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Q0GZS3 (USP_CUCME) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-sugar pyrophosphorylase

EC=2.7.7.64
Alternative name(s):
UDP-galactose/glucose pyrophosphorylase
Short name=UGGPase
Gene names
Name:USP
OrganismCucumis melo (Muskmelon)
Taxonomic identifier3656 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeBenincaseaeCucumis

Protein attributes

Sequence length614 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May function as the terminal enzyme of the myo-inositol oxidation (MIO) pathway. May also play a role in the salvage pathway for synthesis of nucleotide sugars. Can convert galactose-1-phosphate into glucose-1-phosphate by carrying out both the Gal-1-P conversion to UDP-Gal and, following an epimerase step, the subsequent reverse reaction of UDP-Glc to Glc-1-P.

Catalytic activity

UTP + a monosaccharide 1-phosphate = diphosphate + UDP-monosaccharide.

Cofactor

Magnesium or manganese By similarity.

Developmental stage

Expressed throughout fruit development. Ref.1

Sequence similarities

Belongs to the USP family.

Biophysicochemical properties

Kinetic parameters:

KM=0.43 mM for galactose-1-phosphate Ref.1

KM=0.27 mM for glucose-1-phosphate

KM=0.44 mM for UDP-galactose

KM=0.14 mM for UPD-glucose

Vmax=714 µmol/min/mg enzyme for the forward reaction with galactose-1-phosphate as substrate

Vmax=222 µmol/min/mg enzyme for the forward reaction with glucose-1-phosphate as substrate

Vmax=625 µmol/min/mg enzyme for the reverse reaction with UDP-galactose as substrate

Vmax=238 µmol/min/mg enzyme for the reverse reaction with UDP-glucose as substrate

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 614614UDP-sugar pyrophosphorylase
PRO_0000289979

Sequences

Sequence LengthMass (Da)Tools
Q0GZS3 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: 50F70438E98EE174

FASTA61467,788
        10         20         30         40         50         60 
MASSLDSAAL TLSNLSINGD FASSLPNLQK NLHLLSPQQV ELAKILLELG QSHLFEHWAE 

        70         80         90        100        110        120 
PGVDDNEKKA FFDQVARLNS SYPGGLASYI KTARGLLADS KEGKNPFDGF TPSVPTGEVL 

       130        140        150        160        170        180 
TFGDDSFVSF EDRGVREARK AAFVLVAGGL GERLGYNGIK VALPAETTTG TCFLQSYIEY 

       190        200        210        220        230        240 
VLALREASNR LAGESETEIP FVIMTSDDTH TRTVELLESN SYFGMKPSQV KLLKQEKVAC 

       250        260        270        280        290        300 
LDDNEARLAV DPHNKYRIQT KPHGHGDVHA LLYSSGLLKN WHNAGLRWVL FFQDTNGLLF 

       310        320        330        340        350        360 
KAIPASLGVS ATREYHVNSL AVPRKAKEAI GGITRLTHTD GRSMVINVEY NQLDPLLRAT 

       370        380        390        400        410        420 
GFPDGDVNNE TGYSPFPGNI NQLILELGSY IEELSKTQGA IKEFVNPKYK DATKTSFKSS 

       430        440        450        460        470        480 
TRLECMMQDY PKTLPPSARV GFTVMDTWVA YAPVKNNPED AAKVPKGNPY HSATSGEMAI 

       490        500        510        520        530        540 
YRANSLVLRK AGVKVADPVE QVFNGQEVEV WPRITWKPKW GLTFSEIKSK INGNCSISPR 

       550        560        570        580        590        600 
STLVIKGKNV YLKDLSLDGT LIVNADEDAE VKVEGSVHNK GWTLEPVDYK DTSVPEEIRI 

       610 
RGFRINKIEQ EERN 

« Hide

References

[1]"Cloning and expression analysis of a UDP-galactose/glucose pyrophosphorylase from melon fruit provides evidence for the major metabolic pathway of galactose metabolism in raffinose oligosaccharide metabolizing plants."
Dai N., Petreikov M., Portnoy V., Katzir N., Pharr D.M., Schaffer A.A.
Plant Physiol. 142:294-304(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 78-91; 120-133; 288-313; 374-396; 494-502; 507-513 AND 520-528, BIOPHYSICOCHEMICAL PROPERTIES, DEVELOPMENTAL STAGE.
Strain: cv. Noy Yizre'el.
Tissue: Fruit.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ399739 mRNA. Translation: ABD59006.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ0GZS3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-15711.
BRENDA2.7.7.64. 1735.

Family and domain databases

Gene3D3.90.550.10. 1 hit.
InterProIPR029044. Nucleotide-diphossugar_trans.
IPR002618. UDPGP_trans.
[Graphical view]
PANTHERPTHR11952. PTHR11952. 1 hit.
PfamPF01704. UDPGP. 1 hit.
[Graphical view]
SUPFAMSSF53448. SSF53448. 1 hit.
ProtoNetSearch...

Entry information

Entry nameUSP_CUCME
AccessionPrimary (citable) accession number: Q0GZS3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 12, 2007
Last sequence update: October 3, 2006
Last modified: June 11, 2014
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families