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Q0GZS3

- USP_CUCME

UniProt

Q0GZS3 - USP_CUCME

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Protein
UDP-sugar pyrophosphorylase
Gene
USP
Organism
Cucumis melo (Muskmelon)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

May function as the terminal enzyme of the myo-inositol oxidation (MIO) pathway. May also play a role in the salvage pathway for synthesis of nucleotide sugars. Can convert galactose-1-phosphate into glucose-1-phosphate by carrying out both the Gal-1-P conversion to UDP-Gal and, following an epimerase step, the subsequent reverse reaction of UDP-Glc to Glc-1-P.

Catalytic activityi

UTP + a monosaccharide 1-phosphate = diphosphate + UDP-monosaccharide.

Cofactori

Magnesium or manganese By similarity.

Kineticsi

  1. KM=0.43 mM for galactose-1-phosphate1 Publication
  2. KM=0.27 mM for glucose-1-phosphate
  3. KM=0.44 mM for UDP-galactose
  4. KM=0.14 mM for UPD-glucose

Vmax=714 µmol/min/mg enzyme for the forward reaction with galactose-1-phosphate as substrate

Vmax=222 µmol/min/mg enzyme for the forward reaction with glucose-1-phosphate as substrate

Vmax=625 µmol/min/mg enzyme for the reverse reaction with UDP-galactose as substrate

Vmax=238 µmol/min/mg enzyme for the reverse reaction with UDP-glucose as substrate

GO - Molecular functioni

  1. UTP-monosaccharide-1-phosphate uridylyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15711.
BRENDAi2.7.7.64. 1735.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-sugar pyrophosphorylase (EC:2.7.7.64)
Alternative name(s):
UDP-galactose/glucose pyrophosphorylase
Short name:
UGGPase
Gene namesi
Name:USP
OrganismiCucumis melo (Muskmelon)
Taxonomic identifieri3656 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeBenincaseaeCucumis

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 614614UDP-sugar pyrophosphorylase
PRO_0000289979Add
BLAST

Proteomic databases

PRIDEiQ0GZS3.

Expressioni

Developmental stagei

Expressed throughout fruit development.1 Publication

Family & Domainsi

Sequence similaritiesi

Belongs to the USP family.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR002618. UDPGP_trans_fam.
[Graphical view]
PANTHERiPTHR11952. PTHR11952. 1 hit.
PfamiPF01704. UDPGP. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0GZS3-1 [UniParc]FASTAAdd to Basket

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MASSLDSAAL TLSNLSINGD FASSLPNLQK NLHLLSPQQV ELAKILLELG    50
QSHLFEHWAE PGVDDNEKKA FFDQVARLNS SYPGGLASYI KTARGLLADS 100
KEGKNPFDGF TPSVPTGEVL TFGDDSFVSF EDRGVREARK AAFVLVAGGL 150
GERLGYNGIK VALPAETTTG TCFLQSYIEY VLALREASNR LAGESETEIP 200
FVIMTSDDTH TRTVELLESN SYFGMKPSQV KLLKQEKVAC LDDNEARLAV 250
DPHNKYRIQT KPHGHGDVHA LLYSSGLLKN WHNAGLRWVL FFQDTNGLLF 300
KAIPASLGVS ATREYHVNSL AVPRKAKEAI GGITRLTHTD GRSMVINVEY 350
NQLDPLLRAT GFPDGDVNNE TGYSPFPGNI NQLILELGSY IEELSKTQGA 400
IKEFVNPKYK DATKTSFKSS TRLECMMQDY PKTLPPSARV GFTVMDTWVA 450
YAPVKNNPED AAKVPKGNPY HSATSGEMAI YRANSLVLRK AGVKVADPVE 500
QVFNGQEVEV WPRITWKPKW GLTFSEIKSK INGNCSISPR STLVIKGKNV 550
YLKDLSLDGT LIVNADEDAE VKVEGSVHNK GWTLEPVDYK DTSVPEEIRI 600
RGFRINKIEQ EERN 614
Length:614
Mass (Da):67,788
Last modified:October 3, 2006 - v1
Checksum:i50F70438E98EE174
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ399739 mRNA. Translation: ABD59006.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ399739 mRNA. Translation: ABD59006.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q0GZS3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-15711.
BRENDAi 2.7.7.64. 1735.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR029044. Nucleotide-diphossugar_trans.
IPR002618. UDPGP_trans_fam.
[Graphical view ]
PANTHERi PTHR11952. PTHR11952. 1 hit.
Pfami PF01704. UDPGP. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression analysis of a UDP-galactose/glucose pyrophosphorylase from melon fruit provides evidence for the major metabolic pathway of galactose metabolism in raffinose oligosaccharide metabolizing plants."
    Dai N., Petreikov M., Portnoy V., Katzir N., Pharr D.M., Schaffer A.A.
    Plant Physiol. 142:294-304(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 78-91; 120-133; 288-313; 374-396; 494-502; 507-513 AND 520-528, BIOPHYSICOCHEMICAL PROPERTIES, DEVELOPMENTAL STAGE.
    Strain: cv. Noy Yizre'el.
    Tissue: Fruit.

Entry informationi

Entry nameiUSP_CUCME
AccessioniPrimary (citable) accession number: Q0GZS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 12, 2007
Last sequence update: October 3, 2006
Last modified: September 3, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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