Q0GY44 (KBX1_TITDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Potassium channel toxin Tdi-beta-KTx Short name=TdibetaKTx
Organism	Tityus discrepans (Venezuelan scorpion)
Taxonomic identifier	57059 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Tityus

Protein attributes

Sequence length	87 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Blocks voltage-gated potassium channels By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the long chain scorpion toxin family. Class 1 subfamily.
Mass spectrometry	Molecular mass is 6822.6 Da from positions 28 - 87. Determined by MALDI. Ref.3

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Ion channel impairing toxin Neurotoxin Potassium channel inhibitor Toxin
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	potassium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 27	8			PRO_0000274676
	Chain	28 – 87	60	Potassium channel toxin Tdi-beta-KTx		PRO_0000274677
Amino acid modifications
	Disulfide bond	56 ↔ 77		By similarity
	Disulfide bond	63 ↔ 82		By similarity
	Disulfide bond	67 ↔ 84		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0GY44 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: C0A6323AF4BF0372	FASTA	87	9,833
	10 20 30 40 50 60 MERKLALLLL LGMITLASSG LREKHVQKLV TLIPNDTLRS IMKTIVHKLA KTQFGCPAYE 70 80 GYCMNHCQDI ERHDGSCHGF KCKCEKS

References

[1]	"Wide phylogenetic distribution of scorpine and long-chain beta-KTx-like peptides in scorpion venoms: identification of 'orphan' components." Diego-Garcia E., Schwartz E.F., D'Suze G., Gonzalez S.A., Batista C.V., Garcia B.I., Rodriguez de la Vega R.C., Possani L.D. Peptides 28:31-37(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 28-74. Tissue: Venom and Venom gland.
[2]	"Molecular cloning and nucleotide sequence analysis of genes from a cDNA library of the scorpion Tityus discrepans." D'Suze G., Schwartz E.F., Garcia-Gomez B.I., Sevcik C., Possani L.D. Biochimie 91:1010-1019(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Venom gland.
[3]	"Proteomic analysis of Tityus discrepans scorpion venom and amino acid sequence of novel toxins." Batista C.V.F., D'Suze G., Gomez-Lagunas F., Zamudio F.Z., Encarnacion S., Sevcik C., Possani L.D. Proteomics 6:3718-3727(2006) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY. Tissue: Venom.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ465347 mRNA. Translation: ABE98263.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

KBX1_TITDI

Accession

Primary (citable) accession number: Q0GY44

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	October 3, 2006
Last modified:	March 6, 2013
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program