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Q0H8C2 (NSP2_ROTB2) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 21. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein attributes

Sequence length297 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in genome replication and packaging. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where replication intermediates are assembled and RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activity activities. The unwiding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. Unlike typical helicases, NSP2 requires neither a divalent cation nor a nucleotide energy source for helix destabilization. The RTPase activity may account for the absence of the 5'-terminal gamma-phosphate on the minus-strands of dsRNA genome segments By similarity.

Subunit structure

Homooctamer By similarity. Interacts with NSP5; this interaction leads to formation of virus factories By similarity.

Subcellular location

Host cytoplasm Potential. Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.

Sequence similarities

Belongs to the rotavirus NSP2 family.

Ontologies

Keywords
   Cellular componentHost cytoplasm
   LigandNucleotide-binding
RNA-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componenthost cell cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

hydrolase activity

Inferred from electronic annotation. Source: UniProtKB-KW

nucleotide binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 297297Non-structural protein 2
PRO_0000369852

Regions

Region212 – 24736RNA-binding Potential

Sites

Active site2311For NTPase activity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0H8C2 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: C3090C54595FCC06

FASTA29733,554
        10         20         30         40         50         60 
MVSIKVSLAD FIVKTDEGWI PSDNCPALDR FKTKTEKELL DSIKREGADR ASIRKQLFLT 

        70         80         90        100        110        120 
PISNKRLTQI GGVPVRDIRT STTIPSSTRN LITDWLLNIF NDEESGEEVE NAIASKYPDI 

       130        140        150        160        170        180 
FCSADKISRV AQRLENRRDR VHEDGFRILS ATMLAIDSDI ATEGKCEIVR ATEDAIIAKF 

       190        200        210        220        230        240 
EPVSEHLCIG NPRGVFYKAF PIKKEQPMVY GIKALLGISN RDFIMNHGHG HLRTVPYSEI 

       250        260        270        280        290 
NNAIRSFAKK NEAEIKRIRS DSLSPNAGEK FINMCDMLLQ KEKIETIIAK IMKNDKN 

« Hide

References

[1]"Genetic analysis of an ADRV-N-like novel rotavirus strain B219 detected in a sporadic case of adult diarrhea in Bangladesh."
Alam M.M., Kobayashi N., Ishino M., Ahmed M.S., Ahmed M.U., Paul S.K., Muzumdar B.K., Hussain Z., Wang Y.H., Naik T.N.
Arch. Virol. 152:199-208(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ168035 Genomic RNA. Translation: ABA60395.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameNSP2_ROTB2
AccessionPrimary (citable) accession number: Q0H8C2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: October 3, 2006
Last modified: April 16, 2014
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families