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Q0GZS3

- USP_CUCME

UniProt

Q0GZS3 - USP_CUCME

Protein

UDP-sugar pyrophosphorylase

Gene

USP

Organism
Cucumis melo (Muskmelon)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 25 (01 Oct 2014)
      Sequence version 1 (03 Oct 2006)
      Previous versions | rss
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    Functioni

    May function as the terminal enzyme of the myo-inositol oxidation (MIO) pathway. May also play a role in the salvage pathway for synthesis of nucleotide sugars. Can convert galactose-1-phosphate into glucose-1-phosphate by carrying out both the Gal-1-P conversion to UDP-Gal and, following an epimerase step, the subsequent reverse reaction of UDP-Glc to Glc-1-P.

    Catalytic activityi

    UTP + a monosaccharide 1-phosphate = diphosphate + UDP-monosaccharide.

    Cofactori

    Magnesium or manganese.By similarity

    Kineticsi

    1. KM=0.43 mM for galactose-1-phosphate1 Publication
    2. KM=0.27 mM for glucose-1-phosphate1 Publication
    3. KM=0.44 mM for UDP-galactose1 Publication
    4. KM=0.14 mM for UPD-glucose1 Publication

    Vmax=714 µmol/min/mg enzyme for the forward reaction with galactose-1-phosphate as substrate1 Publication

    Vmax=222 µmol/min/mg enzyme for the forward reaction with glucose-1-phosphate as substrate1 Publication

    Vmax=625 µmol/min/mg enzyme for the reverse reaction with UDP-galactose as substrate1 Publication

    Vmax=238 µmol/min/mg enzyme for the reverse reaction with UDP-glucose as substrate1 Publication

    GO - Molecular functioni

    1. UTP-monosaccharide-1-phosphate uridylyltransferase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-15711.
    BRENDAi2.7.7.64. 1735.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-sugar pyrophosphorylase (EC:2.7.7.64)
    Alternative name(s):
    UDP-galactose/glucose pyrophosphorylase
    Short name:
    UGGPase
    Gene namesi
    Name:USP
    OrganismiCucumis melo (Muskmelon)
    Taxonomic identifieri3656 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeBenincaseaeCucumis

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 614614UDP-sugar pyrophosphorylasePRO_0000289979Add
    BLAST

    Proteomic databases

    PRIDEiQ0GZS3.

    Expressioni

    Developmental stagei

    Expressed throughout fruit development.1 Publication

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the USP family.Curated

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR029044. Nucleotide-diphossugar_trans.
    IPR002618. UDPGP_trans_fam.
    [Graphical view]
    PANTHERiPTHR11952. PTHR11952. 1 hit.
    PfamiPF01704. UDPGP. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q0GZS3-1 [UniParc]FASTAAdd to Basket

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    MASSLDSAAL TLSNLSINGD FASSLPNLQK NLHLLSPQQV ELAKILLELG    50
    QSHLFEHWAE PGVDDNEKKA FFDQVARLNS SYPGGLASYI KTARGLLADS 100
    KEGKNPFDGF TPSVPTGEVL TFGDDSFVSF EDRGVREARK AAFVLVAGGL 150
    GERLGYNGIK VALPAETTTG TCFLQSYIEY VLALREASNR LAGESETEIP 200
    FVIMTSDDTH TRTVELLESN SYFGMKPSQV KLLKQEKVAC LDDNEARLAV 250
    DPHNKYRIQT KPHGHGDVHA LLYSSGLLKN WHNAGLRWVL FFQDTNGLLF 300
    KAIPASLGVS ATREYHVNSL AVPRKAKEAI GGITRLTHTD GRSMVINVEY 350
    NQLDPLLRAT GFPDGDVNNE TGYSPFPGNI NQLILELGSY IEELSKTQGA 400
    IKEFVNPKYK DATKTSFKSS TRLECMMQDY PKTLPPSARV GFTVMDTWVA 450
    YAPVKNNPED AAKVPKGNPY HSATSGEMAI YRANSLVLRK AGVKVADPVE 500
    QVFNGQEVEV WPRITWKPKW GLTFSEIKSK INGNCSISPR STLVIKGKNV 550
    YLKDLSLDGT LIVNADEDAE VKVEGSVHNK GWTLEPVDYK DTSVPEEIRI 600
    RGFRINKIEQ EERN 614
    Length:614
    Mass (Da):67,788
    Last modified:October 3, 2006 - v1
    Checksum:i50F70438E98EE174
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ399739 mRNA. Translation: ABD59006.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ399739 mRNA. Translation: ABD59006.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q0GZS3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-15711.
    BRENDAi 2.7.7.64. 1735.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR029044. Nucleotide-diphossugar_trans.
    IPR002618. UDPGP_trans_fam.
    [Graphical view ]
    PANTHERi PTHR11952. PTHR11952. 1 hit.
    Pfami PF01704. UDPGP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression analysis of a UDP-galactose/glucose pyrophosphorylase from melon fruit provides evidence for the major metabolic pathway of galactose metabolism in raffinose oligosaccharide metabolizing plants."
      Dai N., Petreikov M., Portnoy V., Katzir N., Pharr D.M., Schaffer A.A.
      Plant Physiol. 142:294-304(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 78-91; 120-133; 288-313; 374-396; 494-502; 507-513 AND 520-528, BIOPHYSICOCHEMICAL PROPERTIES, DEVELOPMENTAL STAGE.
      Strain: cv. Noy Yizre'el.
      Tissue: Fruit.

    Entry informationi

    Entry nameiUSP_CUCME
    AccessioniPrimary (citable) accession number: Q0GZS3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 12, 2007
    Last sequence update: October 3, 2006
    Last modified: October 1, 2014
    This is version 25 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3