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Protein
Submitted name:

Thymidine kinase

Gene

tdk

Organism
Staphylococcus aureus
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891Proton acceptorUniRule annotation
Metal bindingi145 – 1451ZincUniRule annotationCombined sources
Metal bindingi148 – 1481ZincUniRule annotationCombined sources
Metal bindingi183 – 1831ZincUniRule annotationCombined sources
Metal bindingi186 – 1861ZincUniRule annotation
Metal bindingi186 – 1861Zinc; via pros nitrogenCombined sources

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 228ATPUniRule annotation
Nucleotide bindingi88 – 914ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. thymidine kinase activity Source: UniProtKB-HAMAP
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

DNA synthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, Metal-bindingUniRule annotationCombined sources, Nucleotide-binding, ZincUniRule annotationCombined sources

Enzyme and pathway databases

SABIO-RKQ0H0G9.

Names & Taxonomyi

Protein namesi
Submitted name:
Thymidine kinaseImported (EC:2.7.1.21Imported)
Gene namesi
Name:tdkUniRule annotationImported
ORF Names:CH51_11455Imported, JN533_14670Imported, QT38_11395Imported, SASCBU26_02125Imported
OrganismiStaphylococcus aureusImported
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Proteomic databases

PRIDEiQ0H0G9.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3E2IX-ray2.01A1-199[»]
ProteinModelPortaliQ0H0G9.
SMRiQ0H0G9. Positions 7-192.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ0H0G9.

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidine kinase family.UniRule annotation

Family and domain databases

HAMAPiMF_00124. Thymidine_kinase.
InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0H0G9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYETYHSGWI ECITGSMFSG KSEELIRRLR RGIYAKQKVV VFKPAIDDRY
60 70 80 90 100
HKEKVVSHNG NAIEAINISK ASEIMTHDLT NVDVIGIDEV QFFDDEIVSI
110 120 130 140 150
VEKLSADGHR VIVAGLDMDF RGEPFEPMPK LMAVSEQVTK LQAVCAVCGS
160 170 180 190
SSSRTQRLIN GKPAKIDDPI ILVGANESYE PRCRAHHIVA PSDNNKEEL
Length:199
Mass (Da):22,214
Last modified:October 3, 2006 - v1
Checksum:iC1EC76B7C5645909
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ384605 Genomic DNA. Translation: ABD37699.1.
CP007499 Genomic DNA. Translation: AIL58624.1.
CP010297 Genomic DNA. Translation: AJC35186.1.
LN626917 Genomic DNA. Translation: CEH26905.1.
CDLR01000001 Genomic DNA. Translation: CEK45138.1.
JSYA01000018 Genomic DNA. Translation: KII20577.1.
RefSeqiWP_000273356.1. NZ_KN050644.1.

Genome annotation databases

EnsemblBacteriaiAHW68308; AHW68308; X998_2098.
AIA28613; AIA28613; EX97_10880.
AID40805; AID40805; SAXN108_2364.
GeneIDi23197914.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ384605 Genomic DNA. Translation: ABD37699.1.
CP007499 Genomic DNA. Translation: AIL58624.1.
CP010297 Genomic DNA. Translation: AJC35186.1.
LN626917 Genomic DNA. Translation: CEH26905.1.
CDLR01000001 Genomic DNA. Translation: CEK45138.1.
JSYA01000018 Genomic DNA. Translation: KII20577.1.
RefSeqiWP_000273356.1. NZ_KN050644.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3E2IX-ray2.01A1-199[»]
ProteinModelPortaliQ0H0G9.
SMRiQ0H0G9. Positions 7-192.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiQ0H0G9.
ChEMBLiCHEMBL5323.

Proteomic databases

PRIDEiQ0H0G9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAHW68308; AHW68308; X998_2098.
AIA28613; AIA28613; EX97_10880.
AID40805; AID40805; SAXN108_2364.
GeneIDi23197914.

Enzyme and pathway databases

SABIO-RKQ0H0G9.

Miscellaneous databases

EvolutionaryTraceiQ0H0G9.

Family and domain databases

HAMAPiMF_00124. Thymidine_kinase.
InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Bacterial deoxyribonucleoside kinases."
    Sandrini M.P.B., Clausen A.R., Piskur J.
    Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DVI CCM 885Imported.
  2. "Deoxyribonucleoside kinases activate nucleoside antibiotics in severely pathogenic bacteria."
    Sandrini M.P., Shannon O., Clausen A.R., Bjorck L., Piskur J.
    Antimicrob. Agents Chemother. 51:2726-2732(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DVI CCM 885Imported.
  3. "Nucleoside analogues are activated by bacterial deoxyribonucleoside kinases in a species-specific manner."
    Sandrini M.P., Clausen A.R., On S.L., Aarestrup F.M., Munch-Petersen B., Piskur J.
    J. Antimicrob. Chemother. 60:510-520(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DVI CCM 885Imported.
  4. "Crystal structure of Thymidine Kinase from S. aureus."
    Lam R., Johns K., Battaile K.P., Romanov V., Lam K., Pai E.F., Chirgadze N.Y.
    Submitted (JUL-2008) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.01 ANGSTROMS) IN COMPLEX WITH ZINC.
  5. "Evolution of hypervirulence by a MRSA clone through acquisition of a transposable element."
    Benson M.A., Ohneck E.A., Ryan C., Alonzo F.III., Smith H., Narechania A., Kolokotronis S.O., Satola S.W., Uhlemann A.C., Sebra R., Deikus G., Shopsin B., Planet P.J., Torres V.J.
    Mol. Microbiol. 93:664-681(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 2395 USA500Imported.
  6. Manzoor Shahid, Zubair Saima
    Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ILRI_Eymole1/1Imported.
  7. "Genome sequencing of Staphylococcus aureus ST541."
    Moon D.C., Lim S.-K.
    Submitted (OCT-2014) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: K12PJN53Imported.
  8. "Whole Genome Sequence for Methicillin Resistant Staphylococcus aureus strain (MRSA): ATCC BAA-1680."
    Daum L.T., Bhattacharyya S., Bumah V., Meyers D.M., Enwemeka C.S.
    Submitted (OCT-2014) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 33bImported.
  9. Aslett A.Martin., De Silva Nishadi
    Submitted (OCT-2014) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: SASCBU26Imported.

Entry informationi

Entry nameiQ0H0G9_STAAU
AccessioniPrimary (citable) accession number: Q0H0G9
Entry historyi
Integrated into UniProtKB/TrEMBL: October 3, 2006
Last sequence update: October 3, 2006
Last modified: April 1, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.