 230 240 250
PIKKEQPMVY GIKALLGISN RDFIMNHGHG HLRTVPYSEI NNAIRSFAKK
260 270 280 290
NEAEIKRIRS DSLSPNAGEK FINMCDMLLQ KEKIETIIAK IMKNDKN
Length:297
Mass (Da):33,554
Last modified:October 3, 2006 - v1
Checksum:iC3090C54595FCC06
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ168035 Genomic RNA. Translation: ABA60395.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ168035 Genomic RNA. Translation: ABA60395.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "Genetic analysis of an ADRV-N-like novel rotavirus strain B219 detected in a sporadic case of adult diarrhea in Bangladesh."
    Alam M.M., Kobayashi N., Ishino M., Ahmed M.S., Ahmed M.U., Paul S.K., Muzumdar B.K., Hussain Z., Wang Y.H., Naik T.N.
    Arch. Virol. 152:199-208(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNSP2_ROTB2
AccessioniPrimary (citable) accession number: Q0H8C2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: October 3, 2006
Last modified: October 29, 2014
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3