Reviewed, UniProtKB/Swiss-Prot Q0GZS3 (USP_CUCME)

Names and origin

Protein names

Recommended name:
    UDP-sugar pyrophospharylase
    EC=2.7.7.64
Alternative name(s):
    UDP-galactose/glucose pyrophosphorylase
      Short name=UGGPase

Gene names

Name:

USP

Organism

Cucumis melo (Muskmelon)

Taxonomic identifier

3656 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Cucumis

Protein attributes

Sequence length	614 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May function as the terminal enzyme of the myo-inositol oxidation (MIO) pathway. May also play a role in the salvage pathway for synthesis of nucleotide sugars. Can convert galactose-1-phosphate into glucose-1-phosphate by carrying out both the Gal-1-P conversion to UDP-Gal and, following an epimerase step, the subsequent reverse reaction of UDP-Glc to Glc-1-P.
Catalytic activity	UTP + a monosaccharide 1-phosphate = diphosphate + UDP-monosaccharide.
Cofactor	Magnesium or manganese By similarity.
Developmental stage	Expressed throughout fruit development. Ref.1
Sequence similarities	Belongs to the USP family.
biophysicochemical properties	Kinetic parameters: KM=0.43 mM for galactose-1-phosphate KM=0.27 mM for glucose-1-phosphate KM=0.44 mM for UDP-galactose KM=0.14 mM for UPD-glucose Vmax=714 µmol/min/mg enzyme for the forward reaction with galactose-1-phosphate as substrate Vmax=222 µmol/min/mg enzyme for the forward reaction with glucose-1-phosphate as substrate Vmax=625 µmol/min/mg enzyme for the reverse reaction with UDP-galactose as substrate Vmax=238 µmol/min/mg enzyme for the reverse reaction with UDP-glucose as substrate

Ontologies

Keywords
Molecular function	Nucleotidyltransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	UTP-monosaccharide-1-phosphate uridylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 614	614	UDP-sugar pyrophospharylase		PRO_0000289979

Sequences

	Sequence		Length	Mass (Da)
	Q0GZS3-1 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 50F70438E98EE174	FASTA	614	67,788
	10 20 30 40 50 60 MASSLDSAAL TLSNLSINGD FASSLPNLQK NLHLLSPQQV ELAKILLELG QSHLFEHWAE 70 80 90 100 110 120 PGVDDNEKKA FFDQVARLNS SYPGGLASYI KTARGLLADS KEGKNPFDGF TPSVPTGEVL 130 140 150 160 170 180 TFGDDSFVSF EDRGVREARK AAFVLVAGGL GERLGYNGIK VALPAETTTG TCFLQSYIEY 190 200 210 220 230 240 VLALREASNR LAGESETEIP FVIMTSDDTH TRTVELLESN SYFGMKPSQV KLLKQEKVAC 250 260 270 280 290 300 LDDNEARLAV DPHNKYRIQT KPHGHGDVHA LLYSSGLLKN WHNAGLRWVL FFQDTNGLLF 310 320 330 340 350 360 KAIPASLGVS ATREYHVNSL AVPRKAKEAI GGITRLTHTD GRSMVINVEY NQLDPLLRAT 370 380 390 400 410 420 GFPDGDVNNE TGYSPFPGNI NQLILELGSY IEELSKTQGA IKEFVNPKYK DATKTSFKSS 430 440 450 460 470 480 TRLECMMQDY PKTLPPSARV GFTVMDTWVA YAPVKNNPED AAKVPKGNPY HSATSGEMAI 490 500 510 520 530 540 YRANSLVLRK AGVKVADPVE QVFNGQEVEV WPRITWKPKW GLTFSEIKSK INGNCSISPR 550 560 570 580 590 600 STLVIKGKNV YLKDLSLDGT LIVNADEDAE VKVEGSVHNK GWTLEPVDYK DTSVPEEIRI 610 RGFRINKIEQ EERN

References

[1]

"Cloning and expression analysis of a UDP-galactose/glucose pyrophosphorylase from melon fruit provides evidence for the major metabolic pathway of galactose metabolism in raffinose oligosaccharide metabolizing plants." Dai N., Petreikov M., Portnoy V., Katzir N., Pharr D.M., Schaffer A.A. Plant Physiol. 142:294-304(2006) [PubMed: 16829585] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 78-91; 120-133; 288-313; 374-396; 494-502; 507-513 AND 520-528, BIOPHYSICOCHEMICAL PROPERTIES, DEVELOPMENTAL STAGE. Strain: cv. Noy Yizre'el. Tissue: Fruit.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ399739 mRNA. Translation: ABD59006.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.7.64. 3933.
Family and domain databases
InterPro	IPR002618. UDPGP_trans. [Graphical view]
PANTHER	PTHR11952. UDPGP_trans. 1 hit.
Pfam	PF01704. UDPGP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

USP_CUCME

Accession

Primary (citable) accession number: Q0GZS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	October 3, 2006
Last modified:	March 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)