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Protein

UDP-sugar pyrophosphorylase

Gene

USP

Organism
Cucumis melo (Muskmelon)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May function as the terminal enzyme of the myo-inositol oxidation (MIO) pathway. May also play a role in the salvage pathway for synthesis of nucleotide sugars. Can convert galactose-1-phosphate into glucose-1-phosphate by carrying out both the Gal-1-P conversion to UDP-Gal and, following an epimerase step, the subsequent reverse reaction of UDP-Glc to Glc-1-P.

Catalytic activityi

UTP + a monosaccharide 1-phosphate = diphosphate + UDP-monosaccharide.

Cofactori

Mg2+By similarity, Mn2+By similarity

Kineticsi

  1. KM=0.43 mM for galactose-1-phosphate1 Publication
  2. KM=0.27 mM for glucose-1-phosphate1 Publication
  3. KM=0.44 mM for UDP-galactose1 Publication
  4. KM=0.14 mM for UPD-glucose1 Publication
  1. Vmax=714 µmol/min/mg enzyme for the forward reaction with galactose-1-phosphate as substrate1 Publication
  2. Vmax=222 µmol/min/mg enzyme for the forward reaction with glucose-1-phosphate as substrate1 Publication
  3. Vmax=625 µmol/min/mg enzyme for the reverse reaction with UDP-galactose as substrate1 Publication
  4. Vmax=238 µmol/min/mg enzyme for the reverse reaction with UDP-glucose as substrate1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15711.
BRENDAi2.7.7.64. 1735.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-sugar pyrophosphorylase (EC:2.7.7.64)
Alternative name(s):
UDP-galactose/glucose pyrophosphorylase
Short name:
UGGPase
Gene namesi
Name:USP
OrganismiCucumis melo (Muskmelon)
Taxonomic identifieri3656 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeBenincaseaeCucumis

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 614614UDP-sugar pyrophosphorylasePRO_0000289979Add
BLAST

Proteomic databases

PRIDEiQ0GZS3.

Expressioni

Developmental stagei

Expressed throughout fruit development.1 Publication

Family & Domainsi

Sequence similaritiesi

Belongs to the USP family.Curated

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR002618. UDPGP_trans_fam.
[Graphical view]
PANTHERiPTHR11952. PTHR11952. 1 hit.
PfamiPF01704. UDPGP. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0GZS3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSLDSAAL TLSNLSINGD FASSLPNLQK NLHLLSPQQV ELAKILLELG
60 70 80 90 100
QSHLFEHWAE PGVDDNEKKA FFDQVARLNS SYPGGLASYI KTARGLLADS
110 120 130 140 150
KEGKNPFDGF TPSVPTGEVL TFGDDSFVSF EDRGVREARK AAFVLVAGGL
160 170 180 190 200
GERLGYNGIK VALPAETTTG TCFLQSYIEY VLALREASNR LAGESETEIP
210 220 230 240 250
FVIMTSDDTH TRTVELLESN SYFGMKPSQV KLLKQEKVAC LDDNEARLAV
260 270 280 290 300
DPHNKYRIQT KPHGHGDVHA LLYSSGLLKN WHNAGLRWVL FFQDTNGLLF
310 320 330 340 350
KAIPASLGVS ATREYHVNSL AVPRKAKEAI GGITRLTHTD GRSMVINVEY
360 370 380 390 400
NQLDPLLRAT GFPDGDVNNE TGYSPFPGNI NQLILELGSY IEELSKTQGA
410 420 430 440 450
IKEFVNPKYK DATKTSFKSS TRLECMMQDY PKTLPPSARV GFTVMDTWVA
460 470 480 490 500
YAPVKNNPED AAKVPKGNPY HSATSGEMAI YRANSLVLRK AGVKVADPVE
510 520 530 540 550
QVFNGQEVEV WPRITWKPKW GLTFSEIKSK INGNCSISPR STLVIKGKNV
560 570 580 590 600
YLKDLSLDGT LIVNADEDAE VKVEGSVHNK GWTLEPVDYK DTSVPEEIRI
610
RGFRINKIEQ EERN
Length:614
Mass (Da):67,788
Last modified:October 3, 2006 - v1
Checksum:i50F70438E98EE174
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ399739 mRNA. Translation: ABD59006.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ399739 mRNA. Translation: ABD59006.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ0GZS3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15711.
BRENDAi2.7.7.64. 1735.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR002618. UDPGP_trans_fam.
[Graphical view]
PANTHERiPTHR11952. PTHR11952. 1 hit.
PfamiPF01704. UDPGP. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression analysis of a UDP-galactose/glucose pyrophosphorylase from melon fruit provides evidence for the major metabolic pathway of galactose metabolism in raffinose oligosaccharide metabolizing plants."
    Dai N., Petreikov M., Portnoy V., Katzir N., Pharr D.M., Schaffer A.A.
    Plant Physiol. 142:294-304(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 78-91; 120-133; 288-313; 374-396; 494-502; 507-513 AND 520-528, BIOPHYSICOCHEMICAL PROPERTIES, DEVELOPMENTAL STAGE.
    Strain: cv. Noy Yizre'el.
    Tissue: Fruit.

Entry informationi

Entry nameiUSP_CUCME
AccessioniPrimary (citable) accession number: Q0GZS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 12, 2007
Last sequence update: October 3, 2006
Last modified: November 26, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.