ID   USP_CUCME               Reviewed;         614 AA.
AC   Q0GZS3;
DT   12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 1.
DT   03-MAR-2009, entry version 13.
DE   RecName: Full=UDP-sugar pyrophospharylase;
DE            EC=2.7.7.64;
DE   AltName: Full=UDP-galactose/glucose pyrophosphorylase;
DE            Short=UGGPase;
GN   Name=USP;
OS   Cucumis melo (Muskmelon).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Cucurbitales; Cucurbitaceae; Cucumis.
OX   NCBI_TaxID=3656;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 78-91; 120-133;
RP   288-313; 374-396; 494-502; 507-513 AND 520-528, BIOPHYSICOCHEMICAL
RP   PROPERTIES, AND DEVELOPMENTAL STAGE.
RC   STRAIN=cv. Noy Yizre'el; TISSUE=Fruit;
RX   PubMed=16829585; DOI=10.1104/pp.106.083634;
RA   Dai N., Petreikov M., Portnoy V., Katzir N., Pharr D.M.,
RA   Schaffer A.A.;
RT   "Cloning and expression analysis of a UDP-galactose/glucose
RT   pyrophosphorylase from melon fruit provides evidence for the major
RT   metabolic pathway of galactose metabolism in raffinose oligosaccharide
RT   metabolizing plants.";
RL   Plant Physiol. 142:294-304(2006).
CC   -!- FUNCTION: May function as the terminal enzyme of the myo-inositol
CC       oxidation (MIO) pathway. May also play a role in the salvage
CC       pathway for synthesis of nucleotide sugars. Can convert galactose-
CC       1-phosphate into glucose-1-phosphate by carrying out both the Gal-
CC       1-P conversion to UDP-Gal and, following an epimerase step, the
CC       subsequent reverse reaction of UDP-Glc to Glc-1-P.
CC   -!- CATALYTIC ACTIVITY: UTP + a monosaccharide 1-phosphate =
CC       diphosphate + UDP-monosaccharide.
CC   -!- COFACTOR: Magnesium or manganese (By similarity).
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.43 mM for galactose-1-phosphate;
CC         KM=0.27 mM for glucose-1-phosphate;
CC         KM=0.44 mM for UDP-galactose;
CC         KM=0.14 mM for UPD-glucose;
CC         Vmax=714 umol/min/mg enzyme for the forward reaction with
CC         galactose-1-phosphate as substrate;
CC         Vmax=222 umol/min/mg enzyme for the forward reaction with
CC         glucose-1-phosphate as substrate;
CC         Vmax=625 umol/min/mg enzyme for the reverse reaction with UDP-
CC         galactose as substrate;
CC         Vmax=238 umol/min/mg enzyme for the reverse reaction with UDP-
CC         glucose as substrate;
CC   -!- DEVELOPMENTAL STAGE: Expressed throughout fruit development.
CC   -!- SIMILARITY: Belongs to the USP family.
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DR   EMBL; DQ399739; ABD59006.1; -; mRNA.
DR   BRENDA; 2.7.7.64; 3933.
DR   GO; GO:0051748; F:UTP-monosaccharide-1-phosphate uridylyltran...; IEA:EC.
DR   GO; GO:0008152; P:metabolic process; IEA:InterPro.
DR   InterPro; IPR002618; UDPGP_trans.
DR   PANTHER; PTHR11952; UDPGP_trans; 1.
DR   Pfam; PF01704; UDPGP; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Nucleotidyltransferase; Transferase.
FT   CHAIN         1    614       UDP-sugar pyrophospharylase.
FT                                /FTId=PRO_0000289979.
SQ   SEQUENCE   614 AA;  67788 MW;  50F70438E98EE174 CRC64;
     MASSLDSAAL TLSNLSINGD FASSLPNLQK NLHLLSPQQV ELAKILLELG QSHLFEHWAE
     PGVDDNEKKA FFDQVARLNS SYPGGLASYI KTARGLLADS KEGKNPFDGF TPSVPTGEVL
     TFGDDSFVSF EDRGVREARK AAFVLVAGGL GERLGYNGIK VALPAETTTG TCFLQSYIEY
     VLALREASNR LAGESETEIP FVIMTSDDTH TRTVELLESN SYFGMKPSQV KLLKQEKVAC
     LDDNEARLAV DPHNKYRIQT KPHGHGDVHA LLYSSGLLKN WHNAGLRWVL FFQDTNGLLF
     KAIPASLGVS ATREYHVNSL AVPRKAKEAI GGITRLTHTD GRSMVINVEY NQLDPLLRAT
     GFPDGDVNNE TGYSPFPGNI NQLILELGSY IEELSKTQGA IKEFVNPKYK DATKTSFKSS
     TRLECMMQDY PKTLPPSARV GFTVMDTWVA YAPVKNNPED AAKVPKGNPY HSATSGEMAI
     YRANSLVLRK AGVKVADPVE QVFNGQEVEV WPRITWKPKW GLTFSEIKSK INGNCSISPR
     STLVIKGKNV YLKDLSLDGT LIVNADEDAE VKVEGSVHNK GWTLEPVDYK DTSVPEEIRI
     RGFRINKIEQ EERN
//